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The map label for this gene is gatA2 [H]

Identifier: 120612456

GI number: 120612456

Start: 4236369

End: 4237784

Strand: Reverse

Name: gatA2 [H]

Synonym: Aave_3815

Alternate gene names: 120612456

Gene position: 4237784-4236369 (Counterclockwise)

Preceding gene: 120612457

Following gene: 120612455

Centisome position: 79.17

GC content: 69.77

Gene sequence:

>1416_bases
ATGATGAGCGGCGAGATCATCGAACTGACGGCCCATGGGTTGACGAGTGCCTACCGAACCCTGGCGCTTTCTCCGGTGGA
AGCCATGCGCGCAGTGCTCGACCGGGTGCACGAGGTGGACGGCGACGTCAATGCATTCTGCGCGTTGGACGAAGACGGCG
CGATGCGCAGCGCCGCCGAATCCGAGCAGCGCTGGCTGCGCGGCGAACCGTTGGGGCCCCTGGACGGCGTGCCCGTCTCG
GTCAAGGACATGGTCGCCACGCGGGGCATGGCCACGCGCTTCGGTTCGCGCACGCTGCCGGAGGACCATCTCGTCGAGGT
GGATGCGCCCTGCATTCAGGAGCTGCGCCGCGCCGGCGCGCTGATCTTCGGCAAGACCACGACATCGGAGTTCGGCAACA
AGATCGTGGGAGACTGCCCGCTCACCGGCATCACCCGCAATCCCTGGAACCTCGACCGCACCGCTGGAGGCAGTAGCGGC
GGCGCGGGCGCGGCCCTGGCCGCGCGGATGGGGCCCCTGGCGGTGGGATCGGACGGCGGTGGCTCCATCCGCGTGCCCTC
GGCGTGGAACGGCGTCTGCGGGCTGAAGCCGTCCTTCAAGCGCGTGCCGTCCATGGACACGCAGAATGTGGGCGAGGTGG
CCAATATCGGTCCCATGGCCCGGGATGTGCGCGATCTCGCGACCCTGCTGGGCGCCATGGCGCGGCCATGGCCGCGCGAC
TGGCAGGCTTCGGCGCCCGCAGAGGATTTCCTCGCCGGGCTGGAAGCGGGCGTCGAGGGATTGCGCATCGCGTTCAGCAC
CGATCTGGGCATCGTCGGCGTGGCGCCGGAAACCGCCCGCGCCGCGGAGCGGGCCGCCGGACTGCTGGAAACGCTGGGCG
CCCGGGTGACCCGGCTGGGGCGGCTCGCTCCGCTCGATGACTATATGCGCAGCGGGATGCACACCGTCCTGTGGACTGCG
CGGCTGGAGCATCTGGTGCGCGGCACCCCCGCGCACCTGCAGGACCGCTTCGATCCGGAGATATTGAATCTCGTCACTGC
AGGGCGCGGCATGCTGGCCTCGGAACTCATGGATGCGATGATCGCGCGGCTGAAGCTCGCGAGCGCCATGCATGCCTTCT
TCGAGGACTACGACGTGCTGCTCTGCCCCACCTTCCACTGCCCGCCGCCGGCCGTGCCGGGCCTGCCTCCGGAACTGCGC
ACGGCGCCGCCGCTGACGTCCTGGTGCAACCATACGCAGCAGCCTGCTGCCAGCATTCCCTGTGGCCTGTCGTCGGACGG
CCTGCCCATCGGACTGCAGGTGATCGGACCGCGCATGCACGATGCGCTGGTGCTGCGCGTGTGCCGCGCCTATGAGCGGG
CTCGCGGAGAGTTCCCCGCGCCGCCGCTGGATGTGCGCAGGAAGGTGGCGCAATGA

Upstream 100 bases:

>100_bases
CTCACCTCCAATGCCAGACCATGATCGGCCGCGCCCGCAAGGATGGCGGCTTCGACCTGCTCGGGGCGGCTCCCTCCATC
CAGGCGCAATGCGGAGCAGC

Downstream 100 bases:

>100_bases
AGATCCGCAGGTTTTTCATCCAGAAGGAGACGATTCCGCCGGCGCCGCAGCGGCCAGCGGCGCGGCAGGTGCAGCGGGTC
GTCGCTGCCGCGGTGCTGGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MMSGEIIELTAHGLTSAYRTLALSPVEAMRAVLDRVHEVDGDVNAFCALDEDGAMRSAAESEQRWLRGEPLGPLDGVPVS
VKDMVATRGMATRFGSRTLPEDHLVEVDAPCIQELRRAGALIFGKTTTSEFGNKIVGDCPLTGITRNPWNLDRTAGGSSG
GAGAALAARMGPLAVGSDGGGSIRVPSAWNGVCGLKPSFKRVPSMDTQNVGEVANIGPMARDVRDLATLLGAMARPWPRD
WQASAPAEDFLAGLEAGVEGLRIAFSTDLGIVGVAPETARAAERAAGLLETLGARVTRLGRLAPLDDYMRSGMHTVLWTA
RLEHLVRGTPAHLQDRFDPEILNLVTAGRGMLASELMDAMIARLKLASAMHAFFEDYDVLLCPTFHCPPPAVPGLPPELR
TAPPLTSWCNHTQQPAASIPCGLSSDGLPIGLQVIGPRMHDALVLRVCRAYERARGEFPAPPLDVRRKVAQ

Sequences:

>Translated_471_residues
MMSGEIIELTAHGLTSAYRTLALSPVEAMRAVLDRVHEVDGDVNAFCALDEDGAMRSAAESEQRWLRGEPLGPLDGVPVS
VKDMVATRGMATRFGSRTLPEDHLVEVDAPCIQELRRAGALIFGKTTTSEFGNKIVGDCPLTGITRNPWNLDRTAGGSSG
GAGAALAARMGPLAVGSDGGGSIRVPSAWNGVCGLKPSFKRVPSMDTQNVGEVANIGPMARDVRDLATLLGAMARPWPRD
WQASAPAEDFLAGLEAGVEGLRIAFSTDLGIVGVAPETARAAERAAGLLETLGARVTRLGRLAPLDDYMRSGMHTVLWTA
RLEHLVRGTPAHLQDRFDPEILNLVTAGRGMLASELMDAMIARLKLASAMHAFFEDYDVLLCPTFHCPPPAVPGLPPELR
TAPPLTSWCNHTQQPAASIPCGLSSDGLPIGLQVIGPRMHDALVLRVCRAYERARGEFPAPPLDVRRKVAQ
>Mature_471_residues
MMSGEIIELTAHGLTSAYRTLALSPVEAMRAVLDRVHEVDGDVNAFCALDEDGAMRSAAESEQRWLRGEPLGPLDGVPVS
VKDMVATRGMATRFGSRTLPEDHLVEVDAPCIQELRRAGALIFGKTTTSEFGNