Click here to switch to the map view.

The map label for this gene is ybjS [C]

Identifier: 121637037

GI number: 121637037

Start: 1264259

End: 1265371

Strand: Reverse

Name: ybjS [C]

Synonym: BCG_1166c

Alternate gene names: 121637037

Gene position: 1265371-1264259 (Counterclockwise)

Preceding gene: 121637038

Following gene: 121637034

Centisome position: 28.93

GC content: 64.87

Gene sequence:

>1113_bases
ATGCTTCGCCGCATGGGTGATGCATCGCTGACAACCGAGCTCGGCCGCGTTCTGGTCACCGGCGGCGCGGGCTTCGTGGG
CGCCAACCTGGTGACCACCTTGCTGGACCGCGGGCACTGGGTGCGTTCCTTCGACCGCGCGCCGTCGCTGTTGCCTGCGC
ATCCGCAACTGGAGGTGCTGCAAGGGGACATCACCGACGCGGACGTCTGCGCCGCGGCCGTGGACGGCATCGACACGATC
TTCCACACCGCAGCGATCATCGAGCTGATGGGCGGCGCGTCGGTCACCGACGAGTACCGCCAACGTAGCTTTGCGGTCAA
CGTCGGCGGCACCGAGAACCTGCTGCACGCCGGCCAGCGGGCCGGGGTGCAGCGGTTCGTCTACACGTCATCCAACAGTG
TGGTGATGGGCGGCCAGAACATCGCCGGCGGTGACGAGACGCTGCCCTATACCGACCGGTTCAACGACCTCTACACCGAG
ACCAAGGTGGTTGCCGAGCGATTCGTGTTGGCCCAGAACGGTGTCGACGGCATGCTGACGTGCGCGATCCGGCCCAGCGG
CATCTGGGGAAACGGCGATCAGACGATGTTCCGCAAGCTGTTCGAAAGTGTGCTCAAGGGCCACGTCAAGGTGCTGGTCG
GGCGCAAGTCGGCCCGGCTGGATAACTCTTACGTGCACAACCTGATTCACGGTTTCATCTTGGCCGCTGCCCATCTGGTG
CCGGACGGCACAGCGCCCGGGCAGGCTTACTTCATCAACGACGCAGAGCCGATCAATATGTTCGAGTTCGCTCGGCCGGT
GCTCGAGGCGTGCGGGCAGCGCTGGCCGAAGATGCGGATTTCCGGCCCCGCGGTCCGCTGGGTAATGACGGGGTGGCAGC
GGCTGCACTTCCGGTTCGGATTCCCCGCGCCGCTGCTCGAGCCGCTGGCCGTCGAACGACTGTACCTGGACAACTACTTT
TCGATCGCTAAGGCACGCCGCGACCTGGGCTATGAGCCGCTGTTCACCACCCAGCAGGCGCTGACCGAATGCCTGCCGTA
CTACGTGAGTCTGTTTGAGCAGATGAAGAACGAGGCCCGGGCGGAAAAAACGGCCGCCACAGTCAAGCCGTAG

Upstream 100 bases:

>100_bases
GCAACTCGCCAAAAGGTGCGAGGAGCACTTAGCTGGTGCACGCCAGCGGGTGTCCGATGTGCTGGCCGGCGACGAGGCCC
AAAACGGCTAAGGCAAGGTT

Downstream 100 bases:

>100_bases
CCAGAATTATCTGAAACTCACCACTTGCTGCCCCAGGTCGCTCGGATGTGTGCGTCGACGTCGTGCACGACGGCGTCTCG
CCTGCCGATAATCAGGCAGG

Product: putative cholesterol dehydrogenase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVLQGDITDADVCAAAVDGIDTI
FHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTE
TKVVAERFVLAQNGVDGMLTCAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLV
PDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYF
SIAKARRDLGYEPLFTTQQALTECLPYYVSLFEQMKNEARAEKTAATVKP

Sequences:

>Translated_370_residues
MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVLQGDITDADVCAAAVDGIDTI
FHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTE
TKVVAERFVLAQNGVDGMLTCAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLV
PDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYF
SIAKARRDLGYEPLFTTQQALTECLPYYVSLFEQMKNEARAEKTAATVKP
>Mature_370_residues
MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVLQGDITDADVCAAAVDGIDTI
FHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTE
TKVVAERFVLAQNGVDGMLTCAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLV
PDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYF
SIAKARRDLGYEPLFTTQQALTECLPYYVSLFEQMKNEARAEKTAATVKP

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family

Homologues:

Organism=Homo sapiens, GI193211614, Length=335, Percent_Identity=32.5373134328358, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI8393516, Length=335, Percent_Identity=32.5373134328358, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI116268111, Length=341, Percent_Identity=35.7771260997067, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI310132178, Length=332, Percent_Identity=32.8313253012048, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI310113012, Length=332, Percent_Identity=32.8313253012048, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI239745448, Length=332, Percent_Identity=32.8313253012048, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI260763931, Length=376, Percent_Identity=29.5212765957447, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4504509, Length=376, Percent_Identity=29.5212765957447, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4504507, Length=378, Percent_Identity=29.8941798941799, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI19923621, Length=373, Percent_Identity=32.9758713136729, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI218563686, Length=209, Percent_Identity=33.9712918660287, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI218563684, Length=209, Percent_Identity=33.9712918660287, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87081792, Length=335, Percent_Identity=23.5820895522388, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17570557, Length=369, Percent_Identity=29.5392953929539, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17509709, Length=372, Percent_Identity=29.5698924731183, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI193211272, Length=334, Percent_Identity=27.5449101796407, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321437, Length=361, Percent_Identity=28.808864265928, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): 3BHS_MYCTU (O53454)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842575
- PIR:   H70897
- RefSeq:   NP_215622.1
- RefSeq:   NP_335580.1
- ProteinModelPortal:   O53454
- EnsemblBacteria:   EBMYCT00000002215
- EnsemblBacteria:   EBMYCT00000071008
- GeneID:   886004
- GeneID:   924962
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1137
- KEGG:   mtu:Rv1106c
- TIGR:   MT1137
- TubercuList:   Rv1106c
- GeneTree:   EBGT00050000015184
- HOGENOM:   HBG755066
- OMA:   TKFIQAD
- ProtClustDB:   CLSK790953
- GO:   GO:0005829
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1

Molecular weight: Translated: 40742; Mature: 40742

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: ACT_SITE 158-158 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVL
CCCCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHH
QGDITDADVCAAAVDGIDTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCHHHHHHHHHH
AGVQRFVYTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGVDGMLT
HCCEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
CAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLV
EEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHCCHHHHHHHHHHHHHHHHHHC
PDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMTGWQRLHFRFG
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEECC
FPAPLLEPLAVERLYLDNYFSIAKARRDLGYEPLFTTQQALTECLPYYVSLFEQMKNEAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKTAATVKP
HHHHHCCCCC
>Mature Secondary Structure
MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVL
CCCCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHH
QGDITDADVCAAAVDGIDTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCHHHHHHHHHH
AGVQRFVYTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGVDGMLT
HCCEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
CAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLV
EEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHCCHHHHHHHHHHHHHHHHHHC
PDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMTGWQRLHFRFG
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEECC
FPAPLLEPLAVERLYLDNYFSIAKARRDLGYEPLFTTQQALTECLPYYVSLFEQMKNEAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKTAATVKP
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036