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The map label for this gene is pyrE

Identifier: 121636295

GI number: 121636295

Start: 488654

End: 489193

Strand: Reverse

Name: pyrE

Synonym: BCG_0420c

Alternate gene names: 121636295

Gene position: 489193-488654 (Counterclockwise)

Preceding gene: 121636296

Following gene: 121636294

Centisome position: 11.18

GC content: 68.15

Gene sequence:

>540_bases
GTGGCCGGACCTGACCGCGCAGAGTTGGCTGAGTTGGTGCGCCGGCTGTCGGTGGTGCACGGCCGCGTCACGCTGTCGTC
TGGCCGGGAGGCCGACTACTACGTCGACCTGCGTCGCGCCACCTTGCACCATCGGGCGTCCGCGCTGATCGGCCGGCTAA
TGCGCGAACTCACTGCCGACTGGGACTATTCGGTTGTCGGCGGCCTGACACTCGGGGCCGATCCCGTGGCGACCGCCATC
ATGCATGCCCCGGGCCGTCCGATCGACGCGTTCGTGGTCCGCAAGTCGGCGAAAGCCCATGGCATGCAGCGACTTATCGA
AGGGTCGGAGGTTACCGGGCAGCGGGTGCTGGTGGTAGAGGACACCAGTACCACCGGTAACTCCGCGCTGACGGCGGTGC
ACGCCGTCCAAGACGTGGGTGGTGAGGTTGTCGGCGTGGCTACCGTGGTGGATCGCGCCACCGGGGCCGCAGAGGCTATC
GAAGCCGAGGGACTGCGCTACCGCAGCGTGCTGGGCCTGGCCGATCTGGGGCTGGATTAG

Upstream 100 bases:

>100_bases
CCACCAACTACCAACACTGAGACCGCGGATGTCACGCCCGAGGCCACCGCGGTGACGCCGCATGTGATCGGCCTCCCGCG
TGGCGGCGTGAGGAGAAGCC

Downstream 100 bases:

>100_bases
GCGTCGGCGGCATCGCTGTGCGCATTCTCGTTGCCTGGGCCACCTGCGGGGCCGTCGTCCTGTCCGGTCTGACGGGCTGC
TCGGGTTCGAGTCATTCCGG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MAGPDRAELAELVRRLSVVHGRVTLSSGREADYYVDLRRATLHHRASALIGRLMRELTADWDYSVVGGLTLGADPVATAI
MHAPGRPIDAFVVRKSAKAHGMQRLIEGSEVTGQRVLVVEDTSTTGNSALTAVHAVQDVGGEVVGVATVVDRATGAAEAI
EAEGLRYRSVLGLADLGLD

Sequences:

>Translated_179_residues
MAGPDRAELAELVRRLSVVHGRVTLSSGREADYYVDLRRATLHHRASALIGRLMRELTADWDYSVVGGLTLGADPVATAI
MHAPGRPIDAFVVRKSAKAHGMQRLIEGSEVTGQRVLVVEDTSTTGNSALTAVHAVQDVGGEVVGVATVVDRATGAAEAI
EAEGLRYRSVLGLADLGLD
>Mature_178_residues
AGPDRAELAELVRRLSVVHGRVTLSSGREADYYVDLRRATLHHRASALIGRLMRELTADWDYSVVGGLTLGADPVATAIM
HAPGRPIDAFVVRKSAKAHGMQRLIEGSEVTGQRVLVVEDTSTTGNSALTAVHAVQDVGGEVVGVATVVDRATGAAEAIE
AEGLRYRSVLGLADLGLD

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=177, Percent_Identity=30.5084745762712, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508631, Length=158, Percent_Identity=30.379746835443, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_MYCBO (P0A5U1)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854052.1
- ProteinModelPortal:   P0A5U1
- SMR:   P0A5U1
- EnsemblBacteria:   EBMYCT00000016894
- GeneID:   1091316
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0389c
- GeneTree:   EBGT00050000017679
- HOGENOM:   HBG404341
- OMA:   LSYPRIR
- ProtClustDB:   PRK00455
- BioCyc:   MBOV233413:MB0389C-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 18893; Mature: 18761

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 94-94 BINDING 95-95 BINDING 98-98 BINDING 100-100 BINDING 124-124 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGPDRAELAELVRRLSVVHGRVTLSSGREADYYVDLRRATLHHRASALIGRLMRELTAD
CCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCC
WDYSVVGGLTLGADPVATAIMHAPGRPIDAFVVRKSAKAHGMQRLIEGSEVTGQRVLVVE
CCCHHHCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
DTSTTGNSALTAVHAVQDVGGEVVGVATVVDRATGAAEAIEAEGLRYRSVLGLADLGLD
CCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGPDRAELAELVRRLSVVHGRVTLSSGREADYYVDLRRATLHHRASALIGRLMRELTAD
CCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCC
WDYSVVGGLTLGADPVATAIMHAPGRPIDAFVVRKSAKAHGMQRLIEGSEVTGQRVLVVE
CCCHHHCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
DTSTTGNSALTAVHAVQDVGGEVVGVATVVDRATGAAEAIEAEGLRYRSVLGLADLGLD
CCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972