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The map label for this gene is outF [H]

Identifier: 120612613

GI number: 120612613

Start: 4404615

End: 4405850

Strand: Direct

Name: outF [H]

Synonym: Aave_3974

Alternate gene names: 120612613

Gene position: 4404615-4405850 (Clockwise)

Preceding gene: 120612612

Following gene: 120612614

Centisome position: 82.29

GC content: 72.33

Gene sequence:

>1236_bases
ATGCCCGCGTTTTCTTTCGAAGCCCTCGATGCCCAGGGGCAGACCCGCAAAGGCACCCTCGAAGCCGACAACGCCAAGGC
GGCGCGCAGCCTGCTGCGCGCCCAGGCGCTGGTGCCGCTGGCCGTGGAGGCGCTCGGCACCGGCCAGGGCGCGGGCGGAG
ACGGCAGCGTCTCGCTCGGTCAGCGGCTGTTCACGCGGCCGGTGTTCGGCGCCACGGGCCTGGCGGTGTGGACGCGCCAG
CTCGCGGGGCTCGTGTCGTCCGGGCTGCCGCTGGAGCGGGCGCTCACGGCGCTGGCCGACGAGTCGGACGACGACCGGCA
GCGCCACCTGGTGGCCACATTGCGGGCCGAGGTGAATGCCGGCTCCACCTTCGCGCGGGCGCTCACGGCGCACCCGCGGG
AGTTCTCGTCCATCTATTGCGCGGTGATCGGCGCCGGCGAATCCAGCGGCCACCTGGGGCTGGTGCTGGAGCGCCTGGCG
GACGACCTCGAAGAGCGGCAGGCGCTCAAGGCCAAGCTCATCGGCGCGTCCCTCTATCCGGCGATCGTGACGGTGATCGC
GATCGTGATCGTGCTGTTCCTCGTGGGCTACGTGGTGCCGCAGGTGGCCGCGGTGTTCGCGGGCAGCAAGCGCGCCCTGC
CTTTCCTCACCGTGGCGATGATGGCCCTCTCCGACGCGGTGCGCAGCTACGGCTGGGCGGCCCTGGTGGTGCTGGTGGTG
CTGGCGCTGGCCGCCCGCTCCGCGCTGGCCCAGCCCCGCGTGCGCGAACGCTTCGATGCCGCCTGGCTGGGCCTGCCCGT
GGTGGGCCGGCTCTCGCGCGGCTACAACGCGGCCCGTTTCACGGGCACGCTGTCGATGCTGGCCGGCGCGGGCGTGCCCA
TCCTGAAGGCCCTGCAGGCCGCCGCCGAGACGCTAGGCAACACCGCCATGCGCGCGGACGCGCTCGATGCGCTGGTGCTG
GTGCGCGAGGGCGCGCCCCTGGCCTCCGCGCTGGCGCAAAAAAAACGGTTCCCCGGCCCGGTGTCGATGTTCGCGCGCCT
GGGCGAGCAGACGGGGCAGTTGCCGCTGATGCTGCAGCGCGCGGCCGCGCAGCTGTCGGCCGAGGTGCAGCGCCGCGCGA
TGCAGCTGGCCACCATCCTGGAGCCCCTGCTCATCGTGGCGATGGGCGTGATCGTGATGCTCATCGTGCTGGCGGTGCTC
ATGCCCATCATCCAGCTCAACCAGTTCGTGAAGTAG

Upstream 100 bases:

>100_bases
GGGGCGCGGTCCCGTCGCCGGCAGCGGCTACCATCTCCGTCCGTCGCGCCGCTGGCGCCCCGTTTCCGGTCCGACCGCCC
TCCCCGAATTTCCTGTCCGC

Downstream 100 bases:

>100_bases
CCCCGCCATGGCCCTGACCCTGGACGACAAGCTCGTCGTCGCCATCTCCTCCCGGGCGCTCTTCGATTTCGAGGAAGAGA
ACCGCCTGTTCGAGGCCGGC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPAFSFEALDAQGQTRKGTLEADNAKAARSLLRAQALVPLAVEALGTGQGAGGDGSVSLGQRLFTRPVFGATGLAVWTRQ
LAGLVSSGLPLERALTALADESDDDRQRHLVATLRAEVNAGSTFARALTAHPREFSSIYCAVIGAGESSGHLGLVLERLA
DDLEERQALKAKLIGASLYPAIVTVIAIVIVLFLVGYVVPQVAAVFAGSKRALPFLTVAMMALSDAVRSYGWAALVVLVV
LALAARSALAQPRVRERFDAAWLGLPVVGRLSRGYNAARFTGTLSMLAGAGVPILKALQAAAETLGNTAMRADALDALVL
VREGAPLASALAQKKRFPGPVSMFARLGEQTGQLPLMLQRAAAQLSAEVQRRAMQLATILEPLLIVAMGVIVMLIVLAVL
MPIIQLNQFVK

Sequences:

>Translated_411_residues
MPAFSFEALDAQGQTRKGTLEADNAKAARSLLRAQALVPLAVEALGTGQGAGGDGSVSLGQRLFTRPVFGATGLAVWTRQ
LAGLVSSGLPLERALTALADESDDDRQRHLVATLRAEVNAGSTFARALTAHPREFSSIYCAVIGAGESSGHLGLVLERLA
DDLEERQALKAKLIGASLYPAIVTVIAIVIVLFLVGYVVPQVAAVFAGSKRALPFLTVAMMALSDAVRSYGWAALVVLVV
LALAARSALAQPRVRERFDAAWLGLPVVGRLSRGYNAARFTGTLSMLAGAGVPILKALQAAAETLGNTAMRADALDALVL
VREGAPLASALAQKKRFPGPVSMFARLGEQTGQLPLMLQRAAAQLSAEVQRRAMQLATILEPLLIVAMGVIVMLIVLAVL
MPIIQLNQFVK
>Mature_410_residues
PAFSFEALDAQGQTRKGTLEADNAKAARSLLRAQALVPLAVEALGTGQGAGGDGSVSLGQRLFTRPVFGATGLAVWTRQL
AGLVSSGLPLERALTALADESDDDRQRHLVATLRAEVNAGSTFARALTAHPREFSSIYCAVIGAGESSGHLGLVLERLAD
DLEERQALKAKLIGASLYPAIVTVIAIVIVLFLVGYVVPQVAAVFAGSKRALPFLTVAMMALSDAVRSYGWAALVVLVVL
ALAARSALAQPRVRERFDAAWLGLPVVGRLSRGYNAARFTGTLSMLAGAGVPILKALQAAAETLGNTAMRADALDALVLV
REGAPLASALAQKKRFPGPVSMFARLGEQTGQLPLMLQRAAAQLSAEVQRRAMQLATILEPLLIVAMGVIVMLIVLAVLM
PIIQLNQFVK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=408, Percent_Identity=32.5980392156863, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786295, Length=413, Percent_Identity=26.3922518159806, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43169; Mature: 43038

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFSFEALDAQGQTRKGTLEADNAKAARSLLRAQALVPLAVEALGTGQGAGGDGSVSLG
CCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QRLFTRPVFGATGLAVWTRQLAGLVSSGLPLERALTALADESDDDRQRHLVATLRAEVNA
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GSTFARALTAHPREFSSIYCAVIGAGESSGHLGLVLERLADDLEERQALKAKLIGASLYP
CHHHHHHHHCCCHHHHHEEEHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVTVIAIVIVLFLVGYVVPQVAAVFAGSKRALPFLTVAMMALSDAVRSYGWAALVVLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALAARSALAQPRVRERFDAAWLGLPVVGRLSRGYNAARFTGTLSMLAGAGVPILKALQA
HHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
AAETLGNTAMRADALDALVLVREGAPLASALAQKKRFPGPVSMFARLGEQTGQLPLMLQR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
AAAQLSAEVQRRAMQLATILEPLLIVAMGVIVMLIVLAVLMPIIQLNQFVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAFSFEALDAQGQTRKGTLEADNAKAARSLLRAQALVPLAVEALGTGQGAGGDGSVSLG
CCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QRLFTRPVFGATGLAVWTRQLAGLVSSGLPLERALTALADESDDDRQRHLVATLRAEVNA
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GSTFARALTAHPREFSSIYCAVIGAGESSGHLGLVLERLADDLEERQALKAKLIGASLYP
CHHHHHHHHCCCHHHHHEEEHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVTVIAIVIVLFLVGYVVPQVAAVFAGSKRALPFLTVAMMALSDAVRSYGWAALVVLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALAARSALAQPRVRERFDAAWLGLPVVGRLSRGYNAARFTGTLSMLAGAGVPILKALQA
HHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
AAETLGNTAMRADALDALVLVREGAPLASALAQKKRFPGPVSMFARLGEQTGQLPLMLQR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
AAAQLSAEVQRRAMQLATILEPLLIVAMGVIVMLIVLAVLMPIIQLNQFVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]