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The map label for this gene is gsiB [H]

Identifier: 121610359

GI number: 121610359

Start: 3798824

End: 3800404

Strand: Direct

Name: gsiB [H]

Synonym: Veis_3427

Alternate gene names: 121610359

Gene position: 3798824-3800404 (Clockwise)

Preceding gene: 121610358

Following gene: 121610360

Centisome position: 68.24

GC content: 64.77

Gene sequence:

>1581_bases
ATGAGTCAGCGTCGAGATGACAGGGTTTCACGGCTTCCTTCGAGCGCCTTGCAGCGCCGCGACGCTTTGGGCCTGGCGGG
CTTGGGTCTGGCGGGCCTGGGCCTGGGCTGGCCCGTGTTCGCGCAGACCGCGCCGAAGAAGGGTGGCGTGCTGAGGATCG
CGAGTCCGGCCAATCCTTCGAGTCTCGACCCGGCGGTGGGTGGCGCCGGCTCCGACCATGCCATCTTGTGGACGATGTAC
GACACGCTGGTCGAATGGGACTACGCCACACTCAAGCCCAAGCCCGGCATGGCCGAATGGATCTTTCCGGACCCGAAGAC
CATGCTGCTGCGCATCAAGCCGGGCATCTCGTTTCATGACGGCACGCCGTGCGACGCCGAGGCCGTGCGCTGGAACATCG
AGCGCAATCGCGGCGATGCGGCCTCGAACCTGAAGGCCGACCTGTCGAGCATCGAGGCCAGCGCCGTCGTCAGCCCGACG
CAGGTCAAGCTCATGCTCGACCAACCCGACGCGGCGCTCCCCGCCGTCTTTTCCGACCGTGCGGGCATGATGGTTTCGCC
CACGGCCCAGAAGGCGGCGGGCAAGGCTTTCGGCCGCAATCCGGTGGGCGCAGGGCCGTGGCGGTTTGTCAGTTGGGCCG
ACAACCAAAAGGTGATCGTCACGCGCCACGCGCAGTACTGGCGCACCGGCCTGCCGTACCTCGACGGCATCGAGTTTTCG
ATCATTCCCGAACTGGCCACCGGCCTGCGCTCGGTGGTGTCCCGGCAGAACGATCTGGCCTACCAGCTATCGGCGCGCTA
CCGCCCGATCATTACGCGCGAGAAGGCGCTCCGGCTGGTGACAGGCCCCACGCTCTACTGCATACAGCTGTACATGAATT
ACGCGCGGGCGCCGCTGGACAACCCGAAGCTGCGGCAGGCGATCAACTTCGCGCTGAACCGCGAGGCTTTCGTCAAGGCC
ACGATGGACGGTGTTGGCGAGCCCGCGCATATGAACCTGCCGAGCACCCACTGGGCCTACGACAAGAGCGTGGCCGGCCT
GTATCCGCATGACGTGGCAAAGGCCAGGCAACTGATGGCCGACGCCGGGCTGGCCGCCGGCATCGATCTGACGATCGGCG
GCTACACCGACCAGGACGCAGTGCGCCGCGGCGAAATCGTGATGGATCAACTCGGCAAGATCGGCATCCGCCTGAAGTTC
ACGAACGGCACGATCCCCGAGATCAGCGGCCAGTTCTTCGGCACAGAAAAGAAGTTCGACATGCTGCTGTCGGCCTGGAC
TGGCCGCCCCGACCCGAGCATGAGCTACTCGCTGATGTACGCCAAGGGCGCCTACTACAACGCCGGGCGCAGCGAGTTCT
CGCCGGAACTCTCGGCACTGCTGCAGGAAAGCCGCGCCAGGGAAGATATCCAGTGGCGCAAAAGCGTGTTCGCCAAAATC
CAGCGCATGGTGATGGAAAACGCGCTGGTGGCGCCGCTGGCCTTTCAATTCGAGATCGACGCCTTGTCCAACGCGGTGCA
GGGCTTCGTTCCGAATCTGCTGGGCAAGCCGAAATTCAACAACCTGTCCTTGTCCGGCTGA

Upstream 100 bases:

>100_bases
CGGCGGCAGGGCCGCCCGCAGGCAACGGCGGCGCGGCCGCCGGTTGCCATCGCCCCGGCACCACCGACCACGGCATCGTT
CACACCGCATGGGAGACAGC

Downstream 100 bases:

>100_bases
TGTCCGCAGACAAGACCACGGCGGCCGACGGCAGCGCGTTGCGCCGCCTGTGCCGCCTGCGCCACCACCTGCTCGCGCGC
CGGCTGATGCAGGCTTTGCC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSQRRDDRVSRLPSSALQRRDALGLAGLGLAGLGLGWPVFAQTAPKKGGVLRIASPANPSSLDPAVGGAGSDHAILWTMY
DTLVEWDYATLKPKPGMAEWIFPDPKTMLLRIKPGISFHDGTPCDAEAVRWNIERNRGDAASNLKADLSSIEASAVVSPT
QVKLMLDQPDAALPAVFSDRAGMMVSPTAQKAAGKAFGRNPVGAGPWRFVSWADNQKVIVTRHAQYWRTGLPYLDGIEFS
IIPELATGLRSVVSRQNDLAYQLSARYRPIITREKALRLVTGPTLYCIQLYMNYARAPLDNPKLRQAINFALNREAFVKA
TMDGVGEPAHMNLPSTHWAYDKSVAGLYPHDVAKARQLMADAGLAAGIDLTIGGYTDQDAVRRGEIVMDQLGKIGIRLKF
TNGTIPEISGQFFGTEKKFDMLLSAWTGRPDPSMSYSLMYAKGAYYNAGRSEFSPELSALLQESRAREDIQWRKSVFAKI
QRMVMENALVAPLAFQFEIDALSNAVQGFVPNLLGKPKFNNLSLSG

Sequences:

>Translated_526_residues
MSQRRDDRVSRLPSSALQRRDALGLAGLGLAGLGLGWPVFAQTAPKKGGVLRIASPANPSSLDPAVGGAGSDHAILWTMY
DTLVEWDYATLKPKPGMAEWIFPDPKTMLLRIKPGISFHDGTPCDAEAVRWNIERNRGDAASNLKADLSSIEASAVVSPT
QVKLMLDQPDAALPAVFSDRAGMMVSPTAQKAAGKAFGRNPVGAGPWRFVSWADNQKVIVTRHAQYWRTGLPYLDGIEFS
IIPELATGLRSVVSRQNDLAYQLSARYRPIITREKALRLVTGPTLYCIQLYMNYARAPLDNPKLRQAINFALNREAFVKA
TMDGVGEPAHMNLPSTHWAYDKSVAGLYPHDVAKARQLMADAGLAAGIDLTIGGYTDQDAVRRGEIVMDQLGKIGIRLKF
TNGTIPEISGQFFGTEKKFDMLLSAWTGRPDPSMSYSLMYAKGAYYNAGRSEFSPELSALLQESRAREDIQWRKSVFAKI
QRMVMENALVAPLAFQFEIDALSNAVQGFVPNLLGKPKFNNLSLSG
>Mature_525_residues
SQRRDDRVSRLPSSALQRRDALGLAGLGLAGLGLGWPVFAQTAPKKGGVLRIASPANPSSLDPAVGGAGSDHAILWTMYD
TLVEWDYATLKPKPGMAEWIFPDPKTMLLRIKPGISFHDGTPCDAEAVRWNIERNRGDAASNLKADLSSIEASAVVSPTQ
VKLMLDQPDAALPAVFSDRAGMMVSPTAQKAAGKAFGRNPVGAGPWRFVSWADNQKVIVTRHAQYWRTGLPYLDGIEFSI
IPELATGLRSVVSRQNDLAYQLSARYRPIITREKALRLVTGPTLYCIQLYMNYARAPLDNPKLRQAINFALNREAFVKAT
MDGVGEPAHMNLPSTHWAYDKSVAGLYPHDVAKARQLMADAGLAAGIDLTIGGYTDQDAVRRGEIVMDQLGKIGIRLKFT
NGTIPEISGQFFGTEKKFDMLLSAWTGRPDPSMSYSLMYAKGAYYNAGRSEFSPELSALLQESRAREDIQWRKSVFAKIQ
RMVMENALVAPLAFQFEIDALSNAVQGFVPNLLGKPKFNNLSLSG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=500, Percent_Identity=27, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1789966, Length=544, Percent_Identity=24.6323529411765, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787762, Length=512, Percent_Identity=25.9765625, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787551, Length=533, Percent_Identity=22.5140712945591, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789887, Length=433, Percent_Identity=23.5565819861432, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57578; Mature: 57446

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRRDDRVSRLPSSALQRRDALGLAGLGLAGLGLGWPVFAQTAPKKGGVLRIASPANPS
CCCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHCCCCCCCCEEEEECCCCCC
SLDPAVGGAGSDHAILWTMYDTLVEWDYATLKPKPGMAEWIFPDPKTMLLRIKPGISFHD
CCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCC
GTPCDAEAVRWNIERNRGDAASNLKADLSSIEASAVVSPTQVKLMLDQPDAALPAVFSDR
CCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHCCC
AGMMVSPTAQKAAGKAFGRNPVGAGPWRFVSWADNQKVIVTRHAQYWRTGLPYLDGIEFS
CCEEECCCHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECHHHHHCCCCCCCCCCEE
IIPELATGLRSVVSRQNDLAYQLSARYRPIITREKALRLVTGPTLYCIQLYMNYARAPLD
EHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEHHHHHEEECCCHHHHHHHHHHHHHCCCC
NPKLRQAINFALNREAFVKATMDGVGEPAHMNLPSTHWAYDKSVAGLYPHDVAKARQLMA
CHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
DAGLAAGIDLTIGGYTDQDAVRRGEIVMDQLGKIGIRLKFTNGTIPEISGQFFGTEKKFD
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHH
MLLSAWTGRPDPSMSYSLMYAKGAYYNAGRSEFSPELSALLQESRAREDIQWRKSVFAKI
HHHHHHCCCCCCCCEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QRMVMENALVAPLAFQFEIDALSNAVQGFVPNLLGKPKFNNLSLSG
HHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQRRDDRVSRLPSSALQRRDALGLAGLGLAGLGLGWPVFAQTAPKKGGVLRIASPANPS
CCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHCCCCCCCCEEEEECCCCCC
SLDPAVGGAGSDHAILWTMYDTLVEWDYATLKPKPGMAEWIFPDPKTMLLRIKPGISFHD
CCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCC
GTPCDAEAVRWNIERNRGDAASNLKADLSSIEASAVVSPTQVKLMLDQPDAALPAVFSDR
CCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHCCC
AGMMVSPTAQKAAGKAFGRNPVGAGPWRFVSWADNQKVIVTRHAQYWRTGLPYLDGIEFS
CCEEECCCHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECHHHHHCCCCCCCCCCEE
IIPELATGLRSVVSRQNDLAYQLSARYRPIITREKALRLVTGPTLYCIQLYMNYARAPLD
EHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEHHHHHEEECCCHHHHHHHHHHHHHCCCC
NPKLRQAINFALNREAFVKATMDGVGEPAHMNLPSTHWAYDKSVAGLYPHDVAKARQLMA
CHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
DAGLAAGIDLTIGGYTDQDAVRRGEIVMDQLGKIGIRLKFTNGTIPEISGQFFGTEKKFD
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHH
MLLSAWTGRPDPSMSYSLMYAKGAYYNAGRSEFSPELSALLQESRAREDIQWRKSVFAKI
HHHHHHCCCCCCCCEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QRMVMENALVAPLAFQFEIDALSNAVQGFVPNLLGKPKFNNLSLSG
HHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA