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The map label for this gene is purR [H]

Identifier: 120612835

GI number: 120612835

Start: 4653459

End: 4654520

Strand: Direct

Name: purR [H]

Synonym: Aave_4199

Alternate gene names: 120612835

Gene position: 4653459-4654520 (Clockwise)

Preceding gene: 120612834

Following gene: 120612836

Centisome position: 86.94

GC content: 71.0

Gene sequence:

>1062_bases
ATGGCGACCATCAAGGACGTCGCGCGCGAGGCCGGGGTGTCGGTGACCACCGTTTCGCACGTGCTCAACGGCACGCGGCA
CGTGAGCGACTACGGGCGCGCGCGCGTGGAGGCCGCCATCCGGCAACTGGGTTACGTGCCCAGCGCGATCGCGCGCAGCC
TCAAGAGCAACCACACGCGCACGCTGGGGATGCTGATCCCCAACAGTTCCAACCCCTACTTCGCCGAGGTCGTGCACAGC
GTGGAGAACCGCTGCTTCGGCGCGGGCTACAACCTCATCCTCTGCAATACCCACGACGAGGCCCAGCGGCAAAGCTCGTA
CCTCCAGGTGCTGGCGGAGCGGCGCATCGACGGGCTGATCGTGGTGACCACCGGGCAGGACGCGGCGCTCGCGCGCCAGC
TCGAGGGGCTGGGCGTGCCCACCGTGCTGGTGGACCGCGAGATCGAGGTCTCCCGGCAGCCCTGCGACCTGGTGGAGACC
GCGCACCGCGAAGGCGGCCGGCTGGCTACGCGCCACCTGCTCTGGCTGGGCCACCGTCGCATCGCCTGCATCGGCGGTCC
CCCGGACCTTGCTCCCAGCATGCAGCGCATCGAGGGCTGGCGCGACGCGCTGGCCGAAGCGGGCGTGGACGATGGCGGCC
TGCTGTGGCACGGCGATTTCACCAGCCAGAGCGGCTACGAGGCCATGCAAGCGGTGCTGGCCTCGCCGCGCCCGCCCACG
GCGGTGTTCATGGGCAACGACCTGATGGCCATCGGTGCGCTCTGCGCGGCCCACGAACGCGGGCTGCGCGTGCCCGAGGC
CATGTCCATCGTGGGCTTCGACGATATCGCGCTCGCGGCCTTCACGAGCCCGCCCCTCACCACCGTGGCCCAGCCCAAGC
GGCAGATCGGCGTGGCTGCCGTGGACATGCTGCTCGAACGCATCGACGGCGCGCGGCGCGAACCGTGCCAGTTGCTGCTG
CAACCGGAGCTGCGGCTGCGCGCCTCGACCGCGGCATTGCCCCAGGGCCCCGTTTCCACCGCCGCGCCGCACGGCCGTTT
TCCTTCCCGCCATCCCGAATGA

Upstream 100 bases:

>100_bases
CGAGCAAGCGCATCATCACCGGCTGCGTGATCGTGGCGGCCGTCATCGTCGACACGCTGCGGCAGCGCCGCGCCGCGGTC
TGACGCCGGCACGCGAAGAC

Downstream 100 bases:

>100_bases
CCGTCTCTTCCCGCTCCGCTCAGGAGAACCATTCCCGCTCCGCTATGCCGCGCATCGCCGTCGTGGGCAGTCTCAACATG
GACATCGTCCTGCGCGTTCC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MATIKDVAREAGVSVTTVSHVLNGTRHVSDYGRARVEAAIRQLGYVPSAIARSLKSNHTRTLGMLIPNSSNPYFAEVVHS
VENRCFGAGYNLILCNTHDEAQRQSSYLQVLAERRIDGLIVVTTGQDAALARQLEGLGVPTVLVDREIEVSRQPCDLVET
AHREGGRLATRHLLWLGHRRIACIGGPPDLAPSMQRIEGWRDALAEAGVDDGGLLWHGDFTSQSGYEAMQAVLASPRPPT
AVFMGNDLMAIGALCAAHERGLRVPEAMSIVGFDDIALAAFTSPPLTTVAQPKRQIGVAAVDMLLERIDGARREPCQLLL
QPELRLRASTAALPQGPVSTAAPHGRFPSRHPE

Sequences:

>Translated_353_residues
MATIKDVAREAGVSVTTVSHVLNGTRHVSDYGRARVEAAIRQLGYVPSAIARSLKSNHTRTLGMLIPNSSNPYFAEVVHS
VENRCFGAGYNLILCNTHDEAQRQSSYLQVLAERRIDGLIVVTTGQDAALARQLEGLGVPTVLVDREIEVSRQPCDLVET
AHREGGRLATRHLLWLGHRRIACIGGPPDLAPSMQRIEGWRDALAEAGVDDGGLLWHGDFTSQSGYEAMQAVLASPRPPT
AVFMGNDLMAIGALCAAHERGLRVPEAMSIVGFDDIALAAFTSPPLTTVAQPKRQIGVAAVDMLLERIDGARREPCQLLL
QPELRLRASTAALPQGPVSTAAPHGRFPSRHPE
>Mature_352_residues
ATIKDVAREAGVSVTTVSHVLNGTRHVSDYGRARVEAAIRQLGYVPSAIARSLKSNHTRTLGMLIPNSSNPYFAEVVHSV
ENRCFGAGYNLILCNTHDEAQRQSSYLQVLAERRIDGLIVVTTGQDAALARQLEGLGVPTVLVDREIEVSRQPCDLVETA
HREGGRLATRHLLWLGHRRIACIGGPPDLAPSMQRIEGWRDALAEAGVDDGGLLWHGDFTSQSGYEAMQAVLASPRPPTA
VFMGNDLMAIGALCAAHERGLRVPEAMSIVGFDDIALAAFTSPPLTTVAQPKRQIGVAAVDMLLERIDGARREPCQLLLQ
PELRLRASTAALPQGPVSTAAPHGRFPSRHPE

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=45.3172205438066, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=44.9404761904762, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=36.5781710914454, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1789202, Length=297, Percent_Identity=35.3535353535354, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=31.6614420062696, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786540, Length=320, Percent_Identity=32.1875, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=33.7313432835821, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=27.9365079365079, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=29.0625, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789456, Length=346, Percent_Identity=29.7687861271676, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787906, Length=322, Percent_Identity=27.3291925465839, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786268, Length=314, Percent_Identity=24.5222929936306, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790715, Length=317, Percent_Identity=27.1293375394322, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=26.5625, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38084; Mature: 37953

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAREAGVSVTTVSHVLNGTRHVSDYGRARVEAAIRQLGYVPSAIARSLKSNHTR
CCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
TLGMLIPNSSNPYFAEVVHSVENRCFGAGYNLILCNTHDEAQRQSSYLQVLAERRIDGLI
EEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEE
VVTTGQDAALARQLEGLGVPTVLVDREIEVSRQPCDLVETAHREGGRLATRHLLWLGHRR
EEECCCHHHHHHHHHCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCE
IACIGGPPDLAPSMQRIEGWRDALAEAGVDDGGLLWHGDFTSQSGYEAMQAVLASPRPPT
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCE
AVFMGNDLMAIGALCAAHERGLRVPEAMSIVGFDDIALAAFTSPPLTTVAQPKRQIGVAA
EEEECCCHHHHHHHHHHHHCCCCCCHHHEEECCCHHHHEEECCCCCCHHHCHHHHHHHHH
VDMLLERIDGARREPCQLLLQPELRLRASTAALPQGPVSTAAPHGRFPSRHPE
HHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAREAGVSVTTVSHVLNGTRHVSDYGRARVEAAIRQLGYVPSAIARSLKSNHTR
CCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
TLGMLIPNSSNPYFAEVVHSVENRCFGAGYNLILCNTHDEAQRQSSYLQVLAERRIDGLI
EEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEE
VVTTGQDAALARQLEGLGVPTVLVDREIEVSRQPCDLVETAHREGGRLATRHLLWLGHRR
EEECCCHHHHHHHHHCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCE
IACIGGPPDLAPSMQRIEGWRDALAEAGVDDGGLLWHGDFTSQSGYEAMQAVLASPRPPT
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCE
AVFMGNDLMAIGALCAAHERGLRVPEAMSIVGFDDIALAAFTSPPLTTVAQPKRQIGVAA
EEEECCCHHHHHHHHHHHHCCCCCCHHHEEECCCHHHHEEECCCCCCHHHCHHHHHHHHH
VDMLLERIDGARREPCQLLLQPELRLRASTAALPQGPVSTAAPHGRFPSRHPE
HHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA