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The map label for this gene is slt [H]

Identifier: 120612839

GI number: 120612839

Start: 4657140

End: 4659122

Strand: Reverse

Name: slt [H]

Synonym: Aave_4203

Alternate gene names: 120612839

Gene position: 4659122-4657140 (Counterclockwise)

Preceding gene: 120612841

Following gene: 120612830

Centisome position: 87.04

GC content: 70.05

Gene sequence:

>1983_bases
ATGCAATTGCTCACGATTCTGACATCGCTCCTGGCTGCCGCGGCGTTCGCCTCCGCGTCGGCGCCTGTCCAGGCCCAAAA
CCGGGGCGATGAGGCGCTGTTACAAATGCAGCAGGCCTACCGCAAGGGCGACCGCGCGCGGCTCGCGCAGCTCCTGCCGG
ACGTGCGCGGCCACGTGCTGGAACCCTGGGGCGCCTACTGGGAACTGCGCGCCCGGCTCGACCAGGCCACGCCGCAGGAC
GTGTCGGCCTTCCTGCAGCGCTACGCCGGCACCTACCAGGAAGACCGGCTGCGCAACGACTGGCTGCTGCTCGTCGGCCA
GCGCCGCGACTGGGCGCAGTTCAGCGAATTGCATGCGCTCTACCGCATGAACGACGACCGCGAAGTGCGCTGCTACGCCC
TGCTCGTGGACCAGATCAAGGGCTCCGCGGCCCCCGGCGCGGCCGACGAGGTATTGCGCAACTGGTATGGGTTGCGCGAG
GCGGACGACGGCTGCACGCACGCCGCGGCCGAGTTCTTCTCCGACAAGCGCATCGCTGCGCTGGACGTGTGGCGCAAGGC
CCGGCTGGCGGCCGAGGCCAATCGTGCGCGCGCCACGCGCAATGCGGTCGGGATCGTCGCTCCCGAGGCGCTGCCGCTGC
TGCGCGACGTGTTCGACGCGCCCGCCAAGTTCCTCGCGAGCCGTGCCACGGCACCGGGCCGCATGCGGCAGGAACTGGTG
GTGCTGTCCCTCATCAAGATGGCGATGTCGGACCCCGGCACCGCGGCCCGCCTGCTGGATTCCAAATGGGGTCCGCAGCT
CAGCCCGGAAGAGCGCAACTGGGTGTGGGGCGTGATCGGCAAGCAGTCCGCCGTGGCGCTGTCGCCGGACGCGCTGGGCT
ATTTCGGCAATGTCTCCAGGGATGCCGACCTGACCGACGACATGCTCGGCTGGAAGGTGCGCGCAGCCCTGCGCGCGGGC
CAGTGGAAGCAGGTGGCCCGCGCCATCGACGCGATGAGCCCGGCGGGGCGGCAGGATCCCGCATGGACCTACTGGCGTGC
GCGGGCCTGGCTGTCCAACCGCCCGGGCGACGAAGACCGGGCGAAGGCGCGCGAACTGCTCGAACGCATCGCGGGCCCGG
GCGGCTTCTACGAGCAGCTCGCACTGGAGGAACTCGGCCAGCGCGTGGTCCCGCCCGCGCCGCCGCTGCCGCCCACGCCC
GAGGAAAAGGCCGCCGCGCGCGCGAACCCGGGCTTCGTGCGCAGCCTCTACGCCATCGGCCTGGGGCTGCGGCACGAAGG
CGTGCGCGAATGGAACTACACCACCAACCTGCACTTGCCGGGCGGCATGGGCGACCGGGAACTGCTCGCCGCCGCCGATC
TCGCCTGCCAGCGCGAGATCTGGGACCGCTGCATCAACACCAGCGAACGCACCAAGTCGTTTGCCGACGTGGGCCAGCGC
TTCCCCATGCCATTCCGCAGTGCGGTCGTGGCGCGCGCTCAGTCCATCGGACTGGACCCGGCTTACGTCTATGGTCTCAT
CCGCCAGGAAAGCCGCTTCATCATGGATGCCCGCTCGGGCGTCGGCGCGTCGGGCCTCATGCAGGTCATGCCGGCCACCG
CGCGCTGGACGGCCCGCAAGATCGGCATGGCGGATTTCACGCCCGAGCAGATCAACGACCGCGACACCAACATCGCCATC
GGCACGGCCTACCTCAAGCTCGCTCTCGACGACTTCGACGGCTCCATGCCCATGGCTGCGGCCGCCTACAACGCCGGTCC
CGGCCGGCCGCGCAACTGGCGCAACGGTCCTGTGCTGGAGGCCGCGATCTGGGCCGAGAACATCCCGTTCTCGGAAACAC
GCGATTACGTCAAGAAGGTGCTCTCCAACACCACGATGTATGCCGCGATCCTGACGGGCCAGCCACAGTCGCTCAAGGGC
CGGCTCGGCAACGTGGGCCCGCGCGACGCGGCCACGCCGGACCCGAGCCGCGAACTGCCCTGA

Upstream 100 bases:

>100_bases
CGTTGCAGAAGATCGGCCCGCTGTAGGCGGTCCGGCAGGTTCAGGCGTTCCTCGATCAATGCGCGTCGCAGGGCTGTTTT
GTCCATCACATAATTCCCCA

Downstream 100 bases:

>100_bases
GAACGGACGGGAGCGCCACGCGCGCTCCGTCCAGCGGCAACGCCGGCCTGGCCCTGGCCCGCTCCCTTCAGGCGTTGTGC
AGCGTCTCCCCTTCCAGCCG

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MQLLTILTSLLAAAAFASASAPVQAQNRGDEALLQMQQAYRKGDRARLAQLLPDVRGHVLEPWGAYWELRARLDQATPQD
VSAFLQRYAGTYQEDRLRNDWLLLVGQRRDWAQFSELHALYRMNDDREVRCYALLVDQIKGSAAPGAADEVLRNWYGLRE
ADDGCTHAAAEFFSDKRIAALDVWRKARLAAEANRARATRNAVGIVAPEALPLLRDVFDAPAKFLASRATAPGRMRQELV
VLSLIKMAMSDPGTAARLLDSKWGPQLSPEERNWVWGVIGKQSAVALSPDALGYFGNVSRDADLTDDMLGWKVRAALRAG
QWKQVARAIDAMSPAGRQDPAWTYWRARAWLSNRPGDEDRAKARELLERIAGPGGFYEQLALEELGQRVVPPAPPLPPTP
EEKAAARANPGFVRSLYAIGLGLRHEGVREWNYTTNLHLPGGMGDRELLAAADLACQREIWDRCINTSERTKSFADVGQR
FPMPFRSAVVARAQSIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGMADFTPEQINDRDTNIAI
GTAYLKLALDDFDGSMPMAAAAYNAGPGRPRNWRNGPVLEAAIWAENIPFSETRDYVKKVLSNTTMYAAILTGQPQSLKG
RLGNVGPRDAATPDPSRELP

Sequences:

>Translated_660_residues
MQLLTILTSLLAAAAFASASAPVQAQNRGDEALLQMQQAYRKGDRARLAQLLPDVRGHVLEPWGAYWELRARLDQATPQD
VSAFLQRYAGTYQEDRLRNDWLLLVGQRRDWAQFSELHALYRMNDDREVRCYALLVDQIKGSAAPGAADEVLRNWYGLRE
ADDGCTHAAAEFFSDKRIAALDVWRKARLAAEANRARATRNAVGIVAPEALPLLRDVFDAPAKFLASRATAPGRMRQELV
VLSLIKMAMSDPGTAARLLDSKWGPQLSPEERNWVWGVIGKQSAVALSPDALGYFGNVSRDADLTDDMLGWKVRAALRAG
QWKQVARAIDAMSPAGRQDPAWTYWRARAWLSNRPGDEDRAKARELLERIAGPGGFYEQLALEELGQRVVPPAPPLPPTP
EEKAAARANPGFVRSLYAIGLGLRHEGVREWNYTTNLHLPGGMGDRELLAAADLACQREIWDRCINTSERTKSFADVGQR
FPMPFRSAVVARAQSIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGMADFTPEQINDRDTNIAI
GTAYLKLALDDFDGSMPMAAAAYNAGPGRPRNWRNGPVLEAAIWAENIPFSETRDYVKKVLSNTTMYAAILTGQPQSLKG
RLGNVGPRDAATPDPSRELP
>Mature_660_residues
MQLLTILTSLLAAAAFASASAPVQAQNRGDEALLQMQQAYRKGDRARLAQLLPDVRGHVLEPWGAYWELRARLDQATPQD
VSAFLQRYAGTYQEDRLRNDWLLLVGQRRDWAQFSELHALYRMNDDREVRCYALLVDQIKGSAAPGAADEVLRNWYGLRE
ADDGCTHAAAEFFSDKRIAALDVWRKARLAAEANRARATRNAVGIVAPEALPLLRDVFDAPAKFLASRATAPGRMRQELV
VLSLIKMAMSDPGTAARLLDSKWGPQLSPEERNWVWGVIGKQSAVALSPDALGYFGNVSRDADLTDDMLGWKVRAALRAG
QWKQVARAIDAMSPAGRQDPAWTYWRARAWLSNRPGDEDRAKARELLERIAGPGGFYEQLALEELGQRVVPPAPPLPPTP
EEKAAARANPGFVRSLYAIGLGLRHEGVREWNYTTNLHLPGGMGDRELLAAADLACQREIWDRCINTSERTKSFADVGQR
FPMPFRSAVVARAQSIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGMADFTPEQINDRDTNIAI
GTAYLKLALDDFDGSMPMAAAAYNAGPGRPRNWRNGPVLEAAIWAENIPFSETRDYVKKVLSNTTMYAAILTGQPQSLKG
RLGNVGPRDAATPDPSRELP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=327, Percent_Identity=29.3577981651376, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72887; Mature: 72887

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLTILTSLLAAAAFASASAPVQAQNRGDEALLQMQQAYRKGDRARLAQLLPDVRGHVL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
EPWGAYWELRARLDQATPQDVSAFLQRYAGTYQEDRLRNDWLLLVGQRRDWAQFSELHAL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHH
YRMNDDREVRCYALLVDQIKGSAAPGAADEVLRNWYGLREADDGCTHAAAEFFSDKRIAA
HHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHH
LDVWRKARLAAEANRARATRNAVGIVAPEALPLLRDVFDAPAKFLASRATAPGRMRQELV
HHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VLSLIKMAMSDPGTAARLLDSKWGPQLSPEERNWVWGVIGKQSAVALSPDALGYFGNVSR
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCHHHHHCCCCCC
DADLTDDMLGWKVRAALRAGQWKQVARAIDAMSPAGRQDPAWTYWRARAWLSNRPGDEDR
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHH
AKARELLERIAGPGGFYEQLALEELGQRVVPPAPPLPPTPEEKAAARANPGFVRSLYAIG
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
LGLRHEGVREWNYTTNLHLPGGMGDRELLAAADLACQREIWDRCINTSERTKSFADVGQR
CCHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FPMPFRSAVVARAQSIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHH
IGMADFTPEQINDRDTNIAIGTAYLKLALDDFDGSMPMAAAAYNAGPGRPRNWRNGPVLE
CCCCCCCHHHCCCCCCCEEEEHHEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCEE
AAIWAENIPFSETRDYVKKVLSNTTMYAAILTGQPQSLKGRLGNVGPRDAATPDPSRELP
HHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLLTILTSLLAAAAFASASAPVQAQNRGDEALLQMQQAYRKGDRARLAQLLPDVRGHVL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
EPWGAYWELRARLDQATPQDVSAFLQRYAGTYQEDRLRNDWLLLVGQRRDWAQFSELHAL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHH
YRMNDDREVRCYALLVDQIKGSAAPGAADEVLRNWYGLREADDGCTHAAAEFFSDKRIAA
HHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHH
LDVWRKARLAAEANRARATRNAVGIVAPEALPLLRDVFDAPAKFLASRATAPGRMRQELV
HHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VLSLIKMAMSDPGTAARLLDSKWGPQLSPEERNWVWGVIGKQSAVALSPDALGYFGNVSR
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCHHHHHCCCCCC
DADLTDDMLGWKVRAALRAGQWKQVARAIDAMSPAGRQDPAWTYWRARAWLSNRPGDEDR
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHH
AKARELLERIAGPGGFYEQLALEELGQRVVPPAPPLPPTPEEKAAARANPGFVRSLYAIG
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
LGLRHEGVREWNYTTNLHLPGGMGDRELLAAADLACQREIWDRCINTSERTKSFADVGQR
CCHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FPMPFRSAVVARAQSIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHH
IGMADFTPEQINDRDTNIAIGTAYLKLALDDFDGSMPMAAAAYNAGPGRPRNWRNGPVLE
CCCCCCCHHHCCCCCCCEEEEHHEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCEE
AAIWAENIPFSETRDYVKKVLSNTTMYAAILTGQPQSLKGRLGNVGPRDAATPDPSRELP
HHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]