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The map label for this gene is lcfB [H]

Identifier: 121609883

GI number: 121609883

Start: 3287265

End: 3288875

Strand: Direct

Name: lcfB [H]

Synonym: Veis_2936

Alternate gene names: 121609883

Gene position: 3287265-3288875 (Clockwise)

Preceding gene: 121609882

Following gene: 121609885

Centisome position: 59.05

GC content: 67.41

Gene sequence:

>1611_bases
ATGGCGGCCCCGAAATACCTCGGCGACTTCGCCGTCCTGACGCCGGACAAGCCGGCCGTCATCAACGGCACCACTGGCGC
GAGCCTGAGCTACCGCGAACTGGAGGAGCGCTCCAACCGGTTCGCGCAATACCTGTATGCGCTCGGCCTGCGGCGCGGCG
AGCGCATTGCGATGCTGCTCGAGAACCATCTGCGCTGCTTCGAGGTGGCGTGGGCGGCATTCCGTTCGGGCCTGTTGCTG
ACGGCGGTCAACCGCTACCTGACCGCCGACGAGGCGGCCTACATCATCACGGACAGCGATGCGCGCGTCGTCGTTTCCTC
GTTCGCGATGCGCGCTTTGGCGGCGCAGCTCAGTTGCGCCATGCCCGCTTGCGAGCGCCGCCTGATGCTCGACGGAACGA
TCCCGGGCTGGGACAGCTACGAGCAGGCCATTGCCGCCGCATCCGCTGCGCGCCTGGCCGACGAGTGGCTCGGCGCCACC
ATGTTCTACAGTTCCGGCACCACTGGCCGTCCCAAGGGCGTGGTGCGCGCGCAAAGGGCCTGCCGGATCGATGAGGCCCC
GGACCTGGACACACGCATGGCGATGATGCGCCACTACCTGTTCACGGCCGAATCGATATACCTCTCGCCGGCGCCGCTGT
ACCACGCGGCACCGCTGAACTACACGACCAACGTGCAGTTTTGCGGCGGCACGGTGGTCTTCATGGAGAAATTCGAGCCG
ATGCCGGCGCTGGCGCTGATCGATCGCTACCGCATCACCCACAGCCAATGGGTGCCGACGATGTTCATCCGGCTGCTCAA
GCTCTCACCCGGACAGCGCGCCGGATTCGATCTGCGCAGCCACCGCGTCGCCATCCACGCAGCCGCGCCCTGTCCGGTGG
AAGTCAAACGCCAGATGATCGAATGGTGGGGCCCGATCGTGCACGAGTACTACGGCGGCAGCGAGGGCGCCGGCCTCACC
GCCATCGACAGCCATGAAGCGCTCGCGCGGCCAGGCTCGGTCGGCAAGGCGTTGCAGGGCGTGATCCACATCTGCGCAGG
GGACGGGCAGGGCCGGCCATTGCCCGCCGGGCAGGACGGGCTGGTCTATTTCGAGCGCGACGCGCAGCCCTTCCACTACC
ACAAGGACCCCGAGAAGACGCGCGCCGCGCAGCACCCGTGCCACCCGTTGTGGTCGACCCTCGGCGACATCGGCCATCTC
GACGCGGACGGCTACCTGTATCTGACCGATCGCCAGTCCTTCATGATCATTTCGGGCGGGGTCAACATCTATCCGCAGGC
CATCGAGAACGCATTGGCGCTGCATCCGGGCGTAGCTGACGTGGCCGTGATCGGCGTGCCCGATCCGGAGATGGGCGAAG
CGGTCAAGGTCGTCATCGAGCCTGCCGCAGGCGTGCTGCCATCGGCCGCGCTGGCGGAAGAACTGCTGGCTTTCGTGCGC
GGCAAGGTCGCGCGATTCATGGTGCCGCGCTCGCTCGACTTCATCGACCAGATGCCGCGCCTGCCCACCGGCAAGCTCTA
CAAGCAGGTGCTGCGCGAACGCTACGCGAAAGCGCCGGGGCAGCCGGATCACCCCGATCACCCCGCCTCGCACACCGCCC
CCGCCGCCTAG

Upstream 100 bases:

>100_bases
CGAGCATGTGCGCACCAGCGTGGTCCAGGGCGTGGGCGGCATGTTCACCGCCGCAGCCACACTGGTGCTGTCCAACCAGA
GCGATTGAGGCGGCGACGCG

Downstream 100 bases:

>100_bases
TGTCGCGTCACCGATCATCTGTCGGTCTGCGCTGGCCATCGAAGCGCATCGCGGCGTTGCATCGCTTGCCAATACGCTCG
GTATGGGCTGCGCGATGCGC

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MAAPKYLGDFAVLTPDKPAVINGTTGASLSYRELEERSNRFAQYLYALGLRRGERIAMLLENHLRCFEVAWAAFRSGLLL
TAVNRYLTADEAAYIITDSDARVVVSSFAMRALAAQLSCAMPACERRLMLDGTIPGWDSYEQAIAAASAARLADEWLGAT
MFYSSGTTGRPKGVVRAQRACRIDEAPDLDTRMAMMRHYLFTAESIYLSPAPLYHAAPLNYTTNVQFCGGTVVFMEKFEP
MPALALIDRYRITHSQWVPTMFIRLLKLSPGQRAGFDLRSHRVAIHAAAPCPVEVKRQMIEWWGPIVHEYYGGSEGAGLT
AIDSHEALARPGSVGKALQGVIHICAGDGQGRPLPAGQDGLVYFERDAQPFHYHKDPEKTRAAQHPCHPLWSTLGDIGHL
DADGYLYLTDRQSFMIISGGVNIYPQAIENALALHPGVADVAVIGVPDPEMGEAVKVVIEPAAGVLPSAALAEELLAFVR
GKVARFMVPRSLDFIDQMPRLPTGKLYKQVLRERYAKAPGQPDHPDHPASHTAPAA

Sequences:

>Translated_536_residues
MAAPKYLGDFAVLTPDKPAVINGTTGASLSYRELEERSNRFAQYLYALGLRRGERIAMLLENHLRCFEVAWAAFRSGLLL
TAVNRYLTADEAAYIITDSDARVVVSSFAMRALAAQLSCAMPACERRLMLDGTIPGWDSYEQAIAAASAARLADEWLGAT
MFYSSGTTGRPKGVVRAQRACRIDEAPDLDTRMAMMRHYLFTAESIYLSPAPLYHAAPLNYTTNVQFCGGTVVFMEKFEP
MPALALIDRYRITHSQWVPTMFIRLLKLSPGQRAGFDLRSHRVAIHAAAPCPVEVKRQMIEWWGPIVHEYYGGSEGAGLT
AIDSHEALARPGSVGKALQGVIHICAGDGQGRPLPAGQDGLVYFERDAQPFHYHKDPEKTRAAQHPCHPLWSTLGDIGHL
DADGYLYLTDRQSFMIISGGVNIYPQAIENALALHPGVADVAVIGVPDPEMGEAVKVVIEPAAGVLPSAALAEELLAFVR
GKVARFMVPRSLDFIDQMPRLPTGKLYKQVLRERYAKAPGQPDHPDHPASHTAPAA
>Mature_535_residues
AAPKYLGDFAVLTPDKPAVINGTTGASLSYRELEERSNRFAQYLYALGLRRGERIAMLLENHLRCFEVAWAAFRSGLLLT
AVNRYLTADEAAYIITDSDARVVVSSFAMRALAAQLSCAMPACERRLMLDGTIPGWDSYEQAIAAASAARLADEWLGATM
FYSSGTTGRPKGVVRAQRACRIDEAPDLDTRMAMMRHYLFTAESIYLSPAPLYHAAPLNYTTNVQFCGGTVVFMEKFEPM
PALALIDRYRITHSQWVPTMFIRLLKLSPGQRAGFDLRSHRVAIHAAAPCPVEVKRQMIEWWGPIVHEYYGGSEGAGLTA
IDSHEALARPGSVGKALQGVIHICAGDGQGRPLPAGQDGLVYFERDAQPFHYHKDPEKTRAAQHPCHPLWSTLGDIGHLD
ADGYLYLTDRQSFMIISGGVNIYPQAIENALALHPGVADVAVIGVPDPEMGEAVKVVIEPAAGVLPSAALAEELLAFVRG
KVARFMVPRSLDFIDQMPRLPTGKLYKQVLRERYAKAPGQPDHPDHPASHTAPAA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=522, Percent_Identity=27.5862068965517, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI42544132, Length=510, Percent_Identity=25.6862745098039, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI122937307, Length=521, Percent_Identity=27.6391554702495, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI156151445, Length=544, Percent_Identity=24.8161764705882, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI157311624, Length=507, Percent_Identity=25.8382642998028, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI157311622, Length=507, Percent_Identity=25.8382642998028, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=25.7874015748031, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI58082049, Length=507, Percent_Identity=25.8382642998028, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI187761345, Length=537, Percent_Identity=27.1880819366853, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI187761343, Length=537, Percent_Identity=27.1880819366853, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI38524616, Length=541, Percent_Identity=24.0295748613678, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI8923896, Length=551, Percent_Identity=23.049001814882, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI253970489, Length=401, Percent_Identity=24.1895261845387, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI13375727, Length=556, Percent_Identity=23.021582733813, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788107, Length=547, Percent_Identity=27.4223034734918, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI221142682, Length=532, Percent_Identity=26.6917293233083, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693145, Length=513, Percent_Identity=25.9259259259259, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=23.2600732600733, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1786810, Length=532, Percent_Identity=25.187969924812, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786529, Length=553, Percent_Identity=23.3273056057866, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=27.3255813953488, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI32563687, Length=503, Percent_Identity=27.8330019880716, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71994694, Length=546, Percent_Identity=24.9084249084249, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=546, Percent_Identity=24.9084249084249, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71994690, Length=546, Percent_Identity=24.9084249084249, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=512, Percent_Identity=25, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=531, Percent_Identity=23.1638418079096, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=550, Percent_Identity=24.9090909090909, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=540, Percent_Identity=25.3703703703704, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=520, Percent_Identity=25.3846153846154, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319699, Length=543, Percent_Identity=23.7569060773481, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323182, Length=568, Percent_Identity=22.887323943662, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319264, Length=565, Percent_Identity=23.3628318584071, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355181, Length=523, Percent_Identity=25.0478011472275, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24581924, Length=548, Percent_Identity=24.8175182481752, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922652, Length=518, Percent_Identity=26.4478764478764, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24656500, Length=537, Percent_Identity=23.6499068901304, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21358303, Length=491, Percent_Identity=25.050916496945, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648255, Length=480, Percent_Identity=23.125, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648253, Length=481, Percent_Identity=23.0769230769231, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI281365686, Length=536, Percent_Identity=25.1865671641791, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI18859661, Length=516, Percent_Identity=24.031007751938, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI62472339, Length=541, Percent_Identity=23.4750462107209, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24667955, Length=541, Percent_Identity=23.4750462107209, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21356441, Length=528, Percent_Identity=22.1590909090909, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648260, Length=539, Percent_Identity=22.4489795918367, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130357, Length=531, Percent_Identity=23.728813559322, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667959, Length=385, Percent_Identity=24.9350649350649, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21355225, Length=385, Percent_Identity=24.6753246753247, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=535, Percent_Identity=20.3738317757009, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648257, Length=531, Percent_Identity=21.2806026365348, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI161076582, Length=548, Percent_Identity=20.2554744525547, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356947, Length=525, Percent_Identity=22.2857142857143, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 58693; Mature: 58562

