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The map label for this gene is fadD33

Identifier: 121637276

GI number: 121637276

Start: 1535795

End: 1537360

Strand: Direct

Name: fadD33

Synonym: BCG_1407

Alternate gene names: 121637276

Gene position: 1535795-1537360 (Clockwise)

Preceding gene: 121637275

Following gene: 121637277

Centisome position: 35.11

GC content: 67.24

Gene sequence:

>1566_bases
ATGAGTGAGCTCGCGGCCGTGCTCACGCGGTCCATGCAGGCCTCTGCCGGCGACTTGATGGTCCTCGACCGCGAGACCTC
GCTGTGGTGTCGGCACCCGTGGCCCGAGGTACACGGGCTGGCCGAGAGCGTAGCGGCCTGGCTGCTAGACCATGACCGAC
CCGCCGCGGTGGGTCTGGTCGGCGAACCGACGGTCGAGTTGGTCGCCGCGATCCAGGGTGCCTGGCTTGCCGGCGCTGCC
GTGTCGATCCTGCCCGGGCCGGTACGTGGCGCCAATGACCAGCGATGGGCGGACGCGACGTTGACCCGTTTCCTCGGGAT
TGGGGTGCGCACCGTATTGAGCCAGGGTTCCTACCTTGCCCGCCTGCGATCGGTCGATACGGCCGGCGTAACGATCGGAG
ATCTCAGCACGGCGGCGCACACCAATCGTTCGGCCACACCGGTGGCGAGTGAAGGGCCCGCGGTCCTTCAAGGTACCGCG
GGATCGACGGGCGCGCCCCGTACCGCCATCCTTTCGCCGGGCGCGGTGCTCAGCAACTTGCGTGGGCTCAATCAGCGCGT
GGGCACCGATGCTGCGACCGACGTCGGTTGCTCATGGTTACCGCTGTACCACGACATGGGGCTCGCTTTCGTGCTCTCTG
CTGCGCTGGCCGGTGCGCCGCTCTGGTTGGCCCCGACGACGGCGTTCACGGCGTCGCCGTTCCGTTGGTTGAGTTGGCTC
TCGGACAGTGGTGCCACCATGACCGCGGCACCGAACTTCGCCTACAACCTCATCGGCAAATACGCCAGGCGGGTATCCGA
GGTCGACCTGGGTGCCCTGCGAGTGACGCTCAACGGTGGAGAGCCGGTTGACTGCGATGGGCTGACGCGGTTCGCGGAGG
CGATGGCACCGTTCGGATTCGATGCCGGCGCCGTGTTGCCCTCCTACGGGCTCGCCGAGTCGACGTGCGCGGTGACCGTG
CCGGTCCCCGGAATTGGGTTGCTTGCCGACCGTGTCATCGACGGCAGCGGTGCGCATAAGCACGCGGTCCTGGGTAACCC
CATCCCCGGTATGGAGGTACGGATCTCGTGCGGTGATCAGGCGGCAGGCAATGCGAGCCGTGAAATTGGCGAAATCGAGA
TTCGCGGTGCGTCGATGATGGCGGGTTACCTGGGTCAGCAGCCGATCGACCCTGACGATTGGTTTGCCACCGGCGACCTC
GGCTATCTTGGCGCTGGCGGCCTGGTGGTGTGTGGTCGCGCGAAGGAAGTCATCTCCATCGCGGGACGCAACATCTTTCC
GACGGAGGTCGAGCTGGTGGCAGCGCAAGTTCGCGGAGTGCGCGAAGGCGCCGTGGTCGCCTTGGGCACCGGTGATCGCT
CGACCCGCCCCGGTCTGGTGGTCGCGGCCGAGTTCCGCGGCCCAGACGAGGCGAACGCCCGCGCCGAACTGATCCAACGC
GTTGCGTCCGAGTGCGGTATCGTCCCGTCCGACGTCGTCTTCGTGTCGCCTGGATCACTGCCCCGGACGTCGTCTGGAAA
ACTGCGCCGCTTGGCAGTCCGGCGCTCCCTGGAGATGGCGGACTGA

Upstream 100 bases:

>100_bases
GCCATCGAGGAGCGGCTCGGAGTCGCACTGTCCGAAGAGGAGCTCTTGACGTGCGACACGGTCGGAGAACTGGAGGCAGC
GATCGCGGCCAAATACCGCG

Downstream 100 bases:

>100_bases
TGACGGCCGGCTCCGACCTCGACGACTTCCGCGGTTTGCTCGCCAAAGCGTTCGACGAGCGGGTGGTGGCATGGACCGCA
GAAGCGGAAGCGCAGGAACG

Product: long-chain-fatty-acid--[acyl-carrier-protein] ligase

Products: NA

Alternate protein names: Fatty acyl-[acyl-carrier-protein] synthetase; Fatty acyl-ACP synthetase; Mycobactin synthetase protein M

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSELAAVLTRSMQASAGDLMVLDRETSLWCRHPWPEVHGLAESVAAWLLDHDRPAAVGLVGEPTVELVAAIQGAWLAGAA
VSILPGPVRGANDQRWADATLTRFLGIGVRTVLSQGSYLARLRSVDTAGVTIGDLSTAAHTNRSATPVASEGPAVLQGTA
GSTGAPRTAILSPGAVLSNLRGLNQRVGTDAATDVGCSWLPLYHDMGLAFVLSAALAGAPLWLAPTTAFTASPFRWLSWL
SDSGATMTAAPNFAYNLIGKYARRVSEVDLGALRVTLNGGEPVDCDGLTRFAEAMAPFGFDAGAVLPSYGLAESTCAVTV
PVPGIGLLADRVIDGSGAHKHAVLGNPIPGMEVRISCGDQAAGNASREIGEIEIRGASMMAGYLGQQPIDPDDWFATGDL
GYLGAGGLVVCGRAKEVISIAGRNIFPTEVELVAAQVRGVREGAVVALGTGDRSTRPGLVVAAEFRGPDEANARAELIQR
VASECGIVPSDVVFVSPGSLPRTSSGKLRRLAVRRSLEMAD

Sequences:

>Translated_521_residues
MSELAAVLTRSMQASAGDLMVLDRETSLWCRHPWPEVHGLAESVAAWLLDHDRPAAVGLVGEPTVELVAAIQGAWLAGAA
VSILPGPVRGANDQRWADATLTRFLGIGVRTVLSQGSYLARLRSVDTAGVTIGDLSTAAHTNRSATPVASEGPAVLQGTA
GSTGAPRTAILSPGAVLSNLRGLNQRVGTDAATDVGCSWLPLYHDMGLAFVLSAALAGAPLWLAPTTAFTASPFRWLSWL
SDSGATMTAAPNFAYNLIGKYARRVSEVDLGALRVTLNGGEPVDCDGLTRFAEAMAPFGFDAGAVLPSYGLAESTCAVTV
PVPGIGLLADRVIDGSGAHKHAVLGNPIPGMEVRISCGDQAAGNASREIGEIEIRGASMMAGYLGQQPIDPDDWFATGDL
GYLGAGGLVVCGRAKEVISIAGRNIFPTEVELVAAQVRGVREGAVVALGTGDRSTRPGLVVAAEFRGPDEANARAELIQR
VASECGIVPSDVVFVSPGSLPRTSSGKLRRLAVRRSLEMAD
>Mature_520_residues
SELAAVLTRSMQASAGDLMVLDRETSLWCRHPWPEVHGLAESVAAWLLDHDRPAAVGLVGEPTVELVAAIQGAWLAGAAV
SILPGPVRGANDQRWADATLTRFLGIGVRTVLSQGSYLARLRSVDTAGVTIGDLSTAAHTNRSATPVASEGPAVLQGTAG
STGAPRTAILSPGAVLSNLRGLNQRVGTDAATDVGCSWLPLYHDMGLAFVLSAALAGAPLWLAPTTAFTASPFRWLSWLS
DSGATMTAAPNFAYNLIGKYARRVSEVDLGALRVTLNGGEPVDCDGLTRFAEAMAPFGFDAGAVLPSYGLAESTCAVTVP
VPGIGLLADRVIDGSGAHKHAVLGNPIPGMEVRISCGDQAAGNASREIGEIEIRGASMMAGYLGQQPIDPDDWFATGDLG
YLGAGGLVVCGRAKEVISIAGRNIFPTEVELVAAQVRGVREGAVVALGTGDRSTRPGLVVAAEFRGPDEANARAELIQRV
ASECGIVPSDVVFVSPGSLPRTSSGKLRRLAVRRSLEMAD

Specific function: Activates lipidic moieties required for mycobactin biosynthesis. Converts medium- to long-chain aliphatic fatty acids into acyl adenylate, which is further transferred on to the phosphopantetheine arm of the carrier protein MbtL

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Escherichia coli, GI1788107, Length=374, Percent_Identity=25.1336898395722, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=379, Percent_Identity=26.3852242744063, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=384, Percent_Identity=24.4791666666667, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=375, Percent_Identity=24, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=381, Percent_Identity=23.8845144356955, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18859661, Length=373, Percent_Identity=24.3967828418231, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355181, Length=374, Percent_Identity=22.9946524064171, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBTM_MYCBO (P0A4X9)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855034.1
- ProteinModelPortal:   P0A4X9
- EnsemblBacteria:   EBMYCT00000015431
- GeneID:   1090670
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1380
- GeneTree:   EBGT00050000015137
- HOGENOM:   HBG547964
- OMA:   AWLSESR
- ProtClustDB:   PRK05851
- BioCyc:   MBOV233413:MB1380-MONOMER
- BRENDA:   6.2.1.20
- InterPro:   IPR020845
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.20

Molecular weight: Translated: 53958; Mature: 53827

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELAAVLTRSMQASAGDLMVLDRETSLWCRHPWPEVHGLAESVAAWLLDHDRPAAVGLV
CHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEC
GEPTVELVAAIQGAWLAGAAVSILPGPVRGANDQRWADATLTRFLGIGVRTVLSQGSYLA
CCCHHHHHHHHHCCHHHCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRSVDTAGVTIGDLSTAAHTNRSATPVASEGPAVLQGTAGSTGAPRTAILSPGAVLSNL
HHHCCCCCCEEECCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCHHHHHHH
RGLNQRVGTDAATDVGCSWLPLYHDMGLAFVLSAALAGAPLWLAPTTAFTASPFRWLSWL
HHHHHHHCCCCHHHCCCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
SDSGATMTAAPNFAYNLIGKYARRVSEVDLGALRVTLNGGEPVDCDGLTRFAEAMAPFGF
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCC
DAGAVLPSYGLAESTCAVTVPVPGIGLLADRVIDGSGAHKHAVLGNPIPGMEVRISCGDQ
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEECCCC
AAGNASREIGEIEIRGASMMAGYLGQQPIDPDDWFATGDLGYLGAGGLVVCGRAKEVISI
CCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCEECCCCCEECCCCEEEECCHHHHHHH
AGRNIFPTEVELVAAQVRGVREGAVVALGTGDRSTRPGLVVAAEFRGPDEANARAELIQR
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
VASECGIVPSDVVFVSPGSLPRTSSGKLRRLAVRRSLEMAD
HHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SELAAVLTRSMQASAGDLMVLDRETSLWCRHPWPEVHGLAESVAAWLLDHDRPAAVGLV
HHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEC
GEPTVELVAAIQGAWLAGAAVSILPGPVRGANDQRWADATLTRFLGIGVRTVLSQGSYLA
CCCHHHHHHHHHCCHHHCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRSVDTAGVTIGDLSTAAHTNRSATPVASEGPAVLQGTAGSTGAPRTAILSPGAVLSNL
HHHCCCCCCEEECCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEECCHHHHHHH
RGLNQRVGTDAATDVGCSWLPLYHDMGLAFVLSAALAGAPLWLAPTTAFTASPFRWLSWL
HHHHHHHCCCCHHHCCCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
SDSGATMTAAPNFAYNLIGKYARRVSEVDLGALRVTLNGGEPVDCDGLTRFAEAMAPFGF
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCC
DAGAVLPSYGLAESTCAVTVPVPGIGLLADRVIDGSGAHKHAVLGNPIPGMEVRISCGDQ
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEECCCC
AAGNASREIGEIEIRGASMMAGYLGQQPIDPDDWFATGDLGYLGAGGLVVCGRAKEVISI
CCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCEECCCCCEECCCCEEEECCHHHHHHH
AGRNIFPTEVELVAAQVRGVREGAVVALGTGDRSTRPGLVVAAEFRGPDEANARAELIQR
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
VASECGIVPSDVVFVSPGSLPRTSSGKLRRLAVRRSLEMAD
HHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972