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The map label for this gene is fadE14

Identifier: 121637277

GI number: 121637277

Start: 1537360

End: 1538520

Strand: Direct

Name: fadE14

Synonym: BCG_1408

Alternate gene names: 121637277

Gene position: 1537360-1538520 (Clockwise)

Preceding gene: 121637276

Following gene: 121637279

Centisome position: 35.14

GC content: 64.94

Gene sequence:

>1161_bases
ATGACGGCCGGCTCCGACCTCGACGACTTCCGCGGTTTGCTCGCCAAAGCGTTCGACGAGCGGGTGGTGGCATGGACCGC
AGAAGCGGAAGCGCAGGAACGTTTTCCGCGCCAGTTGATCGAACACCTGGGTGTCTGCGGCGTATTCGATGCGAAGTGGG
CGACCGACGCCCGTCCCGACGTCGGTAAACTCGTCGAACTCGCTTTCGCGTTGGGCCAGCTGGCCTCTGCCGGCATCGGT
GTGGGTGTCAGCTTGCATGACTCGGCGATCGCGATTTTGCGCCGGTTTGGTAAGTCGGACTACTTGCGGGATATCTGCGA
TCAGGCGATCCGTGGCGCCGCGGTGCTGTGCATCGGAGCCTCGGAGGAGTCCGGCGGATCCGACCTGCAGATCGTCGAAA
CCGAGATACGGTCCCGTGACGGTGGTTTCGAGGTCCGCGGCGTCAAGAAATTCGTGTCGCTGTCTCCGATCGCCGACCAC
ATCATGGTGGTGGCCCGCAGCGTCGACCACGATCCGACCAGTAGGCACGGCAATGTCGCGGTCGTGGCCGTGCCGGCCGC
ACAAGTCAGCGTGCAGACCCCCTACCGCAAGGTCGGTGCGGGACCGCTGGATACCGCCGCGGTCTGCATCGACACCTGGG
TACCGGCCGATGCACTGGTTGCGCGGGCCGGCACGGGGCTGGCAGCCATCAGTTGGGGACTGGCTCATGAGCGGATGTCG
ATCGCCGGGCAGATCGCAGCGTCGTGTCAACGGGCGATCGGAATCACCCTGGCCCGCATGATGAGTCGACGTCAGTTCGG
TCAGACGCTGTTCGAACACCAGGCGCTGCGGCTGCGTATGGCGGACCTGCAGGCGCGTGTCGATCTGCTGCGGTACGCGC
TGCACGGCATCGCTGAACAGGGGAGACTGGAACTGCGCACGGCGGCAGCGGTCAAAGTCACCGCCGCCCGGCTCGGTGAG
GAAGTCATCTCCGAATGCATGCACATCTTCGGTGGGGCGGGTTATCTTGTCGACGAAACGACGCTTGGCAAATGGTGGCG
GGACATGAAGCTCGCCCGGGTCGGCGGCGGCACCGACGAGGTGCTGTGGGAATTGGTGGCTGCCGGCATGACGCCCGATC
ACGACGGTTACGCAGCCGTGGTCGGAGCTTCCAAAGCGTAG

Upstream 100 bases:

>100_bases
CGTCCGACGTCGTCTTCGTGTCGCCTGGATCACTGCCCCGGACGTCGTCTGGAAAACTGCGCCGCTTGGCAGTCCGGCGC
TCCCTGGAGATGGCGGACTG

Downstream 100 bases:

>100_bases
AGCGCCATGCGCCGGTTTGTCGTGTCATGCTCACCGAGGAACTTGCATCCGGCCCACTCACACAACCGACGGGTCGCGGT
GTTGCGGTGATCGGGGTCGA

Product: putative acyl-CoA dehydrogenase fadE14

Products: NA

Alternate protein names: Acyl-ACP dehydrogenase MbtN; Mycobactin synthase protein N

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTAGSDLDDFRGLLAKAFDERVVAWTAEAEAQERFPRQLIEHLGVCGVFDAKWATDARPDVGKLVELAFALGQLASAGIG
VGVSLHDSAIAILRRFGKSDYLRDICDQAIRGAAVLCIGASEESGGSDLQIVETEIRSRDGGFEVRGVKKFVSLSPIADH
IMVVARSVDHDPTSRHGNVAVVAVPAAQVSVQTPYRKVGAGPLDTAAVCIDTWVPADALVARAGTGLAAISWGLAHERMS
IAGQIAASCQRAIGITLARMMSRRQFGQTLFEHQALRLRMADLQARVDLLRYALHGIAEQGRLELRTAAAVKVTAARLGE
EVISECMHIFGGAGYLVDETTLGKWWRDMKLARVGGGTDEVLWELVAAGMTPDHDGYAAVVGASKA

Sequences:

>Translated_386_residues
MTAGSDLDDFRGLLAKAFDERVVAWTAEAEAQERFPRQLIEHLGVCGVFDAKWATDARPDVGKLVELAFALGQLASAGIG
VGVSLHDSAIAILRRFGKSDYLRDICDQAIRGAAVLCIGASEESGGSDLQIVETEIRSRDGGFEVRGVKKFVSLSPIADH
IMVVARSVDHDPTSRHGNVAVVAVPAAQVSVQTPYRKVGAGPLDTAAVCIDTWVPADALVARAGTGLAAISWGLAHERMS
IAGQIAASCQRAIGITLARMMSRRQFGQTLFEHQALRLRMADLQARVDLLRYALHGIAEQGRLELRTAAAVKVTAARLGE
EVISECMHIFGGAGYLVDETTLGKWWRDMKLARVGGGTDEVLWELVAAGMTPDHDGYAAVVGASKA
>Mature_385_residues
TAGSDLDDFRGLLAKAFDERVVAWTAEAEAQERFPRQLIEHLGVCGVFDAKWATDARPDVGKLVELAFALGQLASAGIGV
GVSLHDSAIAILRRFGKSDYLRDICDQAIRGAAVLCIGASEESGGSDLQIVETEIRSRDGGFEVRGVKKFVSLSPIADHI
MVVARSVDHDPTSRHGNVAVVAVPAAQVSVQTPYRKVGAGPLDTAAVCIDTWVPADALVARAGTGLAAISWGLAHERMSI
AGQIAASCQRAIGITLARMMSRRQFGQTLFEHQALRLRMADLQARVDLLRYALHGIAEQGRLELRTAAAVKVTAARLGEE
VISECMHIFGGAGYLVDETTLGKWWRDMKLARVGGGTDEVLWELVAAGMTPDHDGYAAVVGASKA

Specific function: Catalyzes the dehydrogenation at the alpha-beta position of ACP-bound acyl chains. This results in the introduction of a double bond in the lipidic chain, which is further transferred to the epsilon-amino group of lysine residue in the mycobactin core by

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family

Homologues:

Organism=Homo sapiens, GI4501857, Length=373, Percent_Identity=25.4691689008043, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI226958412, Length=362, Percent_Identity=23.4806629834254, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226958414, Length=293, Percent_Identity=24.9146757679181, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI76496475, Length=345, Percent_Identity=23.4782608695652, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4557235, Length=345, Percent_Identity=23.4782608695652, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4557231, Length=281, Percent_Identity=24.5551601423488, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI187960098, Length=281, Percent_Identity=24.5551601423488, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4557233, Length=362, Percent_Identity=22.6519337016575, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4501859, Length=305, Percent_Identity=23.6065573770492, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI7656849, Length=354, Percent_Identity=23.4463276836158, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI87081958, Length=300, Percent_Identity=26, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786223, Length=246, Percent_Identity=23.5772357723577, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17551932, Length=365, Percent_Identity=27.3972602739726, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17505929, Length=366, Percent_Identity=26.775956284153, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17538396, Length=309, Percent_Identity=24.5954692556634, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506239, Length=297, Percent_Identity=25.9259259259259, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17533517, Length=294, Percent_Identity=22.4489795918367, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17570075, Length=302, Percent_Identity=23.5099337748344, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508101, Length=276, Percent_Identity=24.6376811594203, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17569725, Length=299, Percent_Identity=24.7491638795987, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21355753, Length=389, Percent_Identity=24.6786632390745, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666513, Length=301, Percent_Identity=22.9235880398671, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281363737, Length=310, Percent_Identity=24.5161290322581, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356377, Length=349, Percent_Identity=22.6361031518625, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660351, Length=375, Percent_Identity=22.9333333333333, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646207, Length=342, Percent_Identity=21.9298245614035, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBTN_MYCBO (P63432)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855035.1
- ProteinModelPortal:   P63432
- EnsemblBacteria:   EBMYCT00000017438
- GeneID:   1090672
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1381
- GeneTree:   EBGT00050000015286
- HOGENOM:   HBG564726
- OMA:   ICERAIA
- ProtClustDB:   CLSK791110
- BioCyc:   MBOV233413:MB1381-MONOMER
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100
- Gene3D:   G3DSA:2.40.110.10
- Gene3D:   G3DSA:1.10.540.10
- Gene3D:   G3DSA:1.20.140.10

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N; SSF56645 AcylCoA_dehyd_NM; SSF47203 AcylCoADH_C_like

EC number: NA

Molecular weight: Translated: 41181; Mature: 41049

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00072 ACYL_COA_DH_1; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGSDLDDFRGLLAKAFDERVVAWTAEAEAQERFPRQLIEHLGVCGVFDAKWATDARPD
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VGKLVELAFALGQLASAGIGVGVSLHDSAIAILRRFGKSDYLRDICDQAIRGAAVLCIGA
HHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEC
SEESGGSDLQIVETEIRSRDGGFEVRGVKKFVSLSPIADHIMVVARSVDHDPTSRHGNVA
CCCCCCCCEEEEHHHHHCCCCCEEEHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEE
VVAVPAAQVSVQTPYRKVGAGPLDTAAVCIDTWVPADALVARAGTGLAAISWGLAHERMS
EEEECCCEEEECCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
IAGQIAASCQRAIGITLARMMSRRQFGQTLFEHQALRLRMADLQARVDLLRYALHGIAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRLELRTAAAVKVTAARLGEEVISECMHIFGGAGYLVDETTLGKWWRDMKLARVGGGTDE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
VLWELVAAGMTPDHDGYAAVVGASKA
HHHHHHHCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
TAGSDLDDFRGLLAKAFDERVVAWTAEAEAQERFPRQLIEHLGVCGVFDAKWATDARPD
CCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VGKLVELAFALGQLASAGIGVGVSLHDSAIAILRRFGKSDYLRDICDQAIRGAAVLCIGA
HHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEC
SEESGGSDLQIVETEIRSRDGGFEVRGVKKFVSLSPIADHIMVVARSVDHDPTSRHGNVA
CCCCCCCCEEEEHHHHHCCCCCEEEHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEE
VVAVPAAQVSVQTPYRKVGAGPLDTAAVCIDTWVPADALVARAGTGLAAISWGLAHERMS
EEEECCCEEEECCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
IAGQIAASCQRAIGITLARMMSRRQFGQTLFEHQALRLRMADLQARVDLLRYALHGIAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRLELRTAAAVKVTAARLGEEVISECMHIFGGAGYLVDETTLGKWWRDMKLARVGGGTDE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
VLWELVAAGMTPDHDGYAAVVGASKA
HHHHHHHCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972