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPKYLGDFAVLTPDKPAVINGTTGASLSYRELEERSNRFAQYLYALGLRRGERIAMLL
CCCCCCCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ENHLRCFEVAWAAFRSGLLLTAVNRYLTADEAAYIITDSDARVVVSSFAMRALAAQLSCA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
MPACERRLMLDGTIPGWDSYEQAIAAASAARLADEWLGATMFYSSGTTGRPKGVVRAQRA
CHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHH
CRIDEAPDLDTRMAMMRHYLFTAESIYLSPAPLYHAAPLNYTTNVQFCGGTVVFMEKFEP
HCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCC
MPALALIDRYRITHSQWVPTMFIRLLKLSPGQRAGFDLRSHRVAIHAAAPCPVEVKRQMI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHH
EWWGPIVHEYYGGSEGAGLTAIDSHEALARPGSVGKALQGVIHICAGDGQGRPLPAGQDG
HHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCC
LVYFERDAQPFHYHKDPEKTRAAQHPCHPLWSTLGDIGHLDADGYLYLTDRQSFMIISGG
EEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEECC
VNIYPQAIENALALHPGVADVAVIGVPDPEMGEAVKVVIEPAAGVLPSAALAEELLAFVR
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
GKVARFMVPRSLDFIDQMPRLPTGKLYKQVLRERYAKAPGQPDHPDHPASHTAPAA
HHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAPKYLGDFAVLTPDKPAVINGTTGASLSYRELEERSNRFAQYLYALGLRRGERIAMLL
CCCCCCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ENHLRCFEVAWAAFRSGLLLTAVNRYLTADEAAYIITDSDARVVVSSFAMRALAAQLSCA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
MPACERRLMLDGTIPGWDSYEQAIAAASAARLADEWLGATMFYSSGTTGRPKGVVRAQRA
CHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHH
CRIDEAPDLDTRMAMMRHYLFTAESIYLSPAPLYHAAPLNYTTNVQFCGGTVVFMEKFEP
HCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCC
MPALALIDRYRITHSQWVPTMFIRLLKLSPGQRAGFDLRSHRVAIHAAAPCPVEVKRQMI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHH
EWWGPIVHEYYGGSEGAGLTAIDSHEALARPGSVGKALQGVIHICAGDGQGRPLPAGQDG
HHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCC
LVYFERDAQPFHYHKDPEKTRAAQHPCHPLWSTLGDIGHLDADGYLYLTDRQSFMIISGG
EEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEECC
VNIYPQAIENALALHPGVADVAVIGVPDPEMGEAVKVVIEPAAGVLPSAALAEELLAFVR
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
GKVARFMVPRSLDFIDQMPRLPTGKLYKQVLRERYAKAPGQPDHPDHPASHTAPAA
HHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]