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The map label for this gene is purT

Identifier: 120612894

GI number: 120612894

Start: 4721452

End: 4722696

Strand: Reverse

Name: purT

Synonym: Aave_4258

Alternate gene names: 120612894

Gene position: 4722696-4721452 (Counterclockwise)

Preceding gene: 120612898

Following gene: 120612893

Centisome position: 88.23

GC content: 70.92

Gene sequence:

>1245_bases
ATGCCCATCACTCTCGGCACCCCCCTCTCGCCTTCCGCCACCAAAGTCATGCTGCTCGGCTCCGGCGAGCTGGGCAAGGA
AGTGCTCATCGCCCTGCATCGCCTGGGCGTGGAAACCACGGCGGTGGACCGCTACGACAACGCGCCCGGCCAGCAGGTGG
CCCACCATGCGCGCACCATCACCATGAGCGATCCGGCGCAGCTGAAAGCGCTGATCGAGGCCGAGCGCCCGCACCTGGTC
GTGCCCGAGATCGAAGCCATCGCCACGCCCATGCTCGAAGAGCTGGAGGCCGCCGGCACCGTGCGCGTGATCCCCACGGC
CCGCGCCGCGCGCCTCACCATGGACCGCGAAGGCATCCGCCGCCTGGCCGCGGAGACGCTGGGCCTGCCCACCAGCCCCT
ACCGCTTCTGCGATTCGGCCGAGGAACTGCAGGCCGCCATCGACGGCACCGACGGCCAGCCCGCCATCGGTTTTCCCTGC
GTGGTGAAGCCGGTGATGAGCAGCTCCGGCAAGGGCCAGAGCAAGCTGGACGGCCCGGCCGACGTGCAAAAGGCCTGGGA
CTACGCCATGGCCGGCGGCCGTGTGAGCCATGGCCGTGTGATCGTCGAGGGCTTCATCGATTTCGAATACGAGATCACCC
TGCTCACCGTGCGCGCCCGCGCAGCCGACGGCAGCATCCAGACATCCTTCTGCGAGCCCATCGGCCACAAGCAGGTCAGC
GGCGACTACGTGGAAAGCTGGCAGCCCCAGCCCATGCCGCCGCTCGCGCTGCAGCGCGCCAGGGACATCGCCAGGGCCGT
GACGGACGACCTGGGTCGCGGCCTGGACGGCCAGCCCTCGGGCCAGGGACTGTTCGGCGTGGAACTGTTCGTGAAGGGCG
ACGAGGTCTGGTTCAGCGAAGTCAGCCCGCGCCCGCACGACACCGGCCTGGTCACCCTGGCCACGCAGCACCAGAGCGAA
TTCGAGCTGCACGCCCGGGCCATCCTGGGCCTGCCCGTCGATACGTCGCTGCGCAACCCCGGCGCCAGCGCCGTGATCTA
CGGCGGCGTCGATGCGCAAGGCATCGCCTTCGACGGCGTCGATGAAGCCCTGGCCGTGCCGGGCACCGACCTGCGCCTCT
TCGGCAAGCCCGAGAGCTTCGCCCGGCGCCGCATGGGCGTGGCCGTGGCCCGGGCGGACGACGTGGACACGGCGCGCGCC
AATGCGCGGCTGGCGGCGGGCAGGGTGAGGCCGCGCGCGGCGTGA

Upstream 100 bases:

>100_bases
AGGTGGGCGATCTCGAAAAGCGGGGAGGATGTGGCCATGGCGTCTATTATTGGCCGCCCTTTCGCCTTCCCCTTCTCCTT
CACCTTCCTTCGACGATCAC

Downstream 100 bases:

>100_bases
GCGGCTGGCGGCTGCCGGCCAGCCCGCGGCACCGCCCCCCACGCGTGGCTGTCAGCGAACCGACTTAAAAGTCGCAGGGT
GCAACAGCGATGTGCATCCG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MPITLGTPLSPSATKVMLLGSGELGKEVLIALHRLGVETTAVDRYDNAPGQQVAHHARTITMSDPAQLKALIEAERPHLV
VPEIEAIATPMLEELEAAGTVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYRFCDSAEELQAAIDGTDGQPAIGFPC
VVKPVMSSSGKGQSKLDGPADVQKAWDYAMAGGRVSHGRVIVEGFIDFEYEITLLTVRARAADGSIQTSFCEPIGHKQVS
GDYVESWQPQPMPPLALQRARDIARAVTDDLGRGLDGQPSGQGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLATQHQSE
FELHARAILGLPVDTSLRNPGASAVIYGGVDAQGIAFDGVDEALAVPGTDLRLFGKPESFARRRMGVAVARADDVDTARA
NARLAAGRVRPRAA

Sequences:

>Translated_414_residues
MPITLGTPLSPSATKVMLLGSGELGKEVLIALHRLGVETTAVDRYDNAPGQQVAHHARTITMSDPAQLKALIEAERPHLV
VPEIEAIATPMLEELEAAGTVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYRFCDSAEELQAAIDGTDGQPAIGFPC
VVKPVMSSSGKGQSKLDGPADVQKAWDYAMAGGRVSHGRVIVEGFIDFEYEITLLTVRARAADGSIQTSFCEPIGHKQVS
GDYVESWQPQPMPPLALQRARDIARAVTDDLGRGLDGQPSGQGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLATQHQSE
FELHARAILGLPVDTSLRNPGASAVIYGGVDAQGIAFDGVDEALAVPGTDLRLFGKPESFARRRMGVAVARADDVDTARA
NARLAAGRVRPRAA
>Mature_413_residues
PITLGTPLSPSATKVMLLGSGELGKEVLIALHRLGVETTAVDRYDNAPGQQVAHHARTITMSDPAQLKALIEAERPHLVV
PEIEAIATPMLEELEAAGTVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYRFCDSAEELQAAIDGTDGQPAIGFPCV
VKPVMSSSGKGQSKLDGPADVQKAWDYAMAGGRVSHGRVIVEGFIDFEYEITLLTVRARAADGSIQTSFCEPIGHKQVSG
DYVESWQPQPMPPLALQRARDIARAVTDDLGRGLDGQPSGQGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLATQHQSEF
ELHARAILGLPVDTSLRNPGASAVIYGGVDAQGIAFDGVDEALAVPGTDLRLFGKPESFARRRMGVAVARADDVDTARAN
ARLAAGRVRPRAA

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=409, Percent_Identity=56.9682151589242, Blast_Score=417, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6324702, Length=383, Percent_Identity=25.5874673629243, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_ACIAC (A1TV10)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_972572.1
- ProteinModelPortal:   A1TV10
- SMR:   A1TV10
- STRING:   A1TV10
- GeneID:   4667511
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_4258
- NMPDR:   fig|397945.5.peg.3655
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   A1TV10
- ProtClustDB:   PRK09288
- BioCyc:   AAVE397945:AAVE_4258-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44130; Mature: 43998

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 83-83 BINDING 116-116 BINDING 163-163 BINDING 211-211 BINDING 288-288 BINDING 300-300 BINDING 307-307 BINDING 376-376

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITLGTPLSPSATKVMLLGSGELGKEVLIALHRLGVETTAVDRYDNAPGQQVAHHARTI
CCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHEEE
TMSDPAQLKALIEAERPHLVVPEIEAIATPMLEELEAAGTVRVIPTARAARLTMDREGIR
EECCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHEECCHHHHH
RLAAETLGLPTSPYRFCDSAEELQAAIDGTDGQPAIGFPCVVKPVMSSSGKGQSKLDGPA
HHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVQKAWDYAMAGGRVSHGRVIVEGFIDFEYEITLLTVRARAADGSIQTSFCEPIGHKQVS
HHHHHHHHHHCCCEECCCEEEEEEEECEEEEEEEEEEEEECCCCCCCHHHHCCCCCCCCC
GDYVESWQPQPMPPLALQRARDIARAVTDDLGRGLDGQPSGQGLFGVELFVKGDEVWFSE
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCEEEEEC
VSPRPHDTGLVTLATQHQSEFELHARAILGLPVDTSLRNPGASAVIYGGVDAQGIAFDGV
CCCCCCCCCEEEEEECCCCHHHEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEECCC
DEALAVPGTDLRLFGKPESFARRRMGVAVARADDVDTARANARLAAGRVRPRAA
CHHHCCCCCCEEEECCCHHHHHHHHCEEEEECCCCCHHHCCCEEECCCCCCCCC
>Mature Secondary Structure 
PITLGTPLSPSATKVMLLGSGELGKEVLIALHRLGVETTAVDRYDNAPGQQVAHHARTI
CEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHEEE
TMSDPAQLKALIEAERPHLVVPEIEAIATPMLEELEAAGTVRVIPTARAARLTMDREGIR
EECCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHEECCHHHHH
RLAAETLGLPTSPYRFCDSAEELQAAIDGTDGQPAIGFPCVVKPVMSSSGKGQSKLDGPA
HHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVQKAWDYAMAGGRVSHGRVIVEGFIDFEYEITLLTVRARAADGSIQTSFCEPIGHKQVS
HHHHHHHHHHCCCEECCCEEEEEEEECEEEEEEEEEEEEECCCCCCCHHHHCCCCCCCCC
GDYVESWQPQPMPPLALQRARDIARAVTDDLGRGLDGQPSGQGLFGVELFVKGDEVWFSE
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCEEEEEC
VSPRPHDTGLVTLATQHQSEFELHARAILGLPVDTSLRNPGASAVIYGGVDAQGIAFDGV
CCCCCCCCCEEEEEECCCCHHHEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEECCC
DEALAVPGTDLRLFGKPESFARRRMGVAVARADDVDTARANARLAAGRVRPRAA
CHHHCCCCCCEEEECCCHHHHHHHHCEEEEECCCCCHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA