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The map label for this gene is def [H]

Identifier: 121636343

GI number: 121636343

Start: 548618

End: 549211

Strand: Reverse

Name: def [H]

Synonym: BCG_0468c

Alternate gene names: 121636343

Gene position: 549211-548618 (Counterclockwise)

Preceding gene: 121636349

Following gene: 121636342

Centisome position: 12.55

GC content: 65.66

Gene sequence:

>594_bases
ATGGCAGTCGTACCCATCCGCATCGTGGGCGATCCCGTCTTACACACTGCGACCACACCGGTGACGGTCGCCGCCGACGG
TTCACTCCCGGCGGATCTCGCCCAGTTGATCGCCACCATGTACGACACCATGGACGCCGCCAACGGAGTCGGCCTGGCTG
CCAACCAGATCGGCTGCAGCCTGCGGCTCTTCGTCTACGATTGCGCCGCGGACCGCGCAATGACCGCCCGCCGACGCGGT
GTGGTCATCAATCCGGTGCTTGAGACCTCCGAAATACCTGAGACCATGCCCGACCCGGACACCGACGACGAAGGCTGTCT
GTCGGTTCCCGGCGAGTCATTTCCTACCGGACGCGCGAAGTGGGCACGAGTCACCGGACTCGACGCCGATGGCAGTCCGG
TCAGTATCGAGGGCACCGGCCTGTTCGCGCGGATGCTGCAGCACGAAACCGGGCACCTTGATGGATTCCTGTACCTGGAC
CGCCTCATCGGCCGGTACGCCCGCAACGCCAAACGGGCCGTCAAGTCACATGGCTGGGGCGTTCCCGGACTGTCGTGGCT
GCCCGGCGAGGACCCCGACCCGTTCGGTCACTAA

Upstream 100 bases:

>100_bases
GATGATGCCCGCCGCCCCGCGGCGCGCCTAAGCCGATTGCCCGCCTCCTCAGCCCGCCGCCTCGCGGCGCGTATCGTCGG
CGCGCTTAAGCTAGCCCACC

Downstream 100 bases:

>100_bases
TGGTCTCGTGGCCGGGGCTTGGGACACGGGTAACGGTGCGCTACCGCCGGCCCGCCGGATCGATGCCACCGCTGACCGAT
GCGGTGGGTCGGCTGCTAGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MAVVPIRIVGDPVLHTATTPVTVAADGSLPADLAQLIATMYDTMDAANGVGLAANQIGCSLRLFVYDCAADRAMTARRRG
VVINPVLETSEIPETMPDPDTDDEGCLSVPGESFPTGRAKWARVTGLDADGSPVSIEGTGLFARMLQHETGHLDGFLYLD
RLIGRYARNAKRAVKSHGWGVPGLSWLPGEDPDPFGH

Sequences:

>Translated_197_residues
MAVVPIRIVGDPVLHTATTPVTVAADGSLPADLAQLIATMYDTMDAANGVGLAANQIGCSLRLFVYDCAADRAMTARRRG
VVINPVLETSEIPETMPDPDTDDEGCLSVPGESFPTGRAKWARVTGLDADGSPVSIEGTGLFARMLQHETGHLDGFLYLD
RLIGRYARNAKRAVKSHGWGVPGLSWLPGEDPDPFGH
>Mature_196_residues
AVVPIRIVGDPVLHTATTPVTVAADGSLPADLAQLIATMYDTMDAANGVGLAANQIGCSLRLFVYDCAADRAMTARRRGV
VINPVLETSEIPETMPDPDTDDEGCLSVPGESFPTGRAKWARVTGLDADGSPVSIEGTGLFARMLQHETGHLDGFLYLDR
LIGRYARNAKRAVKSHGWGVPGLSWLPGEDPDPFGH

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=163, Percent_Identity=34.9693251533742, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645728, Length=168, Percent_Identity=31.547619047619, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24645726, Length=166, Percent_Identity=30.1204819277108, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20939; Mature: 20808

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVPIRIVGDPVLHTATTPVTVAADGSLPADLAQLIATMYDTMDAANGVGLAANQIGCS
CCEEEEEECCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEHHCCCCE
LRLFVYDCAADRAMTARRRGVVINPVLETSEIPETMPDPDTDDEGCLSVPGESFPTGRAK
EEEEEEECCCHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
WARVTGLDADGSPVSIEGTGLFARMLQHETGHLDGFLYLDRLIGRYARNAKRAVKSHGWG
EEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPGLSWLPGEDPDPFGH
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVVPIRIVGDPVLHTATTPVTVAADGSLPADLAQLIATMYDTMDAANGVGLAANQIGCS
CEEEEEECCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEHHCCCCE
LRLFVYDCAADRAMTARRRGVVINPVLETSEIPETMPDPDTDDEGCLSVPGESFPTGRAK
EEEEEEECCCHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
WARVTGLDADGSPVSIEGTGLFARMLQHETGHLDGFLYLDRLIGRYARNAKRAVKSHGWG
EEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPGLSWLPGEDPDPFGH
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]