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The map label for this gene is galE [C]

Identifier: 121610080

GI number: 121610080

Start: 3489345

End: 3490415

Strand: Direct

Name: galE [C]

Synonym: Veis_3138

Alternate gene names: 121610080

Gene position: 3489345-3490415 (Clockwise)

Preceding gene: 121610077

Following gene: 121610081

Centisome position: 62.68

GC content: 70.4

Gene sequence:

>1071_bases
ATGGCCTGCCGCCATGAATCGGATACAACAGCACCATTTCCCGGAAAAAGAGAGCACCCCATGCACATCCTCATCACCGG
CGGCTGCGGCTTTCTCGGCGTGCGGCTGGCGCGCACGCTGCTGGCCGGCGGCCCGCTGGCACTGGCCGGAGCACCGGCCC
GGGCCATCGCGCGCATCACGTTGGCCGACCGCGTGCCGCCGCCGGCCGACCTGCAAGCCGACGCCCGCGTGCAGTTCGTG
CCGGGCGATCTGTGCGAGCAGGTGGACAACGGTGCACTGCCGCTGGCGCGCGCCGACGCCTTGTTCCACCTGGCCGCAGC
GGTGAGCGGCGAGTGCGAGGCCGACTTCGACCTCGGCATGCGCAGCAACCTCAATGCCACGCGCATGTTGCTGCAAGCCT
GCCGCCATGCGGGCCATGCGCCGGTGTTCGTGTTTTCCAGCTCGGTCGCGGTCTTTGGCGGCTCGCCCGAGCAACCGCTG
CCGGCAGTGATCGAGGACAGCACGCTGCCGACGCCGCAAAGCAGCTATGGCATCCAGAAGTTCATCGGCGAGCAGTTGGT
GGCCGACTTCAGCCGCAAGGGCTTTGTGCAGGGCCGCAGTGTGCGGCTGATGACGGTGTCGGTGCGGCCGGGCCGTCCGA
ACGGCGCGGCCTCGGGGTTCCTCAGCGGCATGTTGCGCGAGCCTTTGGCCGGCGAGCGCGCGCGCTGCCCGGTGGCCCCC
GAGACGCCGGTCGCGCTCGCCTCGCCCGGCAACACCGTCGCCGGCATCATCCGTGCGGCCAGCGCCAGCGCCGCCGAATG
GCGCGTGCGCACCGCCGTCAACCTGCCCGCGCTCACCACCACCGTGCGCGAGATGGCGCAGGCGCTCGAACATGTCGCGG
GCCGCGCCACCAGCGCGCTGATCGACTGGGAGCCCGATGCGCAGATCGCCAAGATCGTTACCAGTTGGCCCAGCCGCATC
CACGCCGCGCGCGCTCAGTCCTTGGGCCTGAGCGCCGACCCGAGCTTTGACGCCATCTTGCGCAGCTACCTGCGCGAGAA
CCCGCAGGCGCACCAATGGCCAAGGCAATGA

Upstream 100 bases:

>100_bases
GCGGGCGAACGCTTGATTGTCGCCATCGGCGCTGCGGGTTTTGCCGTGGCCAGGCCCGAGCGGCGCAAGAATTTGTTTGA
CTATTTGTTTGACTTTGGCC

Downstream 100 bases:

>100_bases
CCCCGCTGTGTGCGTTGTGCGTTGCTGGCGCGGGCCGCGGCATGGCGCCCTTGCGCTGCGCCGGCGGATACCCGGTGGAT
GCCTGGCTCGACGCCCGAAT

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MACRHESDTTAPFPGKREHPMHILITGGCGFLGVRLARTLLAGGPLALAGAPARAIARITLADRVPPPADLQADARVQFV
PGDLCEQVDNGALPLARADALFHLAAAVSGECEADFDLGMRSNLNATRMLLQACRHAGHAPVFVFSSSVAVFGGSPEQPL
PAVIEDSTLPTPQSSYGIQKFIGEQLVADFSRKGFVQGRSVRLMTVSVRPGRPNGAASGFLSGMLREPLAGERARCPVAP
ETPVALASPGNTVAGIIRAASASAAEWRVRTAVNLPALTTTVREMAQALEHVAGRATSALIDWEPDAQIAKIVTSWPSRI
HAARAQSLGLSADPSFDAILRSYLRENPQAHQWPRQ

Sequences:

>Translated_356_residues
MACRHESDTTAPFPGKREHPMHILITGGCGFLGVRLARTLLAGGPLALAGAPARAIARITLADRVPPPADLQADARVQFV
PGDLCEQVDNGALPLARADALFHLAAAVSGECEADFDLGMRSNLNATRMLLQACRHAGHAPVFVFSSSVAVFGGSPEQPL
PAVIEDSTLPTPQSSYGIQKFIGEQLVADFSRKGFVQGRSVRLMTVSVRPGRPNGAASGFLSGMLREPLAGERARCPVAP
ETPVALASPGNTVAGIIRAASASAAEWRVRTAVNLPALTTTVREMAQALEHVAGRATSALIDWEPDAQIAKIVTSWPSRI
HAARAQSLGLSADPSFDAILRSYLRENPQAHQWPRQ
>Mature_355_residues
ACRHESDTTAPFPGKREHPMHILITGGCGFLGVRLARTLLAGGPLALAGAPARAIARITLADRVPPPADLQADARVQFVP
GDLCEQVDNGALPLARADALFHLAAAVSGECEADFDLGMRSNLNATRMLLQACRHAGHAPVFVFSSSVAVFGGSPEQPLP
AVIEDSTLPTPQSSYGIQKFIGEQLVADFSRKGFVQGRSVRLMTVSVRPGRPNGAASGFLSGMLREPLAGERARCPVAPE
TPVALASPGNTVAGIIRAASASAAEWRVRTAVNLPALTTTVREMAQALEHVAGRATSALIDWEPDAQIAKIVTSWPSRIH
AARAQSLGLSADPSFDAILRSYLRENPQAHQWPRQ

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37657; Mature: 37526

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACRHESDTTAPFPGKREHPMHILITGGCGFLGVRLARTLLAGGPLALAGAPARAIARIT
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCHHHHEEEH
LADRVPPPADLQADARVQFVPGDLCEQVDNGALPLARADALFHLAAAVSGECEADFDLGM
HHCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
RSNLNATRMLLQACRHAGHAPVFVFSSSVAVFGGSPEQPLPAVIEDSTLPTPQSSYGIQK
CCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCHHCCCCCCCCCCCHHHHHH
FIGEQLVADFSRKGFVQGRSVRLMTVSVRPGRPNGAASGFLSGMLREPLAGERARCPVAP
HHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
ETPVALASPGNTVAGIIRAASASAAEWRVRTAVNLPALTTTVREMAQALEHVAGRATSAL
CCCEEEECCCCHHHHHHHHHCCCCHHEEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHEE
IDWEPDAQIAKIVTSWPSRIHAARAQSLGLSADPSFDAILRSYLRENPQAHQWPRQ
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ACRHESDTTAPFPGKREHPMHILITGGCGFLGVRLARTLLAGGPLALAGAPARAIARIT
CCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCHHHHEEEH
LADRVPPPADLQADARVQFVPGDLCEQVDNGALPLARADALFHLAAAVSGECEADFDLGM
HHCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
RSNLNATRMLLQACRHAGHAPVFVFSSSVAVFGGSPEQPLPAVIEDSTLPTPQSSYGIQK
CCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCHHCCCCCCCCCCCHHHHHH
FIGEQLVADFSRKGFVQGRSVRLMTVSVRPGRPNGAASGFLSGMLREPLAGERARCPVAP
HHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
ETPVALASPGNTVAGIIRAASASAAEWRVRTAVNLPALTTTVREMAQALEHVAGRATSAL
CCCEEEECCCCHHHHHHHHHCCCCHHEEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHEE
IDWEPDAQIAKIVTSWPSRIHAARAQSLGLSADPSFDAILRSYLRENPQAHQWPRQ
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]