Click here to switch to the map view.

The map label for this gene is cca

Identifier: 120612844

GI number: 120612844

Start: 4663100

End: 4664359

Strand: Direct

Name: cca

Synonym: Aave_4208

Alternate gene names: 120612844

Gene position: 4663100-4664359 (Clockwise)

Preceding gene: 120612842

Following gene: 120612846

Centisome position: 87.12

GC content: 73.25

Gene sequence:

>1260_bases
ATGCAGATCTACATGGTGGGCGGCGCGGTGCGCGACCGGCTTCTGGGCCGCCCGGTGAACGACCGCGACTGGGTGGTGGT
GGGCGCCACGCCCGACGACCTGGCCGCGCGCGGCTTCCTGCCCGTGGGGCGCGATTTTCCCGTCTTCCTCCACCCCGAAA
CGCGCGAGGAATACGCGCTGGCGCGCACCGAGCGCAAGAGCGGGCGGGGCTACCGTGGCTTCGTGGTGGACACGGCCCCG
GACGTGACGCTGGAGCAGGACCTGTCGCGCCGCGATCTCACCATCAACGCGATGGCGGTACGCGGCGAAGACCCTGCGGC
ATGGGAACTGGTCGATCCATACGGCGGCGCGCGCGACCTGCAGGCGAAGCTGCTGCGCCACGTGACCGACGCATTCCGCG
AAGACCCGGTACGCATCCTGCGCGTGGCGCGCTTCGCCGCGCGCTTCACCGATTTCTCCGTCGCTCCGGAAACCATGGCG
CTCATGCGGGAGATGGTCGATGCCGGCGAGGCGGCCGACCTCGTTCCCGAGCGCGTCTGGCAGGAGATCGCGCGCGGCCT
GATGGAAGCCGCCCCGTCGCGCATGTTCGAGGTGCTGCGCGGCTGCGGCGCCCTGGCGGTGCTGCTGCCCGAGCTGGACC
GGCTCTGGGGCGTTCCGCAACGCGCCGAATACCATCCCGAGGTGGACACCGGCGTGCACGTGATGATGGTGCTGGACATG
GCCGCGCGGCTGCATGCACCGCTCGCCGTGCGCTTCGCCAGCCTCGTCCACGACCTGGGCAAGGGCACGACGCCGGCCGA
CGTGCTGCCGCGCCATATCGGGCACGAGCAGCGCAGCGCCACCCTGCTGGCCGATGTGTGCGAGCGCCTGCGGGTGCCCG
TGGAATGCCGCGAGACCGCCGACGTGGTCGCGCGCGAGCACGGAAACATCCACCGCAGCGCGGAGCTGGGCGCAGCGGCC
CTGGTGCGGCTGCTGGAGCGCTGCGACGCGCTGCGCAAGCCCGCCCGTTTCGCCGACGTGCTGCTGGCCTGCGAGTGCGA
CGCGCGCGGCCGGCTGGGATTCGAGGAGACGCCTTATCCGCAGCGCCAACGCCTGCTGGGCGCGCTGCAGGCGGCACGCT
CCGTCGATACCGCCGCCGTGGCCGCGCGGGCCCAGGCCCGCGGCGCCGCCGGCCCGCAGGTGGGCGAATGGATCCGCCGG
GCGCGCGGCGACGCGGTGCAGGAGTGGATGGCGGCGCAGCCGCCCGCGGCCGGAACCTGA

Upstream 100 bases:

>100_bases
TGGCAGACGGGCAGGAAGGATCGCTGCCGAATATACCGGCCGGTGCCTGCGCAGGCGCAGCGCATGCAGGAACGCCCGCC
CGCCGCCGTACACTGCCCGC

Downstream 100 bases:

>100_bases
CGGCCCCGTGCCGCGGGCCCGCGGCCTCAGGCGCCGCCGGCCGTCCGCACCCAGCGCGCGATGTCGGCGGCGCCCATCGC
CCCCGAGATGCGCGCGACTT

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MQIYMVGGAVRDRLLGRPVNDRDWVVVGATPDDLAARGFLPVGRDFPVFLHPETREEYALARTERKSGRGYRGFVVDTAP
DVTLEQDLSRRDLTINAMAVRGEDPAAWELVDPYGGARDLQAKLLRHVTDAFREDPVRILRVARFAARFTDFSVAPETMA
LMREMVDAGEAADLVPERVWQEIARGLMEAAPSRMFEVLRGCGALAVLLPELDRLWGVPQRAEYHPEVDTGVHVMMVLDM
AARLHAPLAVRFASLVHDLGKGTTPADVLPRHIGHEQRSATLLADVCERLRVPVECRETADVVAREHGNIHRSAELGAAA
LVRLLERCDALRKPARFADVLLACECDARGRLGFEETPYPQRQRLLGALQAARSVDTAAVAARAQARGAAGPQVGEWIRR
ARGDAVQEWMAAQPPAAGT

Sequences:

>Translated_419_residues
MQIYMVGGAVRDRLLGRPVNDRDWVVVGATPDDLAARGFLPVGRDFPVFLHPETREEYALARTERKSGRGYRGFVVDTAP
DVTLEQDLSRRDLTINAMAVRGEDPAAWELVDPYGGARDLQAKLLRHVTDAFREDPVRILRVARFAARFTDFSVAPETMA
LMREMVDAGEAADLVPERVWQEIARGLMEAAPSRMFEVLRGCGALAVLLPELDRLWGVPQRAEYHPEVDTGVHVMMVLDM
AARLHAPLAVRFASLVHDLGKGTTPADVLPRHIGHEQRSATLLADVCERLRVPVECRETADVVAREHGNIHRSAELGAAA
LVRLLERCDALRKPARFADVLLACECDARGRLGFEETPYPQRQRLLGALQAARSVDTAAVAARAQARGAAGPQVGEWIRR
ARGDAVQEWMAAQPPAAGT
>Mature_419_residues
MQIYMVGGAVRDRLLGRPVNDRDWVVVGATPDDLAARGFLPVGRDFPVFLHPETREEYALARTERKSGRGYRGFVVDTAP
DVTLEQDLSRRDLTINAMAVRGEDPAAWELVDPYGGARDLQAKLLRHVTDAFREDPVRILRVARFAARFTDFSVAPETMA
LMREMVDAGEAADLVPERVWQEIARGLMEAAPSRMFEVLRGCGALAVLLPELDRLWGVPQRAEYHPEVDTGVHVMMVLDM
AARLHAPLAVRFASLVHDLGKGTTPADVLPRHIGHEQRSATLLADVCERLRVPVECRETADVVAREHGNIHRSAELGAAA
LVRLLERCDALRKPARFADVLLACECDARGRLGFEETPYPQRQRLLGALQAARSVDTAAVAARAQARGAAGPQVGEWIRR
ARGDAVQEWMAAQPPAAGT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=417, Percent_Identity=54.1966426858513, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI87081691, Length=228, Percent_Identity=29.8245614035088, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_ACIAC (A1TUW0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_972522.1
- ProteinModelPortal:   A1TUW0
- SMR:   A1TUW0
- STRING:   A1TUW0
- GeneID:   4665929
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_4208
- NMPDR:   fig|397945.5.peg.3607
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   A1TUW0
- ProtClustDB:   CLSK2391663
- BioCyc:   AAVE397945:AAVE_4208-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46141; Mature: 46141

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 141-141 BINDING 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYMVGGAVRDRLLGRPVNDRDWVVVGATPDDLAARGFLPVGRDFPVFLHPETREEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKSGRGYRGFVVDTAPDVTLEQDLSRRDLTINAMAVRGEDPAAWELVDPYGGARDL
HHHHHHCCCCCEEEEEECCCCCHHHHCCCCCCEEEEEEEEECCCCCCEEEECCCCCHHHH
QAKLLRHVTDAFREDPVRILRVARFAARFTDFSVAPETMALMREMVDAGEAADLVPERVW
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
QEIARGLMEAAPSRMFEVLRGCGALAVLLPELDRLWGVPQRAEYHPEVDTGVHVMMVLDM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
AARLHAPLAVRFASLVHDLGKGTTPADVLPRHIGHEQRSATLLADVCERLRVPVECRETA
HHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHH
DVVAREHGNIHRSAELGAAALVRLLERCDALRKPARFADVLLACECDARGRLGFEETPYP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCH
QRQRLLGALQAARSVDTAAVAARAQARGAAGPQVGEWIRRARGDAVQEWMAAQPPAAGT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQIYMVGGAVRDRLLGRPVNDRDWVVVGATPDDLAARGFLPVGRDFPVFLHPETREEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCEEECCCCHHHHHH
ARTERKSGRGYRGFVVDTAPDVTLEQDLSRRDLTINAMAVRGEDPAAWELVDPYGGARDL
HHHHHHCCCCCEEEEEECCCCCHHHHCCCCCCEEEEEEEEECCCCCCEEEECCCCCHHHH
QAKLLRHVTDAFREDPVRILRVARFAARFTDFSVAPETMALMREMVDAGEAADLVPERVW
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
QEIARGLMEAAPSRMFEVLRGCGALAVLLPELDRLWGVPQRAEYHPEVDTGVHVMMVLDM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
AARLHAPLAVRFASLVHDLGKGTTPADVLPRHIGHEQRSATLLADVCERLRVPVECRETA
HHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHH
DVVAREHGNIHRSAELGAAALVRLLERCDALRKPARFADVLLACECDARGRLGFEETPYP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCH
QRQRLLGALQAARSVDTAAVAARAQARGAAGPQVGEWIRRARGDAVQEWMAAQPPAAGT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA