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The map label for this gene is folA [H]

Identifier: 121610114

GI number: 121610114

Start: 3536193

End: 3536681

Strand: Direct

Name: folA [H]

Synonym: Veis_3173

Alternate gene names: 121610114

Gene position: 3536193-3536681 (Clockwise)

Preceding gene: 121610113

Following gene: 121610118

Centisome position: 63.52

GC content: 69.33

Gene sequence:

>489_bases
ATGCCGCTGCACCTGATCTACGCCCGCGCGGCCAACGGCGTCATCGGCAAGGACGGCCGCCTGCCCTGGCATCTGCCGCA
GGACATGGCGCATTTCAGGCAACTCACCCAGGGTTGCAGCGTGATCATGGGCCGCAAGACCTGGGACTCGCTGCCCGCGC
GCTTTCGCCCGCTGCCGGGCCGCAGCAACATCGTGGTGACGCGCCAAAGCGACTGGCATGCCGACGGCGCCCGGCGCGCA
GCCAGCCTGGAGCAAGCCCTGGCGCTCTGCGATGCCCGCCAGACCGCCTGGGTGATCGGCGGCGCGCAACTGTATGCCCA
AGCCCTGCCTCTGGCCGACTGCGTCGAGGTGACCGAGATCAACCAAGACTTCGACGGCGATGCCCACGCGCCCACACTGG
GTGCCGAGTGGGTCGAGATCCGGCGCAGCCGCCAGCCCGATCAGCACGGGCTGGCTTTCAGTTTTGTGCGCTATGCGCGC
GCGGGCTGA

Upstream 100 bases:

>100_bases
CGGCCCGACAGCATCTTCGACTACCGCTACGAAGATTTCGAGGTGCTGGACTACCGATGCCACCCCGCGATCAAGGCGCC
CGTGGCGGTCTGACCGGGTC

Downstream 100 bases:

>100_bases
CCACCTCCTGCCAGCACAGGGCCCCTGTGGCCACCGTGGCGAGGGCACTGGACATGGGCGCGATGCTCTGCCCGAACGCA
CCACCCCCTTCAATCATCTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MPLHLIYARAANGVIGKDGRLPWHLPQDMAHFRQLTQGCSVIMGRKTWDSLPARFRPLPGRSNIVVTRQSDWHADGARRA
ASLEQALALCDARQTAWVIGGAQLYAQALPLADCVEVTEINQDFDGDAHAPTLGAEWVEIRRSRQPDQHGLAFSFVRYAR
AG

Sequences:

>Translated_162_residues
MPLHLIYARAANGVIGKDGRLPWHLPQDMAHFRQLTQGCSVIMGRKTWDSLPARFRPLPGRSNIVVTRQSDWHADGARRA
ASLEQALALCDARQTAWVIGGAQLYAQALPLADCVEVTEINQDFDGDAHAPTLGAEWVEIRRSRQPDQHGLAFSFVRYAR
AG
>Mature_161_residues
PLHLIYARAANGVIGKDGRLPWHLPQDMAHFRQLTQGCSVIMGRKTWDSLPARFRPLPGRSNIVVTRQSDWHADGARRAA
SLEQALALCDARQTAWVIGGAQLYAQALPLADCVEVTEINQDFDGDAHAPTLGAEWVEIRRSRQPDQHGLAFSFVRYARA
G

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=181, Percent_Identity=32.5966850828729, Blast_Score=86, Evalue=1e-17,
Organism=Homo sapiens, GI28827793, Length=151, Percent_Identity=35.0993377483444, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1786233, Length=129, Percent_Identity=40.3100775193798, Blast_Score=94, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17505899, Length=181, Percent_Identity=37.0165745856354, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324810, Length=124, Percent_Identity=37.0967741935484, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI116008026, Length=172, Percent_Identity=30.2325581395349, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24647458, Length=172, Percent_Identity=30.2325581395349, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17968; Mature: 17836

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHLIYARAANGVIGKDGRLPWHLPQDMAHFRQLTQGCSVIMGRKTWDSLPARFRPLPG
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCHHHCCHHCCCCCC
RSNIVVTRQSDWHADGARRAASLEQALALCDARQTAWVIGGAQLYAQALPLADCVEVTEI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCHHHHHHHHHC
NQDFDGDAHAPTLGAEWVEIRRSRQPDQHGLAFSFVRYARAG
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PLHLIYARAANGVIGKDGRLPWHLPQDMAHFRQLTQGCSVIMGRKTWDSLPARFRPLPG
CEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCHHHCCHHCCCCCC
RSNIVVTRQSDWHADGARRAASLEQALALCDARQTAWVIGGAQLYAQALPLADCVEVTEI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCHHHHHHHHHC
NQDFDGDAHAPTLGAEWVEIRRSRQPDQHGLAFSFVRYARAG
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]