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The map label for this gene is nikB [C]

Identifier: 121610360

GI number: 121610360

Start: 3800404

End: 3801402

Strand: Direct

Name: nikB [C]

Synonym: Veis_3428

Alternate gene names: 121610360

Gene position: 3800404-3801402 (Clockwise)

Preceding gene: 121610359

Following gene: 121610362

Centisome position: 68.27

GC content: 68.37

Gene sequence:

>999_bases
ATGTCCGCAGACAAGACCACGGCGGCCGACGGCAGCGCGTTGCGCCGCCTGTGCCGCCTGCGCCACCACCTGCTCGCGCG
CCGGCTGATGCAGGCTTTGCCGGTGCTGCTGTTTGCCACCTTCGTCGTCTTCGGCTTGCTCAAACTCGTGCCGGGCGACA
TCGCCGTGGCGCTGGCCGGGGACAACGCCAGCGACGCGCGCCTGGCCGAGATCCGGGCGCTCTACGGGCTGGACCAGCCC
TTTCTGGTGCAATACGGCCACTGGCTGTGGCAGGCGCTGCAGGGCGACCTGTCCCAGTCGCTGATGTCCGGCGAAGCGGT
GACCGTGTCGATCGCGCGCTGCCTGCCGAAAACCGCGCTGATCGTCGGCCTGGCACTGCTGATCGCGCTGGCGGTCGGCA
TCCCGCTGGGCATGTGGGCCGCCGCAAAGTGCGGCACCTGGGTCGATGGCTGGGTGATGACGCTGGCCTCGCTCGGTGTC
GCCGTGCCGAACTTCTGGCTCGCGATGGTGCTGATCGCCTGGTTCGCCTTGCAACTGAACTGGCTGCCGGCGACAGGCTC
GGCCTCGCTCGGCGCCGAGCCTGTCGAGGCGCTGCGCCATGCCTTGCTGCCGGCGCTGGCGCTGGCCGCCGGCGGCATCG
CCGAGGTGTCGCGGCAACTGCGCAGTTCGCTGGTCGACCTGCTGTCGTCGCAGCAGGTGCGCACGCTGCACGCCAAGGGC
CTGTCGCCTGCGGCCATCCTTTGCAAGCATGGCCTGAAGAATGTCGGCGTGAACTTGCTGACGGTGGTCACCCTGCTGGC
CAACCGGATGCTGGCCGCCACGGTGGTGATCGAGGCGGTGTTCGCGATCCCGGGCATGGGCGGGCTGATCGTGCAGGGCG
CGCTGTCGCGCGACTTCCCCGTGGTGCAGGGCGTGGTGTTCACGATGGTGCTGATCGTGATCACGATCAACCTGGGTGCC
GATTTGCTATATGGCGTGATCGATCCGAGGGTCAAGTAA

Upstream 100 bases:

>100_bases
CCTTTCAATTCGAGATCGACGCCTTGTCCAACGCGGTGCAGGGCTTCGTTCCGAATCTGCTGGGCAAGCCGAAATTCAAC
AACCTGTCCTTGTCCGGCTG

Downstream 100 bases:

>100_bases
AAAGTCTCGCGCATCACGACCATGGCAGGCAAAGCCAGAACCGTCGATGCGCCACGGGCGCGAGCACAAAGTCAGAGCAC
ACTGCGCGGAAAGCATCCAC

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSADKTTAADGSALRRLCRLRHHLLARRLMQALPVLLFATFVVFGLLKLVPGDIAVALAGDNASDARLAEIRALYGLDQP
FLVQYGHWLWQALQGDLSQSLMSGEAVTVSIARCLPKTALIVGLALLIALAVGIPLGMWAAAKCGTWVDGWVMTLASLGV
AVPNFWLAMVLIAWFALQLNWLPATGSASLGAEPVEALRHALLPALALAAGGIAEVSRQLRSSLVDLLSSQQVRTLHAKG
LSPAAILCKHGLKNVGVNLLTVVTLLANRMLAATVVIEAVFAIPGMGGLIVQGALSRDFPVVQGVVFTMVLIVITINLGA
DLLYGVIDPRVK

Sequences:

>Translated_332_residues
MSADKTTAADGSALRRLCRLRHHLLARRLMQALPVLLFATFVVFGLLKLVPGDIAVALAGDNASDARLAEIRALYGLDQP
FLVQYGHWLWQALQGDLSQSLMSGEAVTVSIARCLPKTALIVGLALLIALAVGIPLGMWAAAKCGTWVDGWVMTLASLGV
AVPNFWLAMVLIAWFALQLNWLPATGSASLGAEPVEALRHALLPALALAAGGIAEVSRQLRSSLVDLLSSQQVRTLHAKG
LSPAAILCKHGLKNVGVNLLTVVTLLANRMLAATVVIEAVFAIPGMGGLIVQGALSRDFPVVQGVVFTMVLIVITINLGA
DLLYGVIDPRVK
>Mature_331_residues
SADKTTAADGSALRRLCRLRHHLLARRLMQALPVLLFATFVVFGLLKLVPGDIAVALAGDNASDARLAEIRALYGLDQPF
LVQYGHWLWQALQGDLSQSLMSGEAVTVSIARCLPKTALIVGLALLIALAVGIPLGMWAAAKCGTWVDGWVMTLASLGVA
VPNFWLAMVLIAWFALQLNWLPATGSASLGAEPVEALRHALLPALALAAGGIAEVSRQLRSSLVDLLSSQQVRTLHAKGL
SPAAILCKHGLKNVGVNLLTVVTLLANRMLAATVVIEAVFAIPGMGGLIVQGALSRDFPVVQGVVFTMVLIVITINLGAD
LLYGVIDPRVK

Specific function: Probably part of an ABC transporter complex that could be involved in peptide import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=309, Percent_Identity=35.2750809061489, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787053, Length=307, Percent_Identity=35.5048859934853, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789965, Length=334, Percent_Identity=32.0359281437126, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787497, Length=308, Percent_Identity=32.4675324675325, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787761, Length=311, Percent_Identity=35.6913183279743, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788504, Length=278, Percent_Identity=32.3741007194245, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1787550, Length=265, Percent_Identity=27.9245283018868, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35063; Mature: 34932

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKTTAADGSALRRLCRLRHHLLARRLMQALPVLLFATFVVFGLLKLVPGDIAVALAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
DNASDARLAEIRALYGLDQPFLVQYGHWLWQALQGDLSQSLMSGEAVTVSIARCLPKTAL
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IVGLALLIALAVGIPLGMWAAAKCGTWVDGWVMTLASLGVAVPNFWLAMVLIAWFALQLN
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WLPATGSASLGAEPVEALRHALLPALALAAGGIAEVSRQLRSSLVDLLSSQQVRTLHAKG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCC
LSPAAILCKHGLKNVGVNLLTVVTLLANRMLAATVVIEAVFAIPGMGGLIVQGALSRDFP
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCH
VVQGVVFTMVLIVITINLGADLLYGVIDPRVK
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SADKTTAADGSALRRLCRLRHHLLARRLMQALPVLLFATFVVFGLLKLVPGDIAVALAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC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HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]