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The map label for this gene is nfnB [H]

Identifier: 120612656

GI number: 120612656

Start: 4467251

End: 4467886

Strand: Reverse

Name: nfnB [H]

Synonym: Aave_4019

Alternate gene names: 120612656

Gene position: 4467886-4467251 (Counterclockwise)

Preceding gene: 120612657

Following gene: 120612651

Centisome position: 83.47

GC content: 69.5

Gene sequence:

>636_bases
ATGGAATTGCTCGACGTGCTGCAGTGGCGCTACGCCACGAAGAAGATGGACCCCGCCCGCGCCGTGCCGCAGGACAAGGT
GGACCGCATCGTGGAGGCGGCCCGGCTGGCGCCCACGTCCAGCGGCCTGCAGCCGTTCGAGATCATCGTGGTGACGAACC
CCGAAGTGCGCGCCCGCATCCGCGAGGTGGCGTGGAACCAGGCGCAGGTGACGGACGGCTCGCACCTGCTGGTGTTCGCG
GCGTGGGACGACTACACGGCCGAGCGCATCAACGCGATGTTCGACTACACCAACGAGGTGCGGGGCTTCCGCAACGAAGG
CTGGGAGAACTACCGCAACATGCTGCTGGGCTCGTACCCGCAGCGCGGCGCGGAGGTGAACTTCCAGCATGCCGCGCGGC
AGGCCTACATCGGCTTCGGCGCGGCGATCATCGCCGCGGCGGCCGAGCAGGTGGACAGCACGCCCATGGAGGGCTTCCAG
CCCGAGGCGGTGGACGAGATCCTGGGCCTGCGCGCACGCGGCCTGCGCAGCGTGCTGCTGCTGCCGCTGGGCTACCGCCA
GCCCGAGGGCGACTGGCTGGTGAACCTGGAGAAGGTGCGCCGGCCGCGGGAGGAATTCGTTTCCGAAGTGCGCTGA

Upstream 100 bases:

>100_bases
GCGTCCTTGCGTGCAATTCCCCGTGCGAGCGCGGCCTCGCGGTCGCTCCTACACTCGGCCGTTCCTGACTCCTCAACCCC
TACAACCAGGACCCAGAACC

Downstream 100 bases:

>100_bases
ACCTCTGACGAAGTCGGCGGCAGCGTCGGCGGCATGTGGTGCCGCCGTCGTTGCCGCGGCACGGTCAGAACAGCCGCCGC
ACCAGCGCGATCAGCGCCAG

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MELLDVLQWRYATKKMDPARAVPQDKVDRIVEAARLAPTSSGLQPFEIIVVTNPEVRARIREVAWNQAQVTDGSHLLVFA
AWDDYTAERINAMFDYTNEVRGFRNEGWENYRNMLLGSYPQRGAEVNFQHAARQAYIGFGAAIIAAAAEQVDSTPMEGFQ
PEAVDEILGLRARGLRSVLLLPLGYRQPEGDWLVNLEKVRRPREEFVSEVR

Sequences:

>Translated_211_residues
MELLDVLQWRYATKKMDPARAVPQDKVDRIVEAARLAPTSSGLQPFEIIVVTNPEVRARIREVAWNQAQVTDGSHLLVFA
AWDDYTAERINAMFDYTNEVRGFRNEGWENYRNMLLGSYPQRGAEVNFQHAARQAYIGFGAAIIAAAAEQVDSTPMEGFQ
PEAVDEILGLRARGLRSVLLLPLGYRQPEGDWLVNLEKVRRPREEFVSEVR
>Mature_211_residues
MELLDVLQWRYATKKMDPARAVPQDKVDRIVEAARLAPTSSGLQPFEIIVVTNPEVRARIREVAWNQAQVTDGSHLLVFA
AWDDYTAERINAMFDYTNEVRGFRNEGWENYRNMLLGSYPQRGAEVNFQHAARQAYIGFGAAIIAAAAEQVDSTPMEGFQ
PEAVDEILGLRARGLRSVLLLPLGYRQPEGDWLVNLEKVRRPREEFVSEVR

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=223, Percent_Identity=25.1121076233184, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23990; Mature: 23990

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLDVLQWRYATKKMDPARAVPQDKVDRIVEAARLAPTSSGLQPFEIIVVTNPEVRARI
CCHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
REVAWNQAQVTDGSHLLVFAAWDDYTAERINAMFDYTNEVRGFRNEGWENYRNMLLGSYP
HHHHCCCCEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHCCCC
QRGAEVNFQHAARQAYIGFGAAIIAAAAEQVDSTPMEGFQPEAVDEILGLRARGLRSVLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LPLGYRQPEGDWLVNLEKVRRPREEFVSEVR
HCCCCCCCCCCEEEEHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MELLDVLQWRYATKKMDPARAVPQDKVDRIVEAARLAPTSSGLQPFEIIVVTNPEVRARI
CCHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
REVAWNQAQVTDGSHLLVFAAWDDYTAERINAMFDYTNEVRGFRNEGWENYRNMLLGSYP
HHHHCCCCEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHCCCC
QRGAEVNFQHAARQAYIGFGAAIIAAAAEQVDSTPMEGFQPEAVDEILGLRARGLRSVLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LPLGYRQPEGDWLVNLEKVRRPREEFVSEVR
HCCCCCCCCCCEEEEHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609 [H]