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The map label for this gene is purF

Identifier: 121636732

GI number: 121636732

Start: 932533

End: 934116

Strand: Direct

Name: purF

Synonym: BCG_0860

Alternate gene names: 121636732

Gene position: 932533-934116 (Clockwise)

Preceding gene: 121636731

Following gene: 121636733

Centisome position: 21.32

GC content: 67.11

Gene sequence:

>1584_bases
GTGGCCGTAGACTCGGATTACGTCACCGACCGCGCCGCAGGGAGCCGCCAAACCGTGACCGGCCAGCAACCCGAGCAAGA
CCTGAACTCGCCCCGGGAAGAGTGCGGTGTCTTCGGGGTCTGGGCCCCGGGTGAAGACGTCGCCAAACTCACCTACTACG
GCCTGTACGCGTTGCAGCATCGCGGCCAGGAAGCCGCCGGGATCGCCGTCGCCGACGGCTCCCAGGTGCTGGTCTTCAAA
GACCTCGGCCTGGTCAGCCAGGTGTTCGACGAGCAGACGTTGGCGGCCATGCAGGGCCATGTCGCCATCGGGCACTGTCG
TTACTCCACCACCGGGGACACGACGTGGGAGAACGCCCAGCCCGTGTTCCGCAACACCGCCGCTGGCACCGGTGTTGCGT
TGGGCCACAACGGAAATCTGGTCAATGCCGCTGCCCTTGCCGCCCGCGCCCGCGACGCGGGGTTGATCGCCACCCGCTGC
CCAGCCCCGGCGACGACGGACTCCGACATTCTGGGGGCGCTGCTGGCCCACGGTGCTGCCGATTCCACCCTCGAACAGGC
GGCGCTGGACCTGCTGCCCACAGTGCGGGGAGCGTTCTGTCTGACGTTCATGGACGAAAACACGCTTTATGCGTGCCGCG
ACCCGTACGGGGTGCGCCCGCTATCGCTCGGGCGTTTGGACCGTGGCTGGGTGGTGGCCTCCGAAACGGCCGCACTCGAC
ATCGTCGGCGCCTCGTTCGTCCGTGATATCGAACCGGGCGAATTGCTGGCTATCGACGCCGACGGGGTGCGGTCCACCCG
CTTTGCCAACCCCACGCCCAAGGGCTGCGTATTCGAATACGTCTACCTGGCGCGGCCGGACAGTACGATCGCCGGCCGGT
CGGTACACGCCGCGCGGGTGGAGATCGGTCGCCGACTGGCTCGGGAATGCCCGGTCGAGGCCGACTTGGTGATTGGTGTG
CCGGAATCCGGCACACCCGCCGCGGTCGGCTACGCGCAGGAGTCCGGCGTTCCATATGGGCAGGGTCTGATGAAGAACGC
CTATGTCGGGCGCACCTTCATCCAGCCGTCACAGACCATCCGTCAGCTCGGCATCCGGCTGAAGCTCAACCCGCTCAAAG
AGGTGATCCGCGGCAAGCGGCTCATCGTCGTCGACGACTCGATCGTGCGGGGCAACACCCAGCGTGCGCTGGTACGCATG
CTGCGCGAGGCCGGTGCGGTCGAATTGCATGTGCGCATCGCCTCGCCACCGGTGAAGTGGCCGTGCTTCTACGGTATCGA
CTTCCCCTCGCCGGCCGAGTTGATCGCCAACGCCGTGGAAAACGAGGACGAGATGCTGGAGGCGGTACGGCATGCCATCG
GGGCCGACACGCTGGGATACATCTCGCTGCGGGGCATGGTGGCGGCGTCCGAGCAGCCCACGTCGCGGCTGTGCACCGCC
TGCTTCGACGGCAAGTATCCAATAGAGCTGCCCCGCGAGACCGCGCTAGGCAAAAATGTCATCGAGCACATGCTCGCCAA
TGCGGCCCGCGGAGCCGCGCTGGGCGAACTCGCCGCCGACGACGAAGTCCCCGTTGGGCGCTGA

Upstream 100 bases:

>100_bases
TGGATCTCGGCTGATTCCGGCGTGCTGAGCTGCGGCTATGGTGTGTGAGGGTGGCGCCGGGGTGCCCGACACGTAAGCCG
AATTCGGCGGTGCAGACGTC

Downstream 100 bases:

>100_bases
CAAAACGCACGCGCGGTAGCCTTTATCGCGATGACGGATCTCGCAAAAGGCCCCGGAAAAGACCCGGGTAGTCGGGGTAT
CACCTACGCGTCGGCCGGGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MAVDSDYVTDRAAGSRQTVTGQQPEQDLNSPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFK
DLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALGHNGNLVNAAALAARARDAGLIATRC
PAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDPYGVRPLSLGRLDRGWVVASETAALD
IVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSVHAARVEIGRRLARECPVEADLVIGV
PESGTPAAVGYAQESGVPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALVRM
LREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANAVENEDEMLEAVRHAIGADTLGYISLRGMVAASEQPTSRLCTA
CFDGKYPIELPRETALGKNVIEHMLANAARGAALGELAADDEVPVGR

Sequences:

>Translated_527_residues
MAVDSDYVTDRAAGSRQTVTGQQPEQDLNSPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFK
DLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALGHNGNLVNAAALAARARDAGLIATRC
PAPATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDPYGVRPLSLGRLDRGWVVASETAALD
IVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSVHAARVEIGRRLARECPVEADLVIGV
PESGTPAAVGYAQESGVPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALVRM
LREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANAVENEDEMLEAVRHAIGADTLGYISLRGMVAASEQPTSRLCTA
CFDGKYPIELPRETALGKNVIEHMLANAARGAALGELAADDEVPVGR
>Mature_526_residues
AVDSDYVTDRAAGSRQTVTGQQPEQDLNSPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFKD
LGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQPVFRNTAAGTGVALGHNGNLVNAAALAARARDAGLIATRCP
APATTDSDILGALLAHGAADSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDPYGVRPLSLGRLDRGWVVASETAALDI
VGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSVHAARVEIGRRLARECPVEADLVIGVP
ESGTPAAVGYAQESGVPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALVRML
REAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANAVENEDEMLEAVRHAIGADTLGYISLRGMVAASEQPTSRLCTAC
FDGKYPIELPRETALGKNVIEHMLANAARGAALGELAADDEVPVGR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=40.8477842003854, Blast_Score=367, Evalue=1e-101,
Organism=Escherichia coli, GI1788651, Length=479, Percent_Identity=39.2484342379958, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1790167, Length=232, Percent_Identity=28.448275862069, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=491, Percent_Identity=39.1038696537678, Blast_Score=317, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=32.712215320911, Blast_Score=233, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24659598, Length=515, Percent_Identity=40.7766990291262, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI28573187, Length=517, Percent_Identity=41.5860735009671, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=41.4225941422594, Blast_Score=355, Evalue=4e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR1_MYCBO (P65830)

Other databases:

- EMBL:   BX248336
- RefSeq:   NP_854489.1
- ProteinModelPortal:   P65830
- SMR:   P65830
- MEROPS:   C44.001
- EnsemblBacteria:   EBMYCT00000015800
- GeneID:   1092560
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0831
- GeneTree:   EBGT00050000016850
- HOGENOM:   HBG392416
- OMA:   VWAPGEE
- ProtClustDB:   PRK07847
- BioCyc:   MBOV233413:MB0831-MONOMER
- BRENDA:   2.4.2.14
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836
- PANTHER:   PTHR11907
- PIRSF:   PIRSF000485
- TIGRFAMs:   TIGR01134

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran

EC number: =2.4.2.14

Molecular weight: Translated: 56162; Mature: 56031

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS51278 GATASE_TYPE_2; PS00103 PUR_PYR_PR_TRANSFER

Important sites: ACT_SITE 35-35

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVDSDYVTDRAAGSRQTVTGQQPEQDLNSPREECGVFGVWAPGEDVAKLTYYGLYALQH
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHH
RGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQ
CCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
PVFRNTAAGTGVALGHNGNLVNAAALAARARDAGLIATRCPAPATTDSDILGALLAHGAA
CHHHCCCCCCCEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCC
DSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDPYGVRPLSLGRLDRGWVVASETAALD
CHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCEEE
IVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSVHAARV
EECHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
EIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGVPYGQGLMKNAYVGRTFIQPSQTI
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHCCCCCCCHHHHCCCCCCEECCHHHHH
RQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALVRMLREAGAVELHVRIASPPVKW
HHCCCEEEECHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCC
PCFYGIDFPSPAELIANAVENEDEMLEAVRHAIGADTLGYISLRGMVAASEQPTSRLCTA
CEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCHHHHHHH
CFDGKYPIELPRETALGKNVIEHMLANAARGAALGELAADDEVPVGR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AVDSDYVTDRAAGSRQTVTGQQPEQDLNSPREECGVFGVWAPGEDVAKLTYYGLYALQH
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHH
RGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMQGHVAIGHCRYSTTGDTTWENAQ
CCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
PVFRNTAAGTGVALGHNGNLVNAAALAARARDAGLIATRCPAPATTDSDILGALLAHGAA
CHHHCCCCCCCEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCC
DSTLEQAALDLLPTVRGAFCLTFMDENTLYACRDPYGVRPLSLGRLDRGWVVASETAALD
CHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCEEE
IVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTIAGRSVHAARV
EECHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
EIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGVPYGQGLMKNAYVGRTFIQPSQTI
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHCCCCCCCHHHHCCCCCCEECCHHHHH
RQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALVRMLREAGAVELHVRIASPPVKW
HHCCCEEEECHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCC
PCFYGIDFPSPAELIANAVENEDEMLEAVRHAIGADTLGYISLRGMVAASEQPTSRLCTA
CEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCHHHHHHH
CFDGKYPIELPRETALGKNVIEHMLANAARGAALGELAADDEVPVGR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972