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The map label for this gene is thiE

Identifier: 121636328

GI number: 121636328

Start: 531165

End: 531833

Strand: Reverse

Name: thiE

Synonym: BCG_0453c

Alternate gene names: 121636328

Gene position: 531833-531165 (Counterclockwise)

Preceding gene: 121636334

Following gene: 121636326

Centisome position: 12.16

GC content: 70.25

Gene sequence:

>669_bases
GTGCACGAATCCCGTCTGGCATCGGCAAGGCTGTATCTGTGCACCGACGCCCGTCGGGAACGCGGCGACTTGGCTCAGTT
CGCCGAGGCCGCCCTGGCCGGCGGAGTGGACATCATCCAGTTGCGCGACAAGGGCTCACCCGGTGAGCTGCGGTTCGGCC
CGCTACAGGCGCGTGACGAACTGGCCGCCTGCGAGATCCTGGCCGACGCAGCGCACCGGTACGGCGCCCTATTCGCGGTC
AACGACCGCGCCGATATTGCCCGCGCGGCCGGCGCCGACGTGCTGCATCTGGGTCAACGCGACCTGCCCGTGAACGTCGC
ACGACAGATCCTGGCACCGGATACCCTCATCGGCCGGTCCACCCACGACCCCGACCAGGTTGCTGCGGCCGCCGCCGGCG
ACGCCGACTATTTCTGTGTCGGACCATGTTGGCCGACACCGACCAAGCCTGGCCGTGCGGCGCCGGGTCTGGGGCTGGTA
CGGGTCGCCGCCGAACTCGGCGGCGACGACAAGCCGTGGTTCGCTATCGGCGGCATCAACGCGCAACGGCTGCCGGCCGT
GCTCGATGCCGGCGCTCGCCGGATCGTGGTGGTGCGGGCGATCACCTCGGCTGACGACCCACGTGCGGCGGCCGAGCAGC
TCCGGTCGGCGCTTACAGCAGCGAACTGA

Upstream 100 bases:

>100_bases
GATCAGGTGTGACGGTAAGGGCAGGCGCGCCCACTCTCAGACCCCGCTCCCGGGACTCCCGTGTCTACAGGGTTCCACGC
TAGCGCGCTAGCGTCACGAT

Downstream 100 bases:

>100_bases
TCCGCCTACCCAGCGGCGAGGGCGCCTGATCCGCGTCGCCCGGCGTACACCAGAGGAAAACCCCGGCCTCGATCTGAATG
GTGCGCGGCAGCCGCTGCCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MHESRLASARLYLCTDARRERGDLAQFAEAALAGGVDIIQLRDKGSPGELRFGPLQARDELAACEILADAAHRYGALFAV
NDRADIARAAGADVLHLGQRDLPVNVARQILAPDTLIGRSTHDPDQVAAAAAGDADYFCVGPCWPTPTKPGRAAPGLGLV
RVAAELGGDDKPWFAIGGINAQRLPAVLDAGARRIVVVRAITSADDPRAAAEQLRSALTAAN

Sequences:

>Translated_222_residues
MHESRLASARLYLCTDARRERGDLAQFAEAALAGGVDIIQLRDKGSPGELRFGPLQARDELAACEILADAAHRYGALFAV
NDRADIARAAGADVLHLGQRDLPVNVARQILAPDTLIGRSTHDPDQVAAAAAGDADYFCVGPCWPTPTKPGRAAPGLGLV
RVAAELGGDDKPWFAIGGINAQRLPAVLDAGARRIVVVRAITSADDPRAAAEQLRSALTAAN
>Mature_222_residues
MHESRLASARLYLCTDARRERGDLAQFAEAALAGGVDIIQLRDKGSPGELRFGPLQARDELAACEILADAAHRYGALFAV
NDRADIARAAGADVLHLGQRDLPVNVARQILAPDTLIGRSTHDPDQVAAAAAGDADYFCVGPCWPTPTKPGRAAPGLGLV
RVAAELGGDDKPWFAIGGINAQRLPAVLDAGARRIVVVRAITSADDPRAAAEQLRSALTAAN

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=36.9230769230769, Blast_Score=81, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=230, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_MYCBO (P66917)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854085.1
- ProteinModelPortal:   P66917
- SMR:   P66917
- EnsemblBacteria:   EBMYCT00000014950
- GeneID:   1091426
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0422c
- GeneTree:   EBGT00050000015844
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   PRK00043
- BioCyc:   MBOV233413:MB0422C-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23272; Mature: 23272

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHESRLASARLYLCTDARRERGDLAQFAEAALAGGVDIIQLRDKGSPGELRFGPLQARDE
CCCHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCHHH
LAACEILADAAHRYGALFAVNDRADIARAAGADVLHLGQRDLPVNVARQILAPDTLIGRS
HHHHHHHHHHHHHHCEEEEECCHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCHHCCCC
THDPDQVAAAAAGDADYFCVGPCWPTPTKPGRAAPGLGLVRVAAELGGDDKPWFAIGGIN
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
AQRLPAVLDAGARRIVVVRAITSADDPRAAAEQLRSALTAAN
HHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHESRLASARLYLCTDARRERGDLAQFAEAALAGGVDIIQLRDKGSPGELRFGPLQARDE
CCCHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCHHH
LAACEILADAAHRYGALFAVNDRADIARAAGADVLHLGQRDLPVNVARQILAPDTLIGRS
HHHHHHHHHHHHHHCEEEEECCHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCHHCCCC
THDPDQVAAAAAGDADYFCVGPCWPTPTKPGRAAPGLGLVRVAAELGGDDKPWFAIGGIN
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
AQRLPAVLDAGARRIVVVRAITSADDPRAAAEQLRSALTAAN
HHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972