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The map label for this gene is pyrB

Identifier: 121637310

GI number: 121637310

Start: 1578392

End: 1579351

Strand: Direct

Name: pyrB

Synonym: BCG_1441

Alternate gene names: 121637310

Gene position: 1578392-1579351 (Clockwise)

Preceding gene: 121637309

Following gene: 121637311

Centisome position: 36.08

GC content: 67.81

Gene sequence:

>960_bases
ATGACCCCAAGGCACCTGCTGACCGCCGCCGACCTCAGCCGCGACGACGCCACCGCCATCCTCGACGACGCCGACCGGTT
TGCGCAGGCGCTGGTCGGTCGCGACATCAAGAAGCTGCCGACGCTGCGGGGCCGGACCGTCGTCACGATGTTCTATGAGA
ACTCCACCCGCACCCGGGTGTCGTTCGAGGTAGCGGGTAAGTGGATGAGCGCCGACGTGATCAACGTCAGCGCTGCCGGA
TCTTCGGTAGGCAAGGGTGAGTCGCTGCGGGATACCGCGCTGACCCTGCGCGCGGCCGGGGCTGACGCGCTGATCATCCG
CCATCCCGCGTCCGGCGCCGCCCATCTGCTGGCGCAGTGGACCGGCGCCCACAACGATGGGCCGGCGGTGATCAACGCCG
GTGACGGCACTCATGAACACCCCACGCAGGCGCTGCTTGATGCGCTGACCATCCGTCAGCGCCTCGGCGGCATCGAAGGC
CGGCGCATCGTGATCGTCGGCGACATCCTGCACAGCCGGGTCGCCCGCTCCAACGTCATGCTGCTGGACACCCTGGGCGC
CGAGGTGGTGCTGGTGGCGCCACCCACATTGCTACCGGTCGGGGTGACCGGCTGGCCGGCCACCGTCTCCCACGACTTCG
ATGCCGAGCTGCCCGCCGCCGACGCGGTATTGATGCTGCGGGTACAGGCCGAGCGGATGAACGGCGGTTTTTTCCCGTCC
GTACGGGAGTACTCGGTCCGCTACGGGCTAACCGAGCGGCGCCAGGCGATGCTTCCCGGCCACGCCGTGGTGTTGCACCC
GGGACCGATGGTGCGTGGCATGGAGATCACATCTTCGGTCGCGGACTCGTCGCAATCGGCTGTGCTGCAACAGGTTTCCA
ATGGAGTCCAGGTGCGGATGGCGGTGCTGTTCCATGTGCTGGTGGGAGCGCAGGATGCCGGTAAAGAGGGTGCGGCGTGA

Upstream 100 bases:

>100_bases
GCGCCGACTATGTGGGCAAGAACGTTCCGACCTCGCGCAGCGAGAGCGTGCACGTGCGGCTGCGCGAGCACGACGGCCGT
GACGGCGTGGTGATCTCGCG

Downstream 100 bases:

>100_bases
GCGTGCTGATTCGTGGTGTGCGGCCCTACGGCGAGGGGGAGCGGGTCGACGTACTCGTCGATGACGGCCAGATCGCCCAG
ATAGGACCGGATCTGGCGAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTPRHLLTAADLSRDDATAILDDADRFAQALVGRDIKKLPTLRGRTVVTMFYENSTRTRVSFEVAGKWMSADVINVSAAG
SSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAQWTGAHNDGPAVINAGDGTHEHPTQALLDALTIRQRLGGIEG
RRIVIVGDILHSRVARSNVMLLDTLGAEVVLVAPPTLLPVGVTGWPATVSHDFDAELPAADAVLMLRVQAERMNGGFFPS
VREYSVRYGLTERRQAMLPGHAVVLHPGPMVRGMEITSSVADSSQSAVLQQVSNGVQVRMAVLFHVLVGAQDAGKEGAA

Sequences:

>Translated_319_residues
MTPRHLLTAADLSRDDATAILDDADRFAQALVGRDIKKLPTLRGRTVVTMFYENSTRTRVSFEVAGKWMSADVINVSAAG
SSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAQWTGAHNDGPAVINAGDGTHEHPTQALLDALTIRQRLGGIEG
RRIVIVGDILHSRVARSNVMLLDTLGAEVVLVAPPTLLPVGVTGWPATVSHDFDAELPAADAVLMLRVQAERMNGGFFPS
VREYSVRYGLTERRQAMLPGHAVVLHPGPMVRGMEITSSVADSSQSAVLQQVSNGVQVRMAVLFHVLVGAQDAGKEGAA
>Mature_318_residues
TPRHLLTAADLSRDDATAILDDADRFAQALVGRDIKKLPTLRGRTVVTMFYENSTRTRVSFEVAGKWMSADVINVSAAGS
SVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAQWTGAHNDGPAVINAGDGTHEHPTQALLDALTIRQRLGGIEGR
RIVIVGDILHSRVARSNVMLLDTLGAEVVLVAPPTLLPVGVTGWPATVSHDFDAELPAADAVLMLRVQAERMNGGFFPSV
REYSVRYGLTERRQAMLPGHAVVLHPGPMVRGMEITSSVADSSQSAVLQQVSNGVQVRMAVLFHVLVGAQDAGKEGAA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=37.5796178343949, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI2367364, Length=318, Percent_Identity=31.4465408805031, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786469, Length=204, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790703, Length=350, Percent_Identity=26.2857142857143, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=332, Percent_Identity=33.7349397590361, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=32.0634920634921, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24642586, Length=323, Percent_Identity=34.0557275541796, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_MYCBO (P65614)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855067.1
- ProteinModelPortal:   P65614
- SMR:   P65614
- EnsemblBacteria:   EBMYCT00000017439
- GeneID:   1090705
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1415
- GeneTree:   EBGT00050000016463
- HOGENOM:   HBG579429
- OMA:   HANIINA
- ProtClustDB:   PRK00856
- BioCyc:   MBOV233413:MB1415-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33819; Mature: 33688

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRHLLTAADLSRDDATAILDDADRFAQALVGRDIKKLPTLRGRTVVTMFYENSTRTRV
CCCHHEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCCEEE
SFEVAGKWMSADVINVSAAGSSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAQW
EEEECCCEEECEEEEEECCCCCCCCCCCCHHHEEEEEECCCCEEEEECCCCCHHHHHHHH
TGAHNDGPAVINAGDGTHEHPTQALLDALTIRQRLGGIEGRRIVIVGDILHSRVARSNVM
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCEE
LLDTLGAEVVLVAPPTLLPVGVTGWPATVSHDFDAELPAADAVLMLRVQAERMNGGFFPS
EEEECCCCEEEEECCCEEEECCCCCCCEECCCCCCCCCCCCEEEEEEEEHHHCCCCCCCC
VREYSVRYGLTERRQAMLPGHAVVLHPGPMVRGMEITSSVADSSQSAVLQQVSNGVQVRM
HHHHHHHCCCHHHHHHCCCCCEEEECCCCCEECEEHHHHHHCCHHHHHHHHHHCCHHHHH
AVLFHVLVGAQDAGKEGAA
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TPRHLLTAADLSRDDATAILDDADRFAQALVGRDIKKLPTLRGRTVVTMFYENSTRTRV
CCHHEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCCEEE
SFEVAGKWMSADVINVSAAGSSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLAQW
EEEECCCEEECEEEEEECCCCCCCCCCCCHHHEEEEEECCCCEEEEECCCCCHHHHHHHH
TGAHNDGPAVINAGDGTHEHPTQALLDALTIRQRLGGIEGRRIVIVGDILHSRVARSNVM
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCEE
LLDTLGAEVVLVAPPTLLPVGVTGWPATVSHDFDAELPAADAVLMLRVQAERMNGGFFPS
EEEECCCCEEEEECCCEEEECCCCCCCEECCCCCCCCCCCCEEEEEEEEHHHCCCCCCCC
VREYSVRYGLTERRQAMLPGHAVVLHPGPMVRGMEITSSVADSSQSAVLQQVSNGVQVRM
HHHHHHHCCCHHHHHHCCCCCEEEECCCCCEECEEHHHHHHCCHHHHHHHHHHCCHHHHH
AVLFHVLVGAQDAGKEGAA
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972