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The map label for this gene is tyrS

Identifier: 121637596

GI number: 121637596

Start: 1918820

End: 1920094

Strand: Direct

Name: tyrS

Synonym: BCG_1727

Alternate gene names: 121637596

Gene position: 1918820-1920094 (Clockwise)

Preceding gene: 121637595

Following gene: 121637597

Centisome position: 43.86

GC content: 64.78

Gene sequence:

>1275_bases
ATGTCTGGCATGATCCTCGATGAGCTCAGCTGGCGCGGGTTGATCGCGCAGTCGACCGACCTCGACACGTTGGCCGCCGA
AGCACAGCGCGGGCCGATGACGGTGTACGCCGGCTTCGATCCCACCGCGCCTAGCCTGCATGCCGGACATTTGGTGCCGC
TGCTGACGTTGCGGCGCTTTCAGCGCGCCGGTCATCGCCCCATCGTGCTGGCCGGCGGGGCCACCGGCATGATCGGTGAT
CCACGTGACGTCGGCGAGCGCAGTCTCAACGAGGCCGACACCGTCGCCGAATGGACCGAACGGATCCGTGGGCAGCTGGA
GCGCTTCGTCGACTTCGACGACTCACCAATGGGCGCGATCGTCGAGAACAACCTGGAATGGACCGGCTCACTATCGGCTA
TCGAGTTTCTACGTGATATCGGCAAGCACTTCTCGGTCAACGTGATGCTGGCCCGCGACACCATCCGGCGGCGTCTGGCG
GGGGAGGGGATCTCTTACACCGAATTCAGCTACCTGTTGCTGCAGGCCAACGACTACGTCGAATTGCACCGGCGCCACGG
CTGCACGCTGCAGATCGGTGGTGCAGATCAGTGGGGCAACATCATTGCCGGCGTCCGGTTGGTGCGCCAGAAGCTCGGTG
CCACCGTGCATGCGCTTACCGTCCCCTTGGTGACCGCTGCCGACGGCACCAAGTTCGGCAAATCAACCGGCGGCGGGAGC
CTGTGGTTGGATCCCCAAATGACCAGCCCCTATGCCTGGTACCAGTACTTCGTGAACACCGCGGACGCGGATGTGATCCG
CTACCTACGGTGGTTCACCTTCTTGTCGGCCGACGAGTTGGCCGAGCTGGAACAGGCGACAGCGCAACGCCCGCAACAAC
GGGCCGCCCAGCGCCGGCTCGCCAGCGAGCTCACCGTCTTGGTGCATGGCGAGGCGGCGACCGCAGCCGTCGAGCATGCC
AGCCGGGCCCTCTTCGGTCGGGGCGAGTTGGCCCGTCTGGACGAGGCGACACTGGCTGCTGCGTTGCGGGAAACCACGGT
CGCCGAACTCAAACCGGGCAGTCCCGACGGAATCGTCGACTTATTGGTGGCCAGCGGCCTGTCGGCCAGCAAGGGCGCGG
CGCGGCGCACGATCCACGAGGGTGGGGTGTCGGTCAACAACATTCGGGTTGATAACGAGGAATGGGTGCCGCAAAGTTCG
GACTTCTTGCACGGCCGCTGGTTAGTGCTACGTCGTGGAAAGCGGAGTATCGCCGGGGTGGAACGGATTGGCTGA

Upstream 100 bases:

>100_bases
GGCCGTGGCGATTGTGGCTCACGGGTCGACCGGAGGTGTCGGCCTACCGGCGAAGCTCGCGGGCACCGGCCCGGGGAGCC
AGCGACTAGAGTCTTGCGGG

Downstream 100 bases:

>100_bases
GCCGAGCCACCACGTCCTCGACGTCCTCGGGTCCCAAGGTGATATGCGACGTGAGCGGCCCATGGAATATCGCTGGGCGG
TAGGGGAGGGCCAGCGGGGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSGMILDELSWRGLIAQSTDLDTLAAEAQRGPMTVYAGFDPTAPSLHAGHLVPLLTLRRFQRAGHRPIVLAGGATGMIGD
PRDVGERSLNEADTVAEWTERIRGQLERFVDFDDSPMGAIVENNLEWTGSLSAIEFLRDIGKHFSVNVMLARDTIRRRLA
GEGISYTEFSYLLLQANDYVELHRRHGCTLQIGGADQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGTKFGKSTGGGS
LWLDPQMTSPYAWYQYFVNTADADVIRYLRWFTFLSADELAELEQATAQRPQQRAAQRRLASELTVLVHGEAATAAVEHA
SRALFGRGELARLDEATLAAALRETTVAELKPGSPDGIVDLLVASGLSASKGAARRTIHEGGVSVNNIRVDNEEWVPQSS
DFLHGRWLVLRRGKRSIAGVERIG

Sequences:

>Translated_424_residues
MSGMILDELSWRGLIAQSTDLDTLAAEAQRGPMTVYAGFDPTAPSLHAGHLVPLLTLRRFQRAGHRPIVLAGGATGMIGD
PRDVGERSLNEADTVAEWTERIRGQLERFVDFDDSPMGAIVENNLEWTGSLSAIEFLRDIGKHFSVNVMLARDTIRRRLA
GEGISYTEFSYLLLQANDYVELHRRHGCTLQIGGADQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGTKFGKSTGGGS
LWLDPQMTSPYAWYQYFVNTADADVIRYLRWFTFLSADELAELEQATAQRPQQRAAQRRLASELTVLVHGEAATAAVEHA
SRALFGRGELARLDEATLAAALRETTVAELKPGSPDGIVDLLVASGLSASKGAARRTIHEGGVSVNNIRVDNEEWVPQSS
DFLHGRWLVLRRGKRSIAGVERIG
>Mature_423_residues
SGMILDELSWRGLIAQSTDLDTLAAEAQRGPMTVYAGFDPTAPSLHAGHLVPLLTLRRFQRAGHRPIVLAGGATGMIGDP
RDVGERSLNEADTVAEWTERIRGQLERFVDFDDSPMGAIVENNLEWTGSLSAIEFLRDIGKHFSVNVMLARDTIRRRLAG
EGISYTEFSYLLLQANDYVELHRRHGCTLQIGGADQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGTKFGKSTGGGSL
WLDPQMTSPYAWYQYFVNTADADVIRYLRWFTFLSADELAELEQATAQRPQQRAAQRRLASELTVLVHGEAATAAVEHAS
RALFGRGELARLDEATLAAALRETTVAELKPGSPDGIVDLLVASGLSASKGAARRTIHEGGVSVNNIRVDNEEWVPQSSD
FLHGRWLVLRRGKRSIAGVERIG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=378, Percent_Identity=37.8306878306878, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=47.0308788598575, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25145802, Length=376, Percent_Identity=35.6382978723404, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI25145805, Length=281, Percent_Identity=33.4519572953737, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6325160, Length=449, Percent_Identity=34.7438752783964, Blast_Score=254, Evalue=1e-68,
Organism=Drosophila melanogaster, GI20130383, Length=437, Percent_Identity=37.2997711670481, Blast_Score=261, Evalue=6e-70,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_MYCBO (P67612)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855368.1
- ProteinModelPortal:   P67612
- SMR:   P67612
- EnsemblBacteria:   EBMYCT00000015156
- GeneID:   1092664
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1715
- GeneTree:   EBGT00050000017241
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   MBOV233413:MB1715-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46331; Mature: 46200

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 36-36 BINDING 171-171 BINDING 175-175 BINDING 234-234

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGMILDELSWRGLIAQSTDLDTLAAEAQRGPMTVYAGFDPTAPSLHAGHLVPLLTLRRF
CCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
QRAGHRPIVLAGGATGMIGDPRDVGERSLNEADTVAEWTERIRGQLERFVDFDDSPMGAI
HHCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
VENNLEWTGSLSAIEFLRDIGKHFSVNVMLARDTIRRRLAGEGISYTEFSYLLLQANDYV
EECCCCCCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHCCCCCCHHEEEEEEECCCHH
ELHRRHGCTLQIGGADQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGTKFGKSTGGGS
HHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCC
LWLDPQMTSPYAWYQYFVNTADADVIRYLRWFTFLSADELAELEQATAQRPQQRAAQRRL
EEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHH
ASELTVLVHGEAATAAVEHASRALFGRGELARLDEATLAAALRETTVAELKPGSPDGIVD
HHHCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LLVASGLSASKGAARRTIHEGGVSVNNIRVDNEEWVPQSSDFLHGRWLVLRRGKRSIAGV
HHHHCCCCCCCCHHHHHHHHCCCEEEEEEECCHHCCCCCCCCCCCEEEEEECCCHHHHHH
ERIG
HHCC
>Mature Secondary Structure 
SGMILDELSWRGLIAQSTDLDTLAAEAQRGPMTVYAGFDPTAPSLHAGHLVPLLTLRRF
CCCCCCCCCCCCEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
QRAGHRPIVLAGGATGMIGDPRDVGERSLNEADTVAEWTERIRGQLERFVDFDDSPMGAI
HHCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
VENNLEWTGSLSAIEFLRDIGKHFSVNVMLARDTIRRRLAGEGISYTEFSYLLLQANDYV
EECCCCCCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHCCCCCCHHEEEEEEECCCHH
ELHRRHGCTLQIGGADQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGTKFGKSTGGGS
HHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCC
LWLDPQMTSPYAWYQYFVNTADADVIRYLRWFTFLSADELAELEQATAQRPQQRAAQRRL
EEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHH
ASELTVLVHGEAATAAVEHASRALFGRGELARLDEATLAAALRETTVAELKPGSPDGIVD
HHHCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LLVASGLSASKGAARRTIHEGGVSVNNIRVDNEEWVPQSSDFLHGRWLVLRRGKRSIAGV
HHHHCCCCCCCCHHHHHHHHCCCEEEEEEECCHHCCCCCCCCCCCEEEEEECCCHHHHHH
ERIG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972