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The map label for this gene is gcvA [H]

Identifier: 121610258

GI number: 121610258

Start: 3701055

End: 3701957

Strand: Direct

Name: gcvA [H]

Synonym: Veis_3317

Alternate gene names: 121610258

Gene position: 3701055-3701957 (Clockwise)

Preceding gene: 121610257

Following gene: 121610260

Centisome position: 66.49

GC content: 66.45

Gene sequence:

>903_bases
ATGATCCGTCGCATCCCCCCGTTGAACCCGCTGCGTGTCTTCGAGGTGGTGGCCCGAACGCACAATCTCACGGCCGCAGC
CGCCGAGTTGAACGTCACACAGTCGGCGGTGAGCCGGCAGATCGCAACGCTGGAAACCTACCTGGGGATGGAGCTGCTGC
GGCGCGAACGCCATGGCGTGAGTCTGACGCGCGCCGGGCACTCGTATGCGCAGCAACTGCTGCCGGCGTTCGAGCAGATC
GCCAGCGCCACCCGGGCGCTGGTCAAAGGCTCCGGCGAGCGCGCACTGCGGGTGCGCACCTACACCACCTTTGCTGCCAA
GTGGCTGATTCCCCGGCTCGGCGAGTTTCGCCGCCTGCATCCCGGCATCGAGGTCAATATCACCAACGCCGTGCCCGATG
TCGACTTCGACCGCGACAAGGCGGATGTCGCCGTGCAGTTCGGCGCCGGCCGCTGGCCCCGGACGCAGGCGCTGTTTCTG
TTTGGCGACGAAATCGAGCCGGTGTGCTCGCCCGCTTACCTGAAGCAATACGCGCCCAACGCCAAATTCCCGGTCTCGCT
GCTGCGCCAGCAATTGCTGGTCTCCCACTACCGGCGCAGCGACTGGTCGGTGTGGCTGGCAGCGACGGGCCTGGCGCATG
AGGCCAGGGATGCGCTGCAGATGACCTTCGGCAGTTCGGTGCTGACCTGGCAGGCCGCGATCGACGGGCTGGGCATGGCC
ATCGGACAAAACCGCCTGCTGGCCGTCGAGTTCGACAAGGGTCTGCTGGTGCGGCCCTTTGCGCAATTTGCCAAGCCGGT
GCAACGCGATCAGGGCTACTACCTGGTGTGGCCGCTGCGCTCGCGCGAGTCGCGCAAGCGCGCGCTGTTTCGTGATTGGT
TGCTGAGCGCCTGCGCGCAGTAA

Upstream 100 bases:

>100_bases
TGCCCGCTGCGGCCAGCGCAGACCCCGGCGCTCCCGGCCTGCGCACCCAGGCCCGACCCGGGCCGCCTTGCGGTGAGGCG
GGGTCGCTGAGGGCAACGGG

Downstream 100 bases:

>100_bases
GTGTGCGCAGGCGGTCTGGGGCGCCCGCGCCGGGCGACGGATGCCGTGGACATGACATGCGTGGCCCGCGCCCGGTCAGG
GCAGCCGCAGGCTGGGCATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIRRIPPLNPLRVFEVVARTHNLTAAAAELNVTQSAVSRQIATLETYLGMELLRRERHGVSLTRAGHSYAQQLLPAFEQI
ASATRALVKGSGERALRVRTYTTFAAKWLIPRLGEFRRLHPGIEVNITNAVPDVDFDRDKADVAVQFGAGRWPRTQALFL
FGDEIEPVCSPAYLKQYAPNAKFPVSLLRQQLLVSHYRRSDWSVWLAATGLAHEARDALQMTFGSSVLTWQAAIDGLGMA
IGQNRLLAVEFDKGLLVRPFAQFAKPVQRDQGYYLVWPLRSRESRKRALFRDWLLSACAQ

Sequences:

>Translated_300_residues
MIRRIPPLNPLRVFEVVARTHNLTAAAAELNVTQSAVSRQIATLETYLGMELLRRERHGVSLTRAGHSYAQQLLPAFEQI
ASATRALVKGSGERALRVRTYTTFAAKWLIPRLGEFRRLHPGIEVNITNAVPDVDFDRDKADVAVQFGAGRWPRTQALFL
FGDEIEPVCSPAYLKQYAPNAKFPVSLLRQQLLVSHYRRSDWSVWLAATGLAHEARDALQMTFGSSVLTWQAAIDGLGMA
IGQNRLLAVEFDKGLLVRPFAQFAKPVQRDQGYYLVWPLRSRESRKRALFRDWLLSACAQ
>Mature_300_residues
MIRRIPPLNPLRVFEVVARTHNLTAAAAELNVTQSAVSRQIATLETYLGMELLRRERHGVSLTRAGHSYAQQLLPAFEQI
ASATRALVKGSGERALRVRTYTTFAAKWLIPRLGEFRRLHPGIEVNITNAVPDVDFDRDKADVAVQFGAGRWPRTQALFL
FGDEIEPVCSPAYLKQYAPNAKFPVSLLRQQLLVSHYRRSDWSVWLAATGLAHEARDALQMTFGSSVLTWQAAIDGLGMA
IGQNRLLAVEFDKGLLVRPFAQFAKPVQRDQGYYLVWPLRSRESRKRALFRDWLLSACAQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=38.7959866220736, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=30.6896551724138, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=29.4915254237288, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786401, Length=262, Percent_Identity=24.8091603053435, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=26.7558528428094, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=275, Percent_Identity=25.0909090909091, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787128, Length=195, Percent_Identity=27.6923076923077, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789639, Length=176, Percent_Identity=27.8409090909091, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33811; Mature: 33811

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRIPPLNPLRVFEVVARTHNLTAAAAELNVTQSAVSRQIATLETYLGMELLRRERHGV
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLTRAGHSYAQQLLPAFEQIASATRALVKGSGERALRVRTYTTFAAKWLIPRLGEFRRLH
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
PGIEVNITNAVPDVDFDRDKADVAVQFGAGRWPRTQALFLFGDEIEPVCSPAYLKQYAPN
CCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCC
AKFPVSLLRQQLLVSHYRRSDWSVWLAATGLAHEARDALQMTFGSSVLTWQAAIDGLGMA
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
IGQNRLLAVEFDKGLLVRPFAQFAKPVQRDQGYYLVWPLRSRESRKRALFRDWLLSACAQ
CCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRRIPPLNPLRVFEVVARTHNLTAAAAELNVTQSAVSRQIATLETYLGMELLRRERHGV
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLTRAGHSYAQQLLPAFEQIASATRALVKGSGERALRVRTYTTFAAKWLIPRLGEFRRLH
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
PGIEVNITNAVPDVDFDRDKADVAVQFGAGRWPRTQALFLFGDEIEPVCSPAYLKQYAPN
CCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCC
AKFPVSLLRQQLLVSHYRRSDWSVWLAATGLAHEARDALQMTFGSSVLTWQAAIDGLGMA
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
IGQNRLLAVEFDKGLLVRPFAQFAKPVQRDQGYYLVWPLRSRESRKRALFRDWLLSACAQ
CCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]