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The map label for this gene is hisF

Identifier: 121637512

GI number: 121637512

Start: 1810100

End: 1810903

Strand: Direct

Name: hisF

Synonym: BCG_1643

Alternate gene names: 121637512

Gene position: 1810100-1810903 (Clockwise)

Preceding gene: 121637511

Following gene: 121637513

Centisome position: 41.38

GC content: 68.03

Gene sequence:

>804_bases
ATGTATGCCGACCGTGACCTTCCGGGGGCTGGGGGCCTCGCGGTACGCGTGATCCCGTGTCTGGATGTCGACGATGGGCG
GGTGGTCAAGGGAGTCAACTTCGAGAACCTCCGCGACGCCGGTGATCCCGTGGAACTCGCCGCCGTCTATGACGCGGAGG
GCGCGGACGAGTTGACCTTTCTCGACGTGACCGCGTCGTCGTCCGGAAGAGCCACCATGCTGGAGGTGGTGCGCCGCACC
GCCGAGCAGGTGTTCATCCCGCTGACGGTGGGCGGTGGGGTACGCACCGTCGCCGACGTCGATTCGCTGCTACGGGCTGG
GGCTGACAAAGTCGCCGTCAACACGGCCGCCATCGCTTGCCTGGACTTGCTGGCGGACATGGCGAGGCAGTTCGGCTCGC
AGTGCATCGTGTTGTCCGTCGACGCGCGCACAGTTCCGGTGGGATCAGCCCCGACACCGTCGGGTTGGGAGGTCACCACT
CACGGCGGTCGTCGTGGCACCGGTATGGACGCCGTGCAGTGGGCGGCCCGTGGCGCCGACCTCGGTGTGGGGGAGATCCT
GCTCAACTCGATGGACGCCGACGGCACCAAAGCCGGATTCGACCTGGCTTTGCTGCGTGCGGTCCGTGCCGCGGTCACGG
TGCCGGTAATCGCCAGCGGGGGCGCCGGTGCTGTGGAGCACTTCGCGCCAGCGGTTGCCGCGGGGGCCGATGCAGTGTTG
GCGGCCAGCGTCTTTCACTTCCGGGAGCTGACGATCGGTCAGGTGAAGGCGGCCCTGGCCGCGGAAGGAATCACCGTGCG
ATGA

Upstream 100 bases:

>100_bases
TGGACCCCTGCATCGCGTTCTGCCTTGGCCGGGCCACTGCGCGTGCATGCCCAGATCCTCGAGATTCTTGGCAGCATAGG
GGAACCAGAGGACTACTGAG

Downstream 100 bases:

>100_bases
CACTCGACCCAAAGATCGCGGCGCGGTTGAAGCGTAATGCCGACGGACTGGTTACCGCCGTCGTCCAGGAGCGGGGCAGC
GGTGACGTGCTGATGGTTGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLEVVRRT
AEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIACLDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTT
HGGRRGTGMDAVQWAARGADLGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
AASVFHFRELTIGQVKAALAAEGITVR

Sequences:

>Translated_267_residues
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLEVVRRT
AEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIACLDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTT
HGGRRGTGMDAVQWAARGADLGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
AASVFHFRELTIGQVKAALAAEGITVR
>Mature_267_residues
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLEVVRRT
AEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIACLDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTT
HGGRRGTGMDAVQWAARGADLGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
AASVFHFRELTIGQVKAALAAEGITVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=45, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=28.099173553719, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=36.3057324840764, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_MYCBO (Q7VEW8)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855284.1
- ProteinModelPortal:   Q7VEW8
- SMR:   Q7VEW8
- EnsemblBacteria:   EBMYCT00000014523
- GeneID:   1092539
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1631
- GeneTree:   EBGT00050000015857
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   MBOV233413:MB1631-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27237; Mature: 27237

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: ACT_SITE 22-22 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTF
CCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEE
LDVTASSSGRATMLEVVRRTAEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIAC
EEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHH
LDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTTHGGRRGTGMDAVQWAARGAD
HHHHHHHHHHHCCEEEEEEECCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCC
LGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCHHHH
AASVFHFRELTIGQVKAALAAEGITVR
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYADRDLPGAGGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTF
CCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEE
LDVTASSSGRATMLEVVRRTAEQVFIPLTVGGGVRTVADVDSLLRAGADKVAVNTAAIAC
EEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHH
LDLLADMARQFGSQCIVLSVDARTVPVGSAPTPSGWEVTTHGGRRGTGMDAVQWAARGAD
HHHHHHHHHHHCCEEEEEEECCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCC
LGVGEILLNSMDADGTKAGFDLALLRAVRAAVTVPVIASGGAGAVEHFAPAVAAGADAVL
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCHHHH
AASVFHFRELTIGQVKAALAAEGITVR
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972