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The map label for this gene is entA [C]

Identifier: 121636353

GI number: 121636353

Start: 558194

End: 559129

Strand: Reverse

Name: entA [C]

Synonym: BCG_0478c

Alternate gene names: 121636353

Gene position: 559129-558194 (Counterclockwise)

Preceding gene: 121636355

Following gene: 121636352

Centisome position: 12.78

GC content: 65.28

Gene sequence:

>936_bases
ATGACCGCCAACGACAACAAGACCCGTAAATGGTCGGCCGCAGACGTCCCCGATCAAAGCGGGCGCGTCGTTGTGGTCAC
CGGCGCCAACACCGGCATCGGCTACCACACCGCCGCCGTGTTTGCCGACCGCGGTGCACACGTAGTGTTGGCCGTCCGCA
ATCTCGAGAAGGGCAACGCCGCCCGGGCCCGCATCATGGCCGCCCGCCCAGGCGCCCACGTCACGCTGCAGCAACTCGAT
CTGTGCTCGCTGGACTCGGTGCGCGCAGCCGCCGACGCGTTACGCACGGCCTATCCGCGCATCGACGTGCTAATCAATAA
CGCCGGCGTGATGTGGACGCCGAAGCAGGTCACCAAGGACGGTTTCGAGCTGCAGTTCGGTACCAACCATCTCGGTCATT
TCGCACTAACCGGACTGGTACTCGACCACATGCTGCCGGTACCCGGTTCGCGGGTGGTGACCGTCAGCAGCCAGGGCCAC
CGGATTCACGCTGCCATCCACTTCGACGACTTGCAGTGGGAACGCCGTTACAACCGCGTCGCCGCCTACGGACAAGCCAA
ACTGGCTAATCTGCTGTTCACCTACGAGCTGCAACGCCGGCTGGGCGAAGCGGGCAAATCCACCATCGCCGTCGCCGCTC
ACCCTGGCGGCTCCAACACCGAGCTGACTCGCAACCTGCCGCGACTTATCCGGCCCGTCGCTACCGTGCTCGGGCCGTTG
CTTTTCCAAAGCCCAGAGATGGGCGCCCTGCCAACACTGCGTGCCGCCACCGATCCGACCACGCAGGGCGGGCAATACTA
CGGCCCGGACGGGTTCGGCGAGCAGCGCGGTCACCCGAAGGTGGTCCAATCCAGCGCGCAGTCCCACGACAAAGATCTGC
AGCGCCGCCTCTGGACCGTCTCCGAAGAACTCACCGGCGTCAGCTTCGGAGTCTGA

Upstream 100 bases:

>100_bases
TAGCACTCGCCTATGCCGAGTGCAAGAAGCCCCGCACCGGGTCATGCCCCTCGTTCGACCTTGTCCTCGGCCCTCCGATC
CGGGTGAGTATGTTCGGCCC

Downstream 100 bases:

>100_bases
ACGACCGATAATGGCCGGGTGCGGTCAGTCCAGGAGCATCAACGTGTCGTAGCGGAGATGATCCGCGCCTGTCGCCCGAT
TACGGTTCCGCTGACCCAGG

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTANDNKTRKWSAADVPDQSGRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQQLD
LCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALTGLVLDHMLPVPGSRVVTVSSQGH
RIHAAIHFDDLQWERRYNRVAAYGQAKLANLLFTYELQRRLGEAGKSTIAVAAHPGGSNTELTRNLPRLIRPVATVLGPL
LFQSPEMGALPTLRAATDPTTQGGQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTVSEELTGVSFGV

Sequences:

>Translated_311_residues
MTANDNKTRKWSAADVPDQSGRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQQLD
LCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALTGLVLDHMLPVPGSRVVTVSSQGH
RIHAAIHFDDLQWERRYNRVAAYGQAKLANLLFTYELQRRLGEAGKSTIAVAAHPGGSNTELTRNLPRLIRPVATVLGPL
LFQSPEMGALPTLRAATDPTTQGGQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTVSEELTGVSFGV
>Mature_310_residues
TANDNKTRKWSAADVPDQSGRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQQLDL
CSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALTGLVLDHMLPVPGSRVVTVSSQGHR
IHAAIHFDDLQWERRYNRVAAYGQAKLANLLFTYELQRRLGEAGKSTIAVAAHPGGSNTELTRNLPRLIRPVATVLGPLL
FQSPEMGALPTLRAATDPTTQGGQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTVSEELTGVSFGV

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=303, Percent_Identity=43.2343234323432, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI166795268, Length=287, Percent_Identity=41.8118466898955, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI10190746, Length=304, Percent_Identity=37.171052631579, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI193804850, Length=297, Percent_Identity=38.7205387205387, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI186928839, Length=289, Percent_Identity=40.4844290657439, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI19923931, Length=261, Percent_Identity=41.7624521072797, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI7706523, Length=304, Percent_Identity=35.5263157894737, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI146231950, Length=298, Percent_Identity=37.248322147651, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI7705421, Length=239, Percent_Identity=27.6150627615063, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17532805, Length=318, Percent_Identity=32.3899371069182, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17558006, Length=298, Percent_Identity=32.2147651006711, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI193207620, Length=307, Percent_Identity=31.5960912052117, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17532791, Length=307, Percent_Identity=30.9446254071661, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71992402, Length=299, Percent_Identity=33.7792642140468, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17535059, Length=299, Percent_Identity=33.7792642140468, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI72001364, Length=300, Percent_Identity=31, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17505416, Length=266, Percent_Identity=29.6992481203008, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32565532, Length=210, Percent_Identity=30.4761904761905, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533174, Length=293, Percent_Identity=27.6450511945392, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533172, Length=293, Percent_Identity=26.962457337884, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324820, Length=323, Percent_Identity=28.7925696594427, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573296, Length=297, Percent_Identity=42.4242424242424, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI19921754, Length=297, Percent_Identity=42.0875420875421, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI22024069, Length=296, Percent_Identity=41.8918918918919, Blast_Score=191, Evalue=8e-49,
Organism=Drosophila melanogaster, GI161076371, Length=256, Percent_Identity=42.1875, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24586328, Length=294, Percent_Identity=38.7755102040816, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45554248, Length=252, Percent_Identity=42.4603174603175, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24640117, Length=252, Percent_Identity=42.4603174603175, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553409, Length=253, Percent_Identity=35.1778656126482, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24647948, Length=253, Percent_Identity=35.1778656126482, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24647946, Length=253, Percent_Identity=35.1778656126482, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI20129347, Length=298, Percent_Identity=34.5637583892617, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656154, Length=264, Percent_Identity=36.3636363636364, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922628, Length=264, Percent_Identity=36.3636363636364, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647931, Length=322, Percent_Identity=32.6086956521739, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21357041, Length=211, Percent_Identity=30.3317535545024, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 33651; Mature: 33519

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANDNKTRKWSAADVPDQSGRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNA
CCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCHHHEEEEECCCCEEEEEEECCCCCCC
ARARIMAARPGAHVTLQQLDLCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKD
CCEEEEEECCCCEEEHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCEEECCHHHCCC
GFELQFGTNHLGHFALTGLVLDHMLPVPGSRVVTVSSQGHRIHAAIHFDDLQWERRYNRV
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHH
AAYGQAKLANLLFTYELQRRLGEAGKSTIAVAAHPGGSNTELTRNLPRLIRPVATVLGPL
HHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LFQSPEMGALPTLRAATDPTTQGGQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTV
HHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SEELTGVSFGV
HHHHCCCCCCC
>Mature Secondary Structure 
TANDNKTRKWSAADVPDQSGRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNA
CCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCHHHEEEEECCCCEEEEEEECCCCCCC
ARARIMAARPGAHVTLQQLDLCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKD
CCEEEEEECCCCEEEHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCEEECCHHHCCC
GFELQFGTNHLGHFALTGLVLDHMLPVPGSRVVTVSSQGHRIHAAIHFDDLQWERRYNRV
CEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHH
AAYGQAKLANLLFTYELQRRLGEAGKSTIAVAAHPGGSNTELTRNLPRLIRPVATVLGPL
HHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LFQSPEMGALPTLRAATDPTTQGGQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTV
HHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SEELTGVSFGV
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]