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The map label for this gene is gcvA [H]

Identifier: 120612681

GI number: 120612681

Start: 4494483

End: 4495475

Strand: Direct

Name: gcvA [H]

Synonym: Aave_4044

Alternate gene names: 120612681

Gene position: 4494483-4495475 (Clockwise)

Preceding gene: 120612678

Following gene: 120612683

Centisome position: 83.97

GC content: 70.39

Gene sequence:

>993_bases
ATGACAATTCCCGCCCCTGTCGCTCACCTGCGCACGCGGCCCGTGGCGGTCGGCCACTGGCGCGCCTTCCTGGCCGTGGC
CCGGCACCTGAACTTCCGCGCCGCCGCCGAGGAACTGTCCCTCACGCAATCGGCGGTGAGCCGCCAGATCCAGGCACTGG
AAGACGAGGTGGGCGTGCCGCTCTTCCTGCGGCACACGCGCGCGGTGGAGCTCACGAGCGCGGGGGCGCAACTGCAGCGC
GCCGTGGCACCGGCCCTGGAAAGGCTCGATGCCAGCGTGCGGCTCGTGCGGCAGACCGCCGGGCGCAAGAGCGTGGCGAT
CACGACCTGGGCCAGCTTCGCCTCCATGTGGCTGATTCCGCGCATGGAGGAATTCCAGCGCGACAACCCCGACATCGACA
TCCGCATCGATGCGAGCGACGTGTCCGTGGACCTGGAAACCGCGGACGTCGATCTGGCATTGCGCTATGCCGTGCCGGGC
TCGCAGCTGCATGGCGCGCAGCGGCTGTTCGGCGAGCAGCTCGCCGTGGTGGCCAGCCCGTGGCTGCTCAAGAGCGGCCC
GCCCATCCGCAGGCCCGCGGACGTCGCCCAGTTCACGCTGATCGAGGCCGGCGACGCCCACCGCATGGCCTACCTGGAAT
GGCTGACGTGGCGGCGGTGGTTCGAGCAGAACGGCCAATCCAAGCTGCAGCCCAAGCGCTGGCTCTACTTCAACTACGCC
CACCAGATCGTGCAGGCCGCGCTCACCGGCCAGGGCCTCGCGCTCGCACGGATGCCGCTCATCGCGGACAGCCTCGCTTC
CGGCGACCTCGTGGAGGTCCTGCCCGGCTACCGGCTCGACTCGCCGCTGGTGTACTGGCTGCTGGTGGGGCCGCGCAGCG
GGCAGCGCCCCGAGATCAAGGCGTTCTGCGCCTGGCTGCTGCGCGAGGCGCAACTCACCCGTGAAGCCGTGGGCGAAGTC
CCCGATCCGGACCTGAACGACGACCTGGGCTGA

Upstream 100 bases:

>100_bases
GAGTTTCTTTTCCAAAACAGATGTGGTCCAATGAAAACACGGCCATAATTTCATGCAATCGACTCATTAATCCAGCCGGC
GCAACCGGCATGCGAGCACC

Downstream 100 bases:

>100_bases
CCAGCGGCGGCACCCGGGCCCAGGCCCCGGGTTCTGGTGTCAGCCCAGCGTCACGCGGGCGAACTTGCGCTTGCCCACCT
GCACCACATAGGTACCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTIPAPVAHLRTRPVAVGHWRAFLAVARHLNFRAAAEELSLTQSAVSRQIQALEDEVGVPLFLRHTRAVELTSAGAQLQR
AVAPALERLDASVRLVRQTAGRKSVAITTWASFASMWLIPRMEEFQRDNPDIDIRIDASDVSVDLETADVDLALRYAVPG
SQLHGAQRLFGEQLAVVASPWLLKSGPPIRRPADVAQFTLIEAGDAHRMAYLEWLTWRRWFEQNGQSKLQPKRWLYFNYA
HQIVQAALTGQGLALARMPLIADSLASGDLVEVLPGYRLDSPLVYWLLVGPRSGQRPEIKAFCAWLLREAQLTREAVGEV
PDPDLNDDLG

Sequences:

>Translated_330_residues
MTIPAPVAHLRTRPVAVGHWRAFLAVARHLNFRAAAEELSLTQSAVSRQIQALEDEVGVPLFLRHTRAVELTSAGAQLQR
AVAPALERLDASVRLVRQTAGRKSVAITTWASFASMWLIPRMEEFQRDNPDIDIRIDASDVSVDLETADVDLALRYAVPG
SQLHGAQRLFGEQLAVVASPWLLKSGPPIRRPADVAQFTLIEAGDAHRMAYLEWLTWRRWFEQNGQSKLQPKRWLYFNYA
HQIVQAALTGQGLALARMPLIADSLASGDLVEVLPGYRLDSPLVYWLLVGPRSGQRPEIKAFCAWLLREAQLTREAVGEV
PDPDLNDDLG
>Mature_329_residues
TIPAPVAHLRTRPVAVGHWRAFLAVARHLNFRAAAEELSLTQSAVSRQIQALEDEVGVPLFLRHTRAVELTSAGAQLQRA
VAPALERLDASVRLVRQTAGRKSVAITTWASFASMWLIPRMEEFQRDNPDIDIRIDASDVSVDLETADVDLALRYAVPGS
QLHGAQRLFGEQLAVVASPWLLKSGPPIRRPADVAQFTLIEAGDAHRMAYLEWLTWRRWFEQNGQSKLQPKRWLYFNYAH
QIVQAALTGQGLALARMPLIADSLASGDLVEVLPGYRLDSPLVYWLLVGPRSGQRPEIKAFCAWLLREAQLTREAVGEVP
DPDLNDDLG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=33.8926174496644, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=29.7297297297297, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=31.7567567567568, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.0102389078498, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=27.7580071174377, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786401, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=25.2595155709343, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789440, Length=254, Percent_Identity=27.5590551181102, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=120, Percent_Identity=37.5, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=173, Percent_Identity=28.3236994219653, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081978, Length=264, Percent_Identity=28.7878787878788, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI2367136, Length=118, Percent_Identity=37.2881355932203, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=27.2357723577236, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787879, Length=160, Percent_Identity=31.25, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36744; Mature: 36612

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPAPVAHLRTRPVAVGHWRAFLAVARHLNFRAAAEELSLTQSAVSRQIQALEDEVGVP
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LFLRHTRAVELTSAGAQLQRAVAPALERLDASVRLVRQTAGRKSVAITTWASFASMWLIP
EEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
RMEEFQRDNPDIDIRIDASDVSVDLETADVDLALRYAVPGSQLHGAQRLFGEQLAVVASP
CHHHHCCCCCCEEEEEECCCCEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCC
WLLKSGPPIRRPADVAQFTLIEAGDAHRMAYLEWLTWRRWFEQNGQSKLQPKRWLYFNYA
HHCCCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHH
HQIVQAALTGQGLALARMPLIADSLASGDLVEVLPGYRLDSPLVYWLLVGPRSGQRPEIK
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCHHH
AFCAWLLREAQLTREAVGEVPDPDLNDDLG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TIPAPVAHLRTRPVAVGHWRAFLAVARHLNFRAAAEELSLTQSAVSRQIQALEDEVGVP
CCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LFLRHTRAVELTSAGAQLQRAVAPALERLDASVRLVRQTAGRKSVAITTWASFASMWLIP
EEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
RMEEFQRDNPDIDIRIDASDVSVDLETADVDLALRYAVPGSQLHGAQRLFGEQLAVVASP
CHHHHCCCCCCEEEEEECCCCEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCC
WLLKSGPPIRRPADVAQFTLIEAGDAHRMAYLEWLTWRRWFEQNGQSKLQPKRWLYFNYA
HHCCCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHH
HQIVQAALTGQGLALARMPLIADSLASGDLVEVLPGYRLDSPLVYWLLVGPRSGQRPEIK
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCHHH
AFCAWLLREAQLTREAVGEVPDPDLNDDLG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]