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The map label for this gene is thiC

Identifier: 121636337

GI number: 121636337

Start: 539397

End: 541040

Strand: Reverse

Name: thiC

Synonym: BCG_0462c

Alternate gene names: 121636337

Gene position: 541040-539397 (Counterclockwise)

Preceding gene: 121636338

Following gene: 121636336

Centisome position: 12.37

GC content: 64.96

Gene sequence:

>1644_bases
ATGACCATCACCGTTGAACCGTCGGTGACCACGGGTCCCATCGCGGGCAGCGCCAAGGCCTACCGTGAAATCGAGGCTCC
CGGCAGCGGAGCTACTCTCCAAGTCCCGTTTCGACGGGTGCACTTGTCCACCGGAGACCACTTCGACCTCTACGACACCT
CCGGGCCCTACACCGACACGGACACGGTGATCGACCTGACCGCGGGGCTGCCGCATAGGCCCGGAGTGGTTCGCGATCGG
GGCACCCAGCTGCAGCGGGCCCGCGCCGGGGAGATCACCGCCGAGATGGCGTTCATCGCCGCCCGCGAAGACATGTCCGC
CGAGCTAGTGCGCGACGAGGTCGCCCGCGGCCGCGCGGTGATCCCGGCCAACCACCACCACCCCGAGAGCGAGCCGATGA
TCATCGGCAAGGCGTTCGCGGTGAAAGTCAACGCCAACATCGGCAACTCGGCGGTGACGAGCTCGATCGCCGAGGAGGTC
GACAAGATGGTGTGGGCCACCCGCTGGGGGGCCGACACCATCATGGACCTGTCCACCGGCAAGAACATCCACGAAACCCG
CGAGTGGATCCTGCGCAATTCTCCCGTGCCGGTCGGCACCGTGCCGATCTATCAGGCGCTGGAGAAAGTCAAGGGCGATC
CGACCGAGCTGACCTGGGAGATCTACCGCGACACCGTGATCGAGCAGTGTGAGCAAGGCGTGGACTACATGACGGTGCAC
GCCGGGGTGCTGCTGCGGTATGTGCCGCTGACCGCCAAGCGGGTCACCGGCATCGTGTCCCGCGGGGGTTCGATCATGGC
CGCGTGGTGTTTGGCACATCATCGGGAGTCGTTCTTGTACACCAACTTTGAGGAGCTCTGCGATATTTTCGCCCGCTACG
ACGTCACCTTCTCACTCGGTGACGGGCTGCGACCAGGGTCGATCGCTGATGCCAACGACGCCGCGCAGTTCGCCGAGCTG
CGCACCCTGGGCGAGCTCACCAAGATCGCCAAAGCCCATGGCGCACAGGTGATGATCGAGGGGCCGGGCCATATCCCAAT
GCACAAGATCGTCGAGAATGTGCGGCTGGAAGAGGAACTGTGTGAGGAGGCCCCGTTCTACACGCTGGGTCCGCTGGCCA
CCGACATCGCGCCGGCCTACGACCACATCACCTCGGCGATCGGTGCGGCCATCATCGCCCAAGCCGGTACCGCGATGCTG
TGCTACGTCACCCCCAAGGAGCACCTCGGGTTGCCGGACCGCAAGGACGTCAAGGACGGGGTGATCGCCTACAAGATCGC
CGCGCATGCGGCCGATTTGGCCAAGGGCCATCCGCGCGCCCAGGAGCGCGACGACGCTTTGAGCACGGCGCGTTTCGAGT
TCCGCTGGAACGACCAGTTCGCACTGTCGCTGGATCCCGACACCGCACGGGAATTCCACGACGAAACCCTGCCGGCGGAG
CCGGCCAAGACCGCGCACTTCTGCTCGATGTGCGGACCGAAGTTCTGCTCCATGCGCATCACCCAGGACGTCCGTGAGTA
CGCCGCCGAACACGGGCTTGAGACCGAAGCGGACATCGAAGCCGTGCTCGCCGCCGGAATGGCCGAAAAGTCACGTGAAT
TCGCAGAGCACGGCAATCGGGTGTATCTCCCGATAACCCAGTGA

Upstream 100 bases:

>100_bases
GGTACCCACGCGGGAGCGCACGCCGAGTGCGCTGAGAGGACGGCTCGGGGCCGTCGACCGTACGAACCTGACCGGGTAAT
GCCGGCGTAGGGAGTTGCAA

Downstream 100 bases:

>100_bases
ACTACCTCCCGCTGGCACCGCCGGGAATGACGCCGCCGCGGGTGTTGAGCATCGCCGGATCGGACTCCGGGGGCGGTGCC
GGCATCCAAGCCGATATGCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTITVEPSVTTGPIAGSAKAYREIEAPGSGATLQVPFRRVHLSTGDHFDLYDTSGPYTDTDTVIDLTAGLPHRPGVVRDR
GTQLQRARAGEITAEMAFIAAREDMSAELVRDEVARGRAVIPANHHHPESEPMIIGKAFAVKVNANIGNSAVTSSIAEEV
DKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTELTWEIYRDTVIEQCEQGVDYMTVH
AGVLLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFEELCDIFARYDVTFSLGDGLRPGSIADANDAAQFAEL
RTLGELTKIAKAHGAQVMIEGPGHIPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAML
CYVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGHPRAQERDDALSTARFEFRWNDQFALSLDPDTAREFHDETLPAE
PAKTAHFCSMCGPKFCSMRITQDVREYAAEHGLETEADIEAVLAAGMAEKSREFAEHGNRVYLPITQ

Sequences:

>Translated_547_residues
MTITVEPSVTTGPIAGSAKAYREIEAPGSGATLQVPFRRVHLSTGDHFDLYDTSGPYTDTDTVIDLTAGLPHRPGVVRDR
GTQLQRARAGEITAEMAFIAAREDMSAELVRDEVARGRAVIPANHHHPESEPMIIGKAFAVKVNANIGNSAVTSSIAEEV
DKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTELTWEIYRDTVIEQCEQGVDYMTVH
AGVLLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFEELCDIFARYDVTFSLGDGLRPGSIADANDAAQFAEL
RTLGELTKIAKAHGAQVMIEGPGHIPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAML
CYVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGHPRAQERDDALSTARFEFRWNDQFALSLDPDTAREFHDETLPAE
PAKTAHFCSMCGPKFCSMRITQDVREYAAEHGLETEADIEAVLAAGMAEKSREFAEHGNRVYLPITQ
>Mature_546_residues
TITVEPSVTTGPIAGSAKAYREIEAPGSGATLQVPFRRVHLSTGDHFDLYDTSGPYTDTDTVIDLTAGLPHRPGVVRDRG
TQLQRARAGEITAEMAFIAAREDMSAELVRDEVARGRAVIPANHHHPESEPMIIGKAFAVKVNANIGNSAVTSSIAEEVD
KMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTELTWEIYRDTVIEQCEQGVDYMTVHA
GVLLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFEELCDIFARYDVTFSLGDGLRPGSIADANDAAQFAELR
TLGELTKIAKAHGAQVMIEGPGHIPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLC
YVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGHPRAQERDDALSTARFEFRWNDQFALSLDPDTAREFHDETLPAEP
AKTAHFCSMCGPKFCSMRITQDVREYAAEHGLETEADIEAVLAAGMAEKSREFAEHGNRVYLPITQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=607, Percent_Identity=58.3196046128501, Blast_Score=687, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_MYCBO (P66912)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854094.1
- EnsemblBacteria:   EBMYCT00000014828
- GeneID:   1091437
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0431c
- GeneTree:   EBGT00050000016394
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   MBOV233413:MB0431C-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 59898; Mature: 59767

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: BINDING 146-146 BINDING 175-175 BINDING 204-204 BINDING 240-240 BINDING 340-340 BINDING 367-367

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITVEPSVTTGPIAGSAKAYREIEAPGSGATLQVPFRRVHLSTGDHFDLYDTSGPYTDT
CEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECEEEEEECCCCCEEEECCCCCCCCC
DTVIDLTAGLPHRPGVVRDRGTQLQRARAGEITAEMAFIAAREDMSAELVRDEVARGRAV
CCEEEEECCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEE
IPANHHHPESEPMIIGKAFAVKVNANIGNSAVTSSIAEEVDKMVWATRWGADTIMDLSTG
ECCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
KNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTELTWEIYRDTVIEQCEQGVDYMTVH
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEHH
AGVLLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFEELCDIFARYDVTFSLG
HHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEECC
DGLRPGSIADANDAAQFAELRTLGELTKIAKAHGAQVMIEGPGHIPMHKIVENVRLEEEL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
CEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYVTPKEHLGLPDRKDVKDG
HHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCC
VIAYKIAAHAADLAKGHPRAQERDDALSTARFEFRWNDQFALSLDPDTAREFHDETLPAE
CEEEHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEECCEEEEEECCHHHHHHHCCCCCCC
PAKTAHFCSMCGPKFCSMRITQDVREYAAEHGLETEADIEAVLAAGMAEKSREFAEHGNR
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VYLPITQ
EEEEECC
>Mature Secondary Structure 
TITVEPSVTTGPIAGSAKAYREIEAPGSGATLQVPFRRVHLSTGDHFDLYDTSGPYTDT
EEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECEEEEEECCCCCEEEECCCCCCCCC
DTVIDLTAGLPHRPGVVRDRGTQLQRARAGEITAEMAFIAAREDMSAELVRDEVARGRAV
CCEEEEECCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEE
IPANHHHPESEPMIIGKAFAVKVNANIGNSAVTSSIAEEVDKMVWATRWGADTIMDLSTG
ECCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
KNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTELTWEIYRDTVIEQCEQGVDYMTVH
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEHH
AGVLLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFEELCDIFARYDVTFSLG
HHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEECC
DGLRPGSIADANDAAQFAELRTLGELTKIAKAHGAQVMIEGPGHIPMHKIVENVRLEEEL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
CEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYVTPKEHLGLPDRKDVKDG
HHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCC
VIAYKIAAHAADLAKGHPRAQERDDALSTARFEFRWNDQFALSLDPDTAREFHDETLPAE
CEEEHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEECCEEEEEECCHHHHHHHCCCCCCC
PAKTAHFCSMCGPKFCSMRITQDVREYAAEHGLETEADIEAVLAAGMAEKSREFAEHGNR
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VYLPITQ
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972