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The map label for this gene is alkB [H]

Identifier: 120612626

GI number: 120612626

Start: 4421957

End: 4422631

Strand: Direct

Name: alkB [H]

Synonym: Aave_3988

Alternate gene names: 120612626

Gene position: 4421957-4422631 (Clockwise)

Preceding gene: 120612624

Following gene: 120612627

Centisome position: 82.61

GC content: 75.7

Gene sequence:

>675_bases
ATGCCCCGATCATCCGCCACGACACCCGATCTCTTCGACGGCGCACCCGCCGGGGACCGCGCCGCCCCGCTCCCGCTGGG
CCCGGGCGCGGCGCTGCTGCGCGGGTTCGCGCTGCCGGTGGCGGCGACGCTGCGCGAGGAGGTGCTGGCCGTGGCCCGCG
CCGCGCCCTGGCGGCACATGGAAACCCCGGGCGGGCGGGCGATGTCGGTCGCCACCACGTCCTGCGGCCGCCTGGGCTGG
GTGAGCGACCGCCGCGGCTACCGCTATGCCCCGCTCGACCCTGGCTCCGGCACGGCCTGGCCCGCCATGCCGGACGCCCT
CCGGCGGCTGGCCCGTGAAGCAGCCGCGCACGCCGGATTCCCGGATTTCGAGCCCGACAGCTGCCTGGTCAACCGCTACG
CCCCCGGCGCGCGCCTGTCGCTGCACCAGGACCGGGACGAGCACGACCTGCAGGCGCCCATCGTGTCGGTGTCGCTCGGG
CTCCCCGCGGTTTTCCTGTGGGGCGGCTTCGCGCGCACCGGCCCGGTGGCGCGCGTGCCGCTGCAACATGGCGACGTGGT
GGTGTGGGGTGGACCGGACCGGCTGCGCTTTCACGGCGTGCAGCCGGTGAAGGACGGCCTGCATCCGGAATGGGGCGCCG
AACGGGTGAACCTCACCTTCCGCAAGGCGGGCTGA

Upstream 100 bases:

>100_bases
ATGGATGGATCTCCTTGGAGGAAACGTGGGATACGCAGGAGCATGGCAGGACGCGAGAGCCCTTGCAGGACCGCGGCAAA
GACGCCATGTGGTCCATACC

Downstream 100 bases:

>100_bases
ACGGCAAAGGACTAGCTGAAACTCCGGCTGCGGCCGAACCAGTGGCTGGCCGCCCGGCGGGCCTCGTCCGCCATCCACTG
GGCCACGCCCGCGGCATATT

Product: DNA-N1-methyladenine dioxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPRSSATTPDLFDGAPAGDRAAPLPLGPGAALLRGFALPVAATLREEVLAVARAAPWRHMETPGGRAMSVATTSCGRLGW
VSDRRGYRYAPLDPGSGTAWPAMPDALRRLAREAAAHAGFPDFEPDSCLVNRYAPGARLSLHQDRDEHDLQAPIVSVSLG
LPAVFLWGGFARTGPVARVPLQHGDVVVWGGPDRLRFHGVQPVKDGLHPEWGAERVNLTFRKAG

Sequences:

>Translated_224_residues
MPRSSATTPDLFDGAPAGDRAAPLPLGPGAALLRGFALPVAATLREEVLAVARAAPWRHMETPGGRAMSVATTSCGRLGW
VSDRRGYRYAPLDPGSGTAWPAMPDALRRLAREAAAHAGFPDFEPDSCLVNRYAPGARLSLHQDRDEHDLQAPIVSVSLG
LPAVFLWGGFARTGPVARVPLQHGDVVVWGGPDRLRFHGVQPVKDGLHPEWGAERVNLTFRKAG
>Mature_223_residues
PRSSATTPDLFDGAPAGDRAAPLPLGPGAALLRGFALPVAATLREEVLAVARAAPWRHMETPGGRAMSVATTSCGRLGWV
SDRRGYRYAPLDPGSGTAWPAMPDALRRLAREAAAHAGFPDFEPDSCLVNRYAPGARLSLHQDRDEHDLQAPIVSVSLGL
PAVFLWGGFARTGPVARVPLQHGDVVVWGGPDRLRFHGVQPVKDGLHPEWGAERVNLTFRKAG

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=125, Percent_Identity=32.8, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1788541, Length=200, Percent_Identity=58, Blast_Score=247, Evalue=4e-67,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=37.7551020408163, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23914; Mature: 23783

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSSATTPDLFDGAPAGDRAAPLPLGPGAALLRGFALPVAATLREEVLAVARAAPWRHM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ETPGGRAMSVATTSCGRLGWVSDRRGYRYAPLDPGSGTAWPAMPDALRRLAREAAAHAGF
CCCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PDFEPDSCLVNRYAPGARLSLHQDRDEHDLQAPIVSVSLGLPAVFLWGGFARTGPVARVP
CCCCCCCCEEECCCCCCEEEECCCCCCCHHHCCEEEEECCCCEEEHHCCCCCCCCEEECC
LQHGDVVVWGGPDRLRFHGVQPVKDGLHPEWGAERVNLTFRKAG
CCCCCEEEECCCCCEEECCCCHHHHCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
PRSSATTPDLFDGAPAGDRAAPLPLGPGAALLRGFALPVAATLREEVLAVARAAPWRHM
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ETPGGRAMSVATTSCGRLGWVSDRRGYRYAPLDPGSGTAWPAMPDALRRLAREAAAHAGF
CCCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PDFEPDSCLVNRYAPGARLSLHQDRDEHDLQAPIVSVSLGLPAVFLWGGFARTGPVARVP
CCCCCCCCEEECCCCCCEEEECCCCCCCHHHCCEEEEECCCCEEEHHCCCCCCCCEEECC
LQHGDVVVWGGPDRLRFHGVQPVKDGLHPEWGAERVNLTFRKAG
CCCCCEEEECCCCCEEECCCCHHHHCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]