Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 120612695

GI number: 120612695

Start: 4508225

End: 4509073

Strand: Reverse

Name: metF [H]

Synonym: Aave_4058

Alternate gene names: 120612695

Gene position: 4509073-4508225 (Counterclockwise)

Preceding gene: 120612696

Following gene: 120612694

Centisome position: 84.24

GC content: 68.79

Gene sequence:

>849_bases
ATGAGCCTTTCCACGCACGAGGCGGCCCTTCCGGTCAGCTTCGAATTCTTCCCTCCCAAGACGCCCGAGGGCGCGGACAA
GCTGCGCACCGTGCGCCAGCAGCTCTACGCCCACCGTCCCGAGTTCTGCTCCGTCACCTACGGCGCGGGCGGCTCCACCC
AGGAAGGCACCTTCGGCACGGTGCGCGAGATCCTGTCCGAAGGCGTGCAGGCGGCCTCGCATTTTTCGTGCATCGGCGCC
ACCCGCGAGGGCGTGCGCACGCAGCTCGCCCAGCTCAAGGCCATGGGCGTGTCCCGGCTGGTGGCGCTGCGCGGCGACCT
GCCCAGCGGCTATGGCGCCGGCGGGGAGTTCCAGTACGCCAGCGACCTGGTGGCTTTCATCCGCGCCGAGGCGGGCGATG
ATTTCCATATCGAGGTGGCCGCCTATCCGGAAGTCCATCCGCAGGCCCGCTCCCCCGAGGCCGACCTGCAGGCGTTCGCC
ACCAAGGTACGCGCCGGGGCGCATTCGGCCATCACGCAGTATTTCTTCAATCCCGACGCCTACTGGCGCTTCGTGGACGA
CGTGCGCGCCCTGGGGCTCGACGTGCCGGTGATACCGGGCATCATGCCGATCATGGGATCGACCCAGCTCATGCGTTTTT
CCGACGCTTGCGGCGCGGAAATCCCCCGCTGGATCCGGTTGCGGCTGCTGGGCTTCGGCGACGACTCGGCCAGCATCCGC
GCTTTCGGGCTCGACGTGGTGACTGCGCTGTGCGAACGCCTGCGCGCCGGCGGGGCGCCCTCGCTGCACTTCTACACCAT
GAACCAGAGCGCCGCGACGCTGGAGATCTGCCGGCGCCTGGGGCTTTGA

Upstream 100 bases:

>100_bases
GCTGCGCCGCGGCGGGCCTGGACGTGGCCGGGTGGCACGACAGCCCGATCGACGGCGGCGACGGCAACCGCGAATTCTTC
ATCCACGCAAGGAGGAAGGC

Downstream 100 bases:

>100_bases
CCCGCGACGGAGGCCGGCGGCGGACTCGGCCGTGTTGGGTAATATGGCGCCTCCCCGCGCGACCCTGACATGACGCCCGT
TCTCGCTTCTTCCCTGATGG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSLSTHEAALPVSFEFFPPKTPEGADKLRTVRQQLYAHRPEFCSVTYGAGGSTQEGTFGTVREILSEGVQAASHFSCIGA
TREGVRTQLAQLKAMGVSRLVALRGDLPSGYGAGGEFQYASDLVAFIRAEAGDDFHIEVAAYPEVHPQARSPEADLQAFA
TKVRAGAHSAITQYFFNPDAYWRFVDDVRALGLDVPVIPGIMPIMGSTQLMRFSDACGAEIPRWIRLRLLGFGDDSASIR
AFGLDVVTALCERLRAGGAPSLHFYTMNQSAATLEICRRLGL

Sequences:

>Translated_282_residues
MSLSTHEAALPVSFEFFPPKTPEGADKLRTVRQQLYAHRPEFCSVTYGAGGSTQEGTFGTVREILSEGVQAASHFSCIGA
TREGVRTQLAQLKAMGVSRLVALRGDLPSGYGAGGEFQYASDLVAFIRAEAGDDFHIEVAAYPEVHPQARSPEADLQAFA
TKVRAGAHSAITQYFFNPDAYWRFVDDVRALGLDVPVIPGIMPIMGSTQLMRFSDACGAEIPRWIRLRLLGFGDDSASIR
AFGLDVVTALCERLRAGGAPSLHFYTMNQSAATLEICRRLGL
>Mature_281_residues
SLSTHEAALPVSFEFFPPKTPEGADKLRTVRQQLYAHRPEFCSVTYGAGGSTQEGTFGTVREILSEGVQAASHFSCIGAT
REGVRTQLAQLKAMGVSRLVALRGDLPSGYGAGGEFQYASDLVAFIRAEAGDDFHIEVAAYPEVHPQARSPEADLQAFAT
KVRAGAHSAITQYFFNPDAYWRFVDDVRALGLDVPVIPGIMPIMGSTQLMRFSDACGAEIPRWIRLRLLGFGDDSASIRA
FGLDVVTALCERLRAGGAPSLHFYTMNQSAATLEICRRLGL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=279, Percent_Identity=38.7096774193548, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1790377, Length=282, Percent_Identity=40.0709219858156, Blast_Score=212, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=35.6115107913669, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=35.6115107913669, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321313, Length=281, Percent_Identity=33.4519572953737, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=31.6326530612245, Blast_Score=133, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30551; Mature: 30420

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSTHEAALPVSFEFFPPKTPEGADKLRTVRQQLYAHRPEFCSVTYGAGGSTQEGTFGT
CCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHH
VREILSEGVQAASHFSCIGATREGVRTQLAQLKAMGVSRLVALRGDLPSGYGAGGEFQYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
SDLVAFIRAEAGDDFHIEVAAYPEVHPQARSPEADLQAFATKVRAGAHSAITQYFFNPDA
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCH
YWRFVDDVRALGLDVPVIPGIMPIMGSTQLMRFSDACGAEIPRWIRLRLLGFGDDSASIR
HHHHHHHHHHHCCCCCHHCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEE
AFGLDVVTALCERLRAGGAPSLHFYTMNQSAATLEICRRLGL
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
SLSTHEAALPVSFEFFPPKTPEGADKLRTVRQQLYAHRPEFCSVTYGAGGSTQEGTFGT
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHH
VREILSEGVQAASHFSCIGATREGVRTQLAQLKAMGVSRLVALRGDLPSGYGAGGEFQYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
SDLVAFIRAEAGDDFHIEVAAYPEVHPQARSPEADLQAFATKVRAGAHSAITQYFFNPDA
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCH
YWRFVDDVRALGLDVPVIPGIMPIMGSTQLMRFSDACGAEIPRWIRLRLLGFGDDSASIR
HHHHHHHHHHHCCCCCHHCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEE
AFGLDVVTALCERLRAGGAPSLHFYTMNQSAATLEICRRLGL
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]