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The map label for this gene is thyA [H]

Identifier: 121610113

GI number: 121610113

Start: 3535289

End: 3536185

Strand: Direct

Name: thyA [H]

Synonym: Veis_3172

Alternate gene names: 121610113

Gene position: 3535289-3536185 (Clockwise)

Preceding gene: 121610090

Following gene: 121610114

Centisome position: 63.51

GC content: 64.66

Gene sequence:

>897_bases
ATGAACCAGTCCGCCCTTGCCCGCCCTGCGAGCACCCAGTGCGACAGCCCCGGGCGCGATCGGCCTGAGGCGCTCTTTCC
CCCCACCCCGTACGAGGACTTGATGCGCCATGTATTCAGGCATGGCGTGCACAAGTCGGACCGCACCGGCACCGGCACCA
AAAGCCTGTTCGGCCACCAGATGCGCTTTGATCTGCGCGGGGGCTTTCCGCTGGTCACCACGAAGAGAGTGCACCTCAAA
TCCATCATCGAAGAACTGCTGTGGTTCCTCGCCGGCTCGGGCAACAACCGCTGGCTGCAGCAGCGCGGTGTCAGCATTTG
GGACGAATGGGCGCGCGCAGACGGCGATCTGGGCCCGGTGTACGGCGTGCAATGGCGCAGTTGGCCCCGACCCGATGGCG
GGCATATCGACCAGATCGGTTCGGTCATCGCCACGCTCAAGACCAACCCCGACTCGCGCCGCATCATCGTCAGCGCCTGG
AACGTGGCCGAACTCGACAAAATGGCCTTGCAGCCCTGCCACATACTCTTTCAGTTCTACGTGGCCCCGGCGCAGACGCC
GGGCGGGCGCGGCACGCTCAGTTGCCAGCTCTACCAGCGCAGCGCCGACATCTTCCTGGGCCTGCCCTTCAACATCGCCA
GCTACGCGCTGCTGACCCATATGGTGGCCCAGCAATGCGAGTTGGACGTGGGTGAGTTCATCTGGACCGGTGGCGACTGC
CATATCTACGACAACCACCACGCTCAGGTGCAGACCCAACTGGCGCGCAGCCCCTACCCCTACCCGCAGTTGCGCATCAG
GCGCCGGCCCGACAGCATCTTCGACTACCGCTACGAAGATTTCGAGGTGCTGGACTACCGATGCCACCCCGCGATCAAGG
CGCCCGTGGCGGTCTGA

Upstream 100 bases:

>100_bases
CGCTCGCACGCTGTCCGCAGCGCGCACCGGGGCGCATAGAATCCGGCGATCGGCCTCGTGCCAATCCCCCTCCTTGCCCT
GCGATCGGCCCTTTTGCTCC

Downstream 100 bases:

>100_bases
CCGGGTCATGCCGCTGCACCTGATCTACGCCCGCGCGGCCAACGGCGTCATCGGCAAGGACGGCCGCCTGCCCTGGCATC
TGCCGCAGGACATGGCGCAT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNQSALARPASTQCDSPGRDRPEALFPPTPYEDLMRHVFRHGVHKSDRTGTGTKSLFGHQMRFDLRGGFPLVTTKRVHLK
SIIEELLWFLAGSGNNRWLQQRGVSIWDEWARADGDLGPVYGVQWRSWPRPDGGHIDQIGSVIATLKTNPDSRRIIVSAW
NVAELDKMALQPCHILFQFYVAPAQTPGGRGTLSCQLYQRSADIFLGLPFNIASYALLTHMVAQQCELDVGEFIWTGGDC
HIYDNHHAQVQTQLARSPYPYPQLRIRRRPDSIFDYRYEDFEVLDYRCHPAIKAPVAV

Sequences:

>Translated_298_residues
MNQSALARPASTQCDSPGRDRPEALFPPTPYEDLMRHVFRHGVHKSDRTGTGTKSLFGHQMRFDLRGGFPLVTTKRVHLK
SIIEELLWFLAGSGNNRWLQQRGVSIWDEWARADGDLGPVYGVQWRSWPRPDGGHIDQIGSVIATLKTNPDSRRIIVSAW
NVAELDKMALQPCHILFQFYVAPAQTPGGRGTLSCQLYQRSADIFLGLPFNIASYALLTHMVAQQCELDVGEFIWTGGDC
HIYDNHHAQVQTQLARSPYPYPQLRIRRRPDSIFDYRYEDFEVLDYRCHPAIKAPVAV
>Mature_298_residues
MNQSALARPASTQCDSPGRDRPEALFPPTPYEDLMRHVFRHGVHKSDRTGTGTKSLFGHQMRFDLRGGFPLVTTKRVHLK
SIIEELLWFLAGSGNNRWLQQRGVSIWDEWARADGDLGPVYGVQWRSWPRPDGGHIDQIGSVIATLKTNPDSRRIIVSAW
NVAELDKMALQPCHILFQFYVAPAQTPGGRGTLSCQLYQRSADIFLGLPFNIASYALLTHMVAQQCELDVGEFIWTGGDC
HIYDNHHAQVQTQLARSPYPYPQLRIRRRPDSIFDYRYEDFEVLDYRCHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=288, Percent_Identity=50.3472222222222, Blast_Score=271, Evalue=5e-73,
Organism=Escherichia coli, GI1789191, Length=268, Percent_Identity=66.7910447761194, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71993377, Length=289, Percent_Identity=44.9826989619377, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6324648, Length=278, Percent_Identity=41.726618705036, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI17137556, Length=317, Percent_Identity=47.3186119873817, Blast_Score=285, Evalue=2e-77,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 33886; Mature: 33886

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSALARPASTQCDSPGRDRPEALFPPTPYEDLMRHVFRHGVHKSDRTGTGTKSLFGHQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCE
MRFDLRGGFPLVTTKRVHLKSIIEELLWFLAGSGNNRWLQQRGVSIWDEWARADGDLGPV
EEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCCCC
YGVQWRSWPRPDGGHIDQIGSVIATLKTNPDSRRIIVSAWNVAELDKMALQPCHILFQFY
CCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
VAPAQTPGGRGTLSCQLYQRSADIFLGLPFNIASYALLTHMVAQQCELDVGEFIWTGGDC
HCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCEE
HIYDNHHAQVQTQLARSPYPYPQLRIRRRPDSIFDYRYEDFEVLDYRCHPAIKAPVAV
EEECCCCHHHHHHHHCCCCCCCCCEEECCCCHHHCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNQSALARPASTQCDSPGRDRPEALFPPTPYEDLMRHVFRHGVHKSDRTGTGTKSLFGHQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCE
MRFDLRGGFPLVTTKRVHLKSIIEELLWFLAGSGNNRWLQQRGVSIWDEWARADGDLGPV
EEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCCCC
YGVQWRSWPRPDGGHIDQIGSVIATLKTNPDSRRIIVSAWNVAELDKMALQPCHILFQFY
CCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
VAPAQTPGGRGTLSCQLYQRSADIFLGLPFNIASYALLTHMVAQQCELDVGEFIWTGGDC
HCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCEE
HIYDNHHAQVQTQLARSPYPYPQLRIRRRPDSIFDYRYEDFEVLDYRCHPAIKAPVAV
EEECCCCHHHHHHHHCCCCCCCCCEEECCCCHHHCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA