Click here to switch to the map view.

The map label for this gene is ansB [H]

Identifier: 120612819

GI number: 120612819

Start: 4634052

End: 4635149

Strand: Reverse

Name: ansB [H]

Synonym: Aave_4182

Alternate gene names: 120612819

Gene position: 4635149-4634052 (Counterclockwise)

Preceding gene: 120612821

Following gene: 120612818

Centisome position: 86.59

GC content: 67.49

Gene sequence:

>1098_bases
ATGTTCCCGCAAATGAATATCAAGCGCCTGCTGGCCGGCGCCGCCTTCGCTGCCTGGGGCCTCGTGGCCGGTGCGCAGAC
GCAGTCCGCCGCCCCCACGCAGCCGGCGGCCAAGCCCAACGTCGTGGTGCTGGCCACCGGCGGCACCATCGCAGGCGCAG
GCGCCACGGTCGCCAACAGCGCTTCCTATGCCGCGGCCAAGGTGCCGGTGGACAAGCTGCTGGCAGGGCTGCCGGAGCTG
TCCAACGTGGCGGCCGTGAAGGGCGAGCAGGTCTTCCAGATCGCTTCCGAGAGCTTCACCAACGACCAGTTGCTGCAACT
GGGCAAGCGTGTCTCCGCGCTGTCCAAGCAATCCGACGTGGACGGCATCGTCATCACGCACGGCACCGACACGCTGGAAG
AGACCGCCTACTTCCTGAACCTGGTCGTCCACACCAACAAGCCCATCGTCGTCGTCGGTTCCATGCGCCCGGGCACCGCC
CTGTCCGCGGACGGCGCGCTGAACCTGCTGGAGGCCGTCTCGGTGGCCGCCAGCAAGGAGTCTGCCGGCAAGGGCGTGCT
GGTGACCATGAGCGACGAGATCCAGAGCGGCCGCGACGTGACCAAGGGCGTCAACATCAAGACCCAGGCGTTCCGCAGCC
AGTGGGGCCCGCTGGGCATGGTGGTGGAAGGCAAGACCTACTGGTTCCGCGCCCCCGTCAAGCGCCACACGATGCAGTCG
GAGTTCAACATCGACGACATCACCACCCTGGCGCCCGTGGACATCGTCTATGGATACGGCAACGTGCCGCGCTCGACCAT
CGATGCCGTGGGCAATTCGGGCGTGAAAGCCCTGATCCACGCCGGCATGGGCAACGGTTCCGTGGCCGACCGCGTGGTGC
CTGCCCTGCAGGAGCTGCGCGGCAAGGGCGTGCAGATCGTGCGCTCCTCGCGCGTGGCCGACGGGTTCGTGCTGCGCAAC
GCCGAGCAGCCCGACGACAAGTACGACTGGGTCGTCGCGCACGACCTGAACCCCCAGAAGGCCCGCATCCTGGCGGCCGT
CGCCCTGACCAAGCCCCAGACCAGCCAGGAACTGCAGCGCATCTTCTGGCAATACTGA

Upstream 100 bases:

>100_bases
CCCGGCCCTGGATTATTCCCCACTGCCGTGTTGTGGCCTTCCTACCATACTGCACCCGTTTCACGGGCTTGTAACAATTA
ATCAAGGAGATGAACGAGTC

Downstream 100 bases:

>100_bases
GCGGCTCGGCCGCACGGCCCGGCGGCACGCCTGCCGCCGGGCCGGATCTCCCGAAGGGCCGCCGTGCGCGGCCCTTTTTA
TTGCGCGGCGTCAAGCGGGT

Product: L-asparaginases

Products: NA

Alternate protein names: L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MFPQMNIKRLLAGAAFAAWGLVAGAQTQSAAPTQPAAKPNVVVLATGGTIAGAGATVANSASYAAAKVPVDKLLAGLPEL
SNVAAVKGEQVFQIASESFTNDQLLQLGKRVSALSKQSDVDGIVITHGTDTLEETAYFLNLVVHTNKPIVVVGSMRPGTA
LSADGALNLLEAVSVAASKESAGKGVLVTMSDEIQSGRDVTKGVNIKTQAFRSQWGPLGMVVEGKTYWFRAPVKRHTMQS
EFNIDDITTLAPVDIVYGYGNVPRSTIDAVGNSGVKALIHAGMGNGSVADRVVPALQELRGKGVQIVRSSRVADGFVLRN
AEQPDDKYDWVVAHDLNPQKARILAAVALTKPQTSQELQRIFWQY

Sequences:

>Translated_365_residues
MFPQMNIKRLLAGAAFAAWGLVAGAQTQSAAPTQPAAKPNVVVLATGGTIAGAGATVANSASYAAAKVPVDKLLAGLPEL
SNVAAVKGEQVFQIASESFTNDQLLQLGKRVSALSKQSDVDGIVITHGTDTLEETAYFLNLVVHTNKPIVVVGSMRPGTA
LSADGALNLLEAVSVAASKESAGKGVLVTMSDEIQSGRDVTKGVNIKTQAFRSQWGPLGMVVEGKTYWFRAPVKRHTMQS
EFNIDDITTLAPVDIVYGYGNVPRSTIDAVGNSGVKALIHAGMGNGSVADRVVPALQELRGKGVQIVRSSRVADGFVLRN
AEQPDDKYDWVVAHDLNPQKARILAAVALTKPQTSQELQRIFWQY
>Mature_365_residues
MFPQMNIKRLLAGAAFAAWGLVAGAQTQSAAPTQPAAKPNVVVLATGGTIAGAGATVANSASYAAAKVPVDKLLAGLPEL
SNVAAVKGEQVFQIASESFTNDQLLQLGKRVSALSKQSDVDGIVITHGTDTLEETAYFLNLVVHTNKPIVVVGSMRPGTA
LSADGALNLLEAVSVAASKESAGKGVLVTMSDEIQSGRDVTKGVNIKTQAFRSQWGPLGMVVEGKTYWFRAPVKRHTMQS
EFNIDDITTLAPVDIVYGYGNVPRSTIDAVGNSGVKALIHAGMGNGSVADRVVPALQELRGKGVQIVRSSRVADGFVLRN
AEQPDDKYDWVVAHDLNPQKARILAAVALTKPQTSQELQRIFWQY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=330, Percent_Identity=44.2424242424242, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788065, Length=286, Percent_Identity=26.5734265734266, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323189, Length=331, Percent_Identity=36.5558912386707, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6323187, Length=331, Percent_Identity=36.5558912386707, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6323186, Length=331, Percent_Identity=36.5558912386707, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6323184, Length=331, Percent_Identity=36.5558912386707, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6320527, Length=339, Percent_Identity=34.2182890855457, Blast_Score=172, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.38 [H]

Molecular weight: Translated: 38572; Mature: 38572

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPQMNIKRLLAGAAFAAWGLVAGAQTQSAAPTQPAAKPNVVVLATGGTIAGAGATVANS
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCHHCCC
ASYAAAKVPVDKLLAGLPELSNVAAVKGEQVFQIASESFTNDQLLQLGKRVSALSKQSDV
CCHHHHCCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DGIVITHGTDTLEETAYFLNLVVHTNKPIVVVGSMRPGTALSADGALNLLEAVSVAASKE
CEEEEECCCCHHHHHHHEEEEEEECCCCEEEEECCCCCCEECCCCHHHHHHHHHHHHCCC
SAGKGVLVTMSDEIQSGRDVTKGVNIKTQAFRSQWGPLGMVVEGKTYWFRAPVKRHTMQS
CCCCEEEEEECHHHCCCCCHHCCCCEEHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHC
EFNIDDITTLAPVDIVYGYGNVPRSTIDAVGNSGVKALIHAGMGNGSVADRVVPALQELR
CCCCCHHHHHCCEEEEECCCCCCHHHHHHHCCCCCEEHEECCCCCCHHHHHHHHHHHHHC
GKGVQIVRSSRVADGFVLRNAEQPDDKYDWVVAHDLNPQKARILAAVALTKPQTSQELQR
CCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHEEEEEEECCCCCHHHHHH
IFWQY
HHHCC
>Mature Secondary Structure
MFPQMNIKRLLAGAAFAAWGLVAGAQTQSAAPTQPAAKPNVVVLATGGTIAGAGATVANS
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCHHCCC
ASYAAAKVPVDKLLAGLPELSNVAAVKGEQVFQIASESFTNDQLLQLGKRVSALSKQSDV
CCHHHHCCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DGIVITHGTDTLEETAYFLNLVVHTNKPIVVVGSMRPGTALSADGALNLLEAVSVAASKE
CEEEEECCCCHHHHHHHEEEEEEECCCCEEEEECCCCCCEECCCCHHHHHHHHHHHHCCC
SAGKGVLVTMSDEIQSGRDVTKGVNIKTQAFRSQWGPLGMVVEGKTYWFRAPVKRHTMQS
CCCCEEEEEECHHHCCCCCHHCCCCEEHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHC
EFNIDDITTLAPVDIVYGYGNVPRSTIDAVGNSGVKALIHAGMGNGSVADRVVPALQELR
CCCCCHHHHHCCEEEEECCCCCCHHHHHHHCCCCCEEHEECCCCCCHHHHHHHHHHHHHC
GKGVQIVRSSRVADGFVLRNAEQPDDKYDWVVAHDLNPQKARILAAVALTKPQTSQELQR
CCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHEEEEEEECCCCCHHHHHH
IFWQY
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]