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The map label for this gene is gph [C]

Identifier: 121610300

GI number: 121610300

Start: 3737235

End: 3737900

Strand: Direct

Name: gph [C]

Synonym: Veis_3364

Alternate gene names: 121610300

Gene position: 3737235-3737900 (Clockwise)

Preceding gene: 121610299

Following gene: 121610302

Centisome position: 67.13

GC content: 64.11

Gene sequence:

>666_bases
ATGACGGAGGCGGTTCTGTTCGATCTCGACGGCACGCTCGCTGACACGAGCGGCGATTTGGCTGCCGCGACCTGCGATGC
GCTCGCTGCAGTGGGTTTGCCCTCGCCTGAGCCACACACCCTGCATGCCTATGGTTCGCGCGGCGGCCGCGGCATGCTGC
GCGCGGGCTGGAGCGGGGATTTGGATGACGCTTTGTTCCAGCGCGCCTTCACGGTTTTTCTGGAGCGCTATGCGCTGCGG
ATGTATGAGTCGACTGTGCTGTTCGACGGCGTTGGCGCTTGCCTGGAGCAACTCGCGCGAGGCGGGCGGCGCTGGGGCAT
CGTCACCAACAAAAGGGAGCGCTACACGCGGCCGCTGGTGAAGCATCTCGGGTTTCAGCCCGAAGTCCTGGTGTGTGGCG
ACATGGTGCCGCAGTCCAAGCCCGCGCCTGACAGTCTGCTTGCAGCAGCCGCTGCGCTGGGGCATGCGCCTGCTGAATGC
GTGTATGTCGGGGACGATGGAAAGGATGTCATTGCGGCCGCGGCTGCGGGCATGACCTCGGTGGCTGCGTTGTATGGGTA
TTCGGTGCGACTGAATCACCCGGGTCTCAAAGGCGCAGACGCAGAGATCGGGACATTGCGCGCGCTGCCTAGCGTCCTGA
AGGACCTCGCACAACGGAAATCATAA

Upstream 100 bases:

>100_bases
GCCTCGACGCGCTGGGCAGCAGCTCGGAACAGGCCGCGCGCTTGCAGGTGCAACTGCGCGCAATTGCGCAGGTGCCGCAA
AGCTGGCTGCTGGCGGTGAC

Downstream 100 bases:

>100_bases
AATGGCGAAGAATGTGAGATTCAACTCACAGACGCCTTTCGATGTCGCCGGTTTTGGTACCTCGGTGAAGGACTTGGGGT
GAAAGCTGATGCGCTACATC

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTEAVLFDLDGTLADTSGDLAAATCDALAAVGLPSPEPHTLHAYGSRGGRGMLRAGWSGDLDDALFQRAFTVFLERYALR
MYESTVLFDGVGACLEQLARGGRRWGIVTNKRERYTRPLVKHLGFQPEVLVCGDMVPQSKPAPDSLLAAAAALGHAPAEC
VYVGDDGKDVIAAAAAGMTSVAALYGYSVRLNHPGLKGADAEIGTLRALPSVLKDLAQRKS

Sequences:

>Translated_221_residues
MTEAVLFDLDGTLADTSGDLAAATCDALAAVGLPSPEPHTLHAYGSRGGRGMLRAGWSGDLDDALFQRAFTVFLERYALR
MYESTVLFDGVGACLEQLARGGRRWGIVTNKRERYTRPLVKHLGFQPEVLVCGDMVPQSKPAPDSLLAAAAALGHAPAEC
VYVGDDGKDVIAAAAAGMTSVAALYGYSVRLNHPGLKGADAEIGTLRALPSVLKDLAQRKS
>Mature_220_residues
TEAVLFDLDGTLADTSGDLAAATCDALAAVGLPSPEPHTLHAYGSRGGRGMLRAGWSGDLDDALFQRAFTVFLERYALRM
YESTVLFDGVGACLEQLARGGRRWGIVTNKRERYTRPLVKHLGFQPEVLVCGDMVPQSKPAPDSLLAAAAALGHAPAECV
YVGDDGKDVIAAAAAGMTSVAALYGYSVRLNHPGLKGADAEIGTLRALPSVLKDLAQRKS

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=206, Percent_Identity=35.4368932038835, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23301; Mature: 23170

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAVLFDLDGTLADTSGDLAAATCDALAAVGLPSPEPHTLHAYGSRGGRGMLRAGWSGD
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEECCCCCC
LDDALFQRAFTVFLERYALRMYESTVLFDGVGACLEQLARGGRRWGIVTNKRERYTRPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEECCCHHHHHHHHH
KHLGFQPEVLVCGDMVPQSKPAPDSLLAAAAALGHAPAECVYVGDDGKDVIAAAAAGMTS
HHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHH
VAALYGYSVRLNHPGLKGADAEIGTLRALPSVLKDLAQRKS
HHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEAVLFDLDGTLADTSGDLAAATCDALAAVGLPSPEPHTLHAYGSRGGRGMLRAGWSGD
CCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEECCCCCC
LDDALFQRAFTVFLERYALRMYESTVLFDGVGACLEQLARGGRRWGIVTNKRERYTRPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEECCCHHHHHHHHH
KHLGFQPEVLVCGDMVPQSKPAPDSLLAAAAALGHAPAECVYVGDDGKDVIAAAAAGMTS
HHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHH
VAALYGYSVRLNHPGLKGADAEIGTLRALPSVLKDLAQRKS
HHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA