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The map label for this gene is tyrS [H]

Identifier: 120612682

GI number: 120612682

Start: 4495515

End: 4496747

Strand: Reverse

Name: tyrS [H]

Synonym: Aave_4045

Alternate gene names: 120612682

Gene position: 4496747-4495515 (Counterclockwise)

Preceding gene: 120612685

Following gene: 120612680

Centisome position: 84.01

GC content: 65.69

Gene sequence:

>1233_bases
ATGAATCAATCCGCTGTTACAACATTCCCGGTCACCGATGGTGTAGGACGGGCGCTGGAGGTGTCATTGCGCGGAGTCGA
GGAATTGCTGCCCCGGGACGAGTGGACGCGCAAGCTCGCGCGTTCCGAGGCGACGGGGATTCCGCTGCGCATCAAGCTGG
GCCTCGACCCCACGGCGCCGGACATCCACATCGGCCACACGGTGGTGCTCAACAAGATGCGCCAGCTGCAGGACCTGGGG
CACCAGGTGATCTTCCTCATCGGTGATTTCACCACGCTGATCGGCGACCCGTCCGGCCGCAATTCCACGCGTCCGCCGCT
CACGCCGGAACAGATCAAGGTGAATGCGGAGACCTACTACCGCCAGGCGAGCATGGTGCTGGACCCCGCGAAGACCGAGA
TCCGCTACAACAGCGAATGGTGCGAGCCGCTGGGCGCGGCCGGCATGATCCAGCTGGCGGCCAAGTACACCGTGGCCCGC
ATGATGGAGCGCAACGATTTCCACCAGCGGTTCACGGACGGCAGCTCCATCAGCCTGCACGAGTTCCTCTACCCGCTGCT
GCAGGGCTACGACTCCGTGGCGCTCAGGAGCGACCTGGAGCTGGGTGGCACGGACCAGAAGTTCAACCTGCTGATGGGGC
GCCACCTGCAGCAGGAGTACGGCCAGGAGCCGCAGTGCATCCTGACGATGCCGCTGCTGGTGGGGTTGGACGGCGTGGAC
AAGATGTCCAAGTCCAAGGGCAATTACATCGGCATCACCGAAGACGCCAACACCATGTTCGCCAAGGTGCTGTCCATCTC
GGACGACCTGATGTGGGACTGGTACACGCTGCTGTCGTTCAAGTCGCTGGCGGAGATCGCCGCGCTGCGCCAGGAAGTGG
AGGGTGGGCGCAACCCCAAGGACGCCAAGGTGATGCTGGCCCGGGAAATCACCACGCGCTTCCACAGCGCCGCCGCGGCC
GATGCGGCCGAAGCGGATTTCGCCAACCGCAGCAAGGGCGGCGTGCCGGACGAGATCCCCGAGGTGTCGCTGGAGGGCGC
GCCGCTGGGCATCGGCGCCCTGCTCAAGCAGGCGAACCTAGCTCCGTCCACGAGCGAAGCCAACCGCCTGATCGACGGCG
GCGGCGTGCGCGTGGAAGGCAGCGTGGTGAGCGACCGCGGCCTGAAACTGGAGGCGGGTACCTATGTGGTGCAGGTGGGC
AAGCGCAAGTTCGCCCGCGTGACGCTGGGCTGA

Upstream 100 bases:

>100_bases
GGGCCGGAAGGCGGAGCCGGCGGGCGCACTAGAATCCGCCGCTGCAAAGGTGGCAGGAAGTATAGCCACCGACCTACTTG
GCGAACCCCGTAAAACCCTT

Downstream 100 bases:

>100_bases
CACCAGAACCCGGGGCCTGGGCCCGGGTGCCGCCGCTGGTCAGCCCAGGTCGTCGTTCAGGTCCGGATCGGGGACTTCGC
CCACGGCTTCACGGGTGAGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNQSAVTTFPVTDGVGRALEVSLRGVEELLPRDEWTRKLARSEATGIPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLG
HQVIFLIGDFTTLIGDPSGRNSTRPPLTPEQIKVNAETYYRQASMVLDPAKTEIRYNSEWCEPLGAAGMIQLAAKYTVAR
MMERNDFHQRFTDGSSISLHEFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEYGQEPQCILTMPLLVGLDGVD
KMSKSKGNYIGITEDANTMFAKVLSISDDLMWDWYTLLSFKSLAEIAALRQEVEGGRNPKDAKVMLAREITTRFHSAAAA
DAAEADFANRSKGGVPDEIPEVSLEGAPLGIGALLKQANLAPSTSEANRLIDGGGVRVEGSVVSDRGLKLEAGTYVVQVG
KRKFARVTLG

Sequences:

>Translated_410_residues
MNQSAVTTFPVTDGVGRALEVSLRGVEELLPRDEWTRKLARSEATGIPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLG
HQVIFLIGDFTTLIGDPSGRNSTRPPLTPEQIKVNAETYYRQASMVLDPAKTEIRYNSEWCEPLGAAGMIQLAAKYTVAR
MMERNDFHQRFTDGSSISLHEFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEYGQEPQCILTMPLLVGLDGVD
KMSKSKGNYIGITEDANTMFAKVLSISDDLMWDWYTLLSFKSLAEIAALRQEVEGGRNPKDAKVMLAREITTRFHSAAAA
DAAEADFANRSKGGVPDEIPEVSLEGAPLGIGALLKQANLAPSTSEANRLIDGGGVRVEGSVVSDRGLKLEAGTYVVQVG
KRKFARVTLG
>Mature_410_residues
MNQSAVTTFPVTDGVGRALEVSLRGVEELLPRDEWTRKLARSEATGIPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLG
HQVIFLIGDFTTLIGDPSGRNSTRPPLTPEQIKVNAETYYRQASMVLDPAKTEIRYNSEWCEPLGAAGMIQLAAKYTVAR
MMERNDFHQRFTDGSSISLHEFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEYGQEPQCILTMPLLVGLDGVD
KMSKSKGNYIGITEDANTMFAKVLSISDDLMWDWYTLLSFKSLAEIAALRQEVEGGRNPKDAKVMLAREITTRFHSAAAA
DAAEADFANRSKGGVPDEIPEVSLEGAPLGIGALLKQANLAPSTSEANRLIDGGGVRVEGSVVSDRGLKLEAGTYVVQVG
KRKFARVTLG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=400, Percent_Identity=25.5, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1787925, Length=400, Percent_Identity=27.5, Blast_Score=124, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=291, Percent_Identity=26.8041237113402, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=306, Percent_Identity=23.5294117647059, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130383, Length=400, Percent_Identity=27, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44901; Mature: 44901

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSAVTTFPVTDGVGRALEVSLRGVEELLPRDEWTRKLARSEATGIPLRIKLGLDPTAP
CCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
DIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTTLIGDPSGRNSTRPPLTPEQIKVNAETYY
CEECCHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHHEEECHHHHH
RQASMVLDPAKTEIRYNSEWCEPLGAAGMIQLAAKYTVARMMERNDFHQRFTDGSSISLH
HHHHHEECCCHHCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEYGQEPQCILTMPLLVGLDGVD
HHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCHH
KMSKSKGNYIGITEDANTMFAKVLSISDDLMWDWYTLLSFKSLAEIAALRQEVEGGRNPK
HHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAKVMLAREITTRFHSAAAADAAEADFANRSKGGVPDEIPEVSLEGAPLGIGALLKQANL
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
APSTSEANRLIDGGGVRVEGSVVSDRGLKLEAGTYVVQVGKRKFARVTLG
CCCCHHHHHEECCCCEEEECEEECCCCCEEECCCEEEEECCCEEEEEECC
>Mature Secondary Structure
MNQSAVTTFPVTDGVGRALEVSLRGVEELLPRDEWTRKLARSEATGIPLRIKLGLDPTAP
CCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
DIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTTLIGDPSGRNSTRPPLTPEQIKVNAETYY
CEECCHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHHEEECHHHHH
RQASMVLDPAKTEIRYNSEWCEPLGAAGMIQLAAKYTVARMMERNDFHQRFTDGSSISLH
HHHHHEECCCHHCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEYGQEPQCILTMPLLVGLDGVD
HHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCHH
KMSKSKGNYIGITEDANTMFAKVLSISDDLMWDWYTLLSFKSLAEIAALRQEVEGGRNPK
HHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAKVMLAREITTRFHSAAAADAAEADFANRSKGGVPDEIPEVSLEGAPLGIGALLKQANL
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
APSTSEANRLIDGGGVRVEGSVVSDRGLKLEAGTYVVQVGKRKFARVTLG
CCCCHHHHHEECCCCEEEECEEECCCCCEEECCCEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA