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The map label for this gene is kstD [H]

Identifier: 121610302

GI number: 121610302

Start: 3739427

End: 3739846

Strand: Direct

Name: kstD [H]

Synonym: Veis_3367

Alternate gene names: 121610302

Gene position: 3739427-3739846 (Clockwise)

Preceding gene: 121610300

Following gene: 121610309

Centisome position: 67.17

GC content: 66.19

Gene sequence:

>420_bases
GTGGACCCGAGCGCGCTGCAGCGGACCATCGCCGAATTCAACGAGCAGGCGCGCCCGGGGATCGACCTTCTATTCGGCAA
GGGCGCCAGCGCGTTTGATCGCTTCTACGGGGATCCGGCACATGCCGGCCCGAACAACTGCATGGGGCCGCTGGAGACGC
CGCCGTTTTACGTGATCCGGATCACTGCCGGCAGCATGGGCACACTGGCAGGCTTGAAGACCGATGCCGTCGGCCGCGCA
TTGAACGGCAATGGACGCGCCATCGACGGCTTGTATGCCGTCGGTAACGACATGGCCAATGTATTCCAGGGTGCCTGCCC
CGGCGGGGGCATCACCCTCGGGCCCGGAATGACCTTCGGCTACCTGGCCGGCCTGCACGCAGCCGATCGTCTGTCGGCAC
ACCCGGTGGCGGCTGTGTAA

Upstream 100 bases:

>100_bases
GCGACTTCATCGACCCCTGGCCGGCGTCCCTGGCGCCGCATCTGCGCTCGGCTACCTGAAGACGGGCCAGGACATCGCTG
AACTGGCGGCCGCCATCGGA

Downstream 100 bases:

>100_bases
GCCATCACCGCAACGACCGTGATGGAGAAGTGAACATGTCCGAACTGCGCAACTACTGGTACATCGCATGCCCCCTCAAG
CCAGCTCGGCGATCGGCCAC

Product: hypothetical protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MDPSALQRTIAEFNEQARPGIDLLFGKGASAFDRFYGDPAHAGPNNCMGPLETPPFYVIRITAGSMGTLAGLKTDAVGRA
LNGNGRAIDGLYAVGNDMANVFQGACPGGGITLGPGMTFGYLAGLHAADRLSAHPVAAV

Sequences:

>Translated_139_residues
MDPSALQRTIAEFNEQARPGIDLLFGKGASAFDRFYGDPAHAGPNNCMGPLETPPFYVIRITAGSMGTLAGLKTDAVGRA
LNGNGRAIDGLYAVGNDMANVFQGACPGGGITLGPGMTFGYLAGLHAADRLSAHPVAAV
>Mature_139_residues
MDPSALQRTIAEFNEQARPGIDLLFGKGASAFDRFYGDPAHAGPNNCMGPLETPPFYVIRITAGSMGTLAGLKTDAVGRA
LNGNGRAIDGLYAVGNDMANVFQGACPGGGITLGPGMTFGYLAGLHAADRLSAHPVAAV

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 14222; Mature: 14222

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSALQRTIAEFNEQARPGIDLLFGKGASAFDRFYGDPAHAGPNNCMGPLETPPFYVIR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
ITAGSMGTLAGLKTDAVGRALNGNGRAIDGLYAVGNDMANVFQGACPGGGITLGPGMTFG
ECCCCCCHHHCCCHHHHCCEECCCCCEECHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
YLAGLHAADRLSAHPVAAV
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDPSALQRTIAEFNEQARPGIDLLFGKGASAFDRFYGDPAHAGPNNCMGPLETPPFYVIR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
ITAGSMGTLAGLKTDAVGRALNGNGRAIDGLYAVGNDMANVFQGACPGGGITLGPGMTFG
ECCCCCCHHHCCCHHHHCCEECCCCCEECHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
YLAGLHAADRLSAHPVAAV
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]