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The map label for this gene is rimO

Identifier: 121610173

GI number: 121610173

Start: 3607813

End: 3609201

Strand: Direct

Name: rimO

Synonym: Veis_3232

Alternate gene names: 121610173

Gene position: 3607813-3609201 (Clockwise)

Preceding gene: 121610172

Following gene: 121610196

Centisome position: 64.81

GC content: 67.67

Gene sequence:

>1389_bases
ATGACCACAATGACCGCAGAGCGCGCCCCGCGCATAGGCATGGTGTCGCTCGGCTGCCCCAAGGCCCTGACCGACTCCGA
ACTCATCCTGACGCAGTTGAGCGCCGAGGGCTACGAGACCTCCAAGACCTTTCAGGGCGCGGATTTGGTCATCGTCAACA
CCTGCGGCTTCATCGACGATGCGGTCAAGGAAAGCCTGGACACCATTGGCGAGGCGCTGGCCGAGAACGGCAAGGTCATC
GTCACCGGCTGCCTGGGCGCGCGCACCGGCGCCGACGGCGGCAACATGGTGCGCCAGTTGCACCCCAGCGTACTGGCCGT
GACCGGGCCCCAGGCCACGCAGCAGGTGCTGGACGCAGTCCACCGGAACCTGCCCAAGCCGCACGACCCCTTCATCGATC
TGGTGCCCGGGGGCATGGGCATTGCGGGCCTGAAGCTCACGCCCAGGCATTACGCCTACCTGAAGATCAGCGAGGGCTGC
AACCACCGCTGCACGTTCTGCATCATCCCGACCCTGCGCGGCGCGCTGGTCTCGCGCCCGATTGGCGCTGTGCTGAACGA
GGCCAGGGCCTTGTTTGCCGGCGGCGTGAAAGAACTGCTGGTGATCAGCCAGGACAGCTCGGCCTATGGCGTGGACATGC
AGTACCGCACCGGTTTCTGGGACGGCCAACCGCTCAAGACCCGCCTGCTGGAACTGGTGCAGGCGCTGGGCGCGCTCGCC
GAGCCCTACGGCGCCTGGGTGCGGCTGCACTATGTGTACCCCTACCCGAGCGTCGATGCGCTCATCCCGCTGATGGCCCA
GGGCCGGGTGCTGCCCTATCTGGATGTGCCCTTGCAGCACAGCCACCCCGAGGTGCTGCGGCGCATGAAGCGCCCCGCCA
GCGGCGAGCGCAACCTGGAGCGCATACAGCGCTGGCGCGAAGTCTGCCCCGAGATCGTGATCCGCAGCAGCTTCATCGTC
GGCTTTCCCGGCGAGACGCAGGCCGAGTTCGAGCATCTACTGGACTTCCTGCGCGCAGCCCGAATCGACCGCGTCGGCTG
CTTTGCCTACAGCGACGTGAGCGGCGCCGTGGCCAACGATCTGCCCGGCATGTTGCCGATGCCACTGCGGCAAGAGCGCC
GCGCCCGTTTCATGGCCGTGGCCGAAGCGCTGTCGAGCGCCAAACTGCAACGCCGCGTGGGCGCCACGATGCAGGTGCTG
ATCGACGCTGCCCCGGGCCTGGGCCGCAAGGGCGGCGTAGGCCGCAGCTATGCCGACGCGCCGGAGATCGACGGCGCAGT
GCACCTGCTGCCGCCCGAAAAGATCAGCAAGACCCTGAAGGTGGGCGAGTTCACCCAGGCCCGCATCGTCGGCGTGCGCG
GGCATGACCTGCTGGCGCAGCCGATTTGA

Upstream 100 bases:

>100_bases
TGCTGGGAGCGTTTGGCCTCAAGCGCTGAAACGGGCGCCCGGCGCAAGCAGCCATGCGCCGCGCGCTGGGCCATATACGA
GACCTGAGATTGACATCCCC

Downstream 100 bases:

>100_bases
TCATCACACGCGCCTGCGGTATTCGCCGGTGCGGGTGTCGATTTCTATCCTGTCGCCTTGCGCCACGAACAGCGGCACCG
GCACCTCGAAGCCGGTGGCG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTTMTAERAPRIGMVSLGCPKALTDSELILTQLSAEGYETSKTFQGADLVIVNTCGFIDDAVKESLDTIGEALAENGKVI
VTGCLGARTGADGGNMVRQLHPSVLAVTGPQATQQVLDAVHRNLPKPHDPFIDLVPGGMGIAGLKLTPRHYAYLKISEGC
NHRCTFCIIPTLRGALVSRPIGAVLNEARALFAGGVKELLVISQDSSAYGVDMQYRTGFWDGQPLKTRLLELVQALGALA
EPYGAWVRLHYVYPYPSVDALIPLMAQGRVLPYLDVPLQHSHPEVLRRMKRPASGERNLERIQRWREVCPEIVIRSSFIV
GFPGETQAEFEHLLDFLRAARIDRVGCFAYSDVSGAVANDLPGMLPMPLRQERRARFMAVAEALSSAKLQRRVGATMQVL
IDAAPGLGRKGGVGRSYADAPEIDGAVHLLPPEKISKTLKVGEFTQARIVGVRGHDLLAQPI

Sequences:

>Translated_462_residues
MTTMTAERAPRIGMVSLGCPKALTDSELILTQLSAEGYETSKTFQGADLVIVNTCGFIDDAVKESLDTIGEALAENGKVI
VTGCLGARTGADGGNMVRQLHPSVLAVTGPQATQQVLDAVHRNLPKPHDPFIDLVPGGMGIAGLKLTPRHYAYLKISEGC
NHRCTFCIIPTLRGALVSRPIGAVLNEARALFAGGVKELLVISQDSSAYGVDMQYRTGFWDGQPLKTRLLELVQALGALA
EPYGAWVRLHYVYPYPSVDALIPLMAQGRVLPYLDVPLQHSHPEVLRRMKRPASGERNLERIQRWREVCPEIVIRSSFIV
GFPGETQAEFEHLLDFLRAARIDRVGCFAYSDVSGAVANDLPGMLPMPLRQERRARFMAVAEALSSAKLQRRVGATMQVL
IDAAPGLGRKGGVGRSYADAPEIDGAVHLLPPEKISKTLKVGEFTQARIVGVRGHDLLAQPI
>Mature_461_residues
TTMTAERAPRIGMVSLGCPKALTDSELILTQLSAEGYETSKTFQGADLVIVNTCGFIDDAVKESLDTIGEALAENGKVIV
TGCLGARTGADGGNMVRQLHPSVLAVTGPQATQQVLDAVHRNLPKPHDPFIDLVPGGMGIAGLKLTPRHYAYLKISEGCN
HRCTFCIIPTLRGALVSRPIGAVLNEARALFAGGVKELLVISQDSSAYGVDMQYRTGFWDGQPLKTRLLELVQALGALAE
PYGAWVRLHYVYPYPSVDALIPLMAQGRVLPYLDVPLQHSHPEVLRRMKRPASGERNLERIQRWREVCPEIVIRSSFIVG
FPGETQAEFEHLLDFLRAARIDRVGCFAYSDVSGAVANDLPGMLPMPLRQERRARFMAVAEALSSAKLQRRVGATMQVLI
DAAPGLGRKGGVGRSYADAPEIDGAVHLLPPEKISKTLKVGEFTQARIVGVRGHDLLAQPI

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=402, Percent_Identity=25.3731343283582, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI28872782, Length=443, Percent_Identity=22.7990970654628, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI28872784, Length=463, Percent_Identity=22.4622030237581, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1787057, Length=458, Percent_Identity=56.7685589519651, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1786882, Length=413, Percent_Identity=26.3922518159806, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17553146, Length=492, Percent_Identity=24.1869918699187, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71996771, Length=316, Percent_Identity=29.4303797468354, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356207, Length=490, Percent_Identity=23.469387755102, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922432, Length=406, Percent_Identity=26.8472906403941, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_VEREI (A1WMV5)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_997980.1
- ProteinModelPortal:   A1WMV5
- SMR:   A1WMV5
- STRING:   A1WMV5
- GeneID:   4694846
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_3232
- NMPDR:   fig|391735.5.peg.2995
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   A1WMV5
- ProtClustDB:   PRK14862
- BioCyc:   VEIS391735:VEIS_3232-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50125; Mature: 49994

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMTAERAPRIGMVSLGCPKALTDSELILTQLSAEGYETSKTFQGADLVIVNTCGFIDD
CCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHH
AVKESLDTIGEALAENGKVIVTGCLGARTGADGGNMVRQLHPSVLAVTGPQATQQVLDAV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHH
HRNLPKPHDPFIDLVPGGMGIAGLKLTPRHYAYLKISEGCNHRCTFCIIPTLRGALVSRP
HHCCCCCCCCCEEECCCCCCEECEEECCCEEEEEEECCCCCCCEEEEEECHHHHHHHHCH
IGAVLNEARALFAGGVKELLVISQDSSAYGVDMQYRTGFWDGQPLKTRLLELVQALGALA
HHHHHHHHHHHHHCCHHHEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
EPYGAWVRLHYVYPYPSVDALIPLMAQGRVLPYLDVPLQHSHPEVLRRMKRPASGERNLE
CCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHH
RIQRWREVCPEIVIRSSFIVGFPGETQAEFEHLLDFLRAARIDRVGCFAYSDVSGAVAND
HHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCHHEEEEECCCCCHHHHC
LPGMLPMPLRQERRARFMAVAEALSSAKLQRRVGATMQVLIDAAPGLGRKGGVGRSYADA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEECCCCCCCCCCCCCCCCCC
PEIDGAVHLLPPEKISKTLKVGEFTQARIVGVRGHDLLAQPI
CCCCCEEEECCHHHHHHHHHCCCCCEEEEEEECCCCHHCCCC
>Mature Secondary Structure 
TTMTAERAPRIGMVSLGCPKALTDSELILTQLSAEGYETSKTFQGADLVIVNTCGFIDD
CCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHH
AVKESLDTIGEALAENGKVIVTGCLGARTGADGGNMVRQLHPSVLAVTGPQATQQVLDAV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHH
HRNLPKPHDPFIDLVPGGMGIAGLKLTPRHYAYLKISEGCNHRCTFCIIPTLRGALVSRP
HHCCCCCCCCCEEECCCCCCEECEEECCCEEEEEEECCCCCCCEEEEEECHHHHHHHHCH
IGAVLNEARALFAGGVKELLVISQDSSAYGVDMQYRTGFWDGQPLKTRLLELVQALGALA
HHHHHHHHHHHHHCCHHHEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
EPYGAWVRLHYVYPYPSVDALIPLMAQGRVLPYLDVPLQHSHPEVLRRMKRPASGERNLE
CCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHH
RIQRWREVCPEIVIRSSFIVGFPGETQAEFEHLLDFLRAARIDRVGCFAYSDVSGAVAND
HHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCHHEEEEECCCCCHHHHC
LPGMLPMPLRQERRARFMAVAEALSSAKLQRRVGATMQVLIDAAPGLGRKGGVGRSYADA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEECCCCCCCCCCCCCCCCCC
PEIDGAVHLLPPEKISKTLKVGEFTQARIVGVRGHDLLAQPI
CCCCCEEEECCHHHHHHHHHCCCCCEEEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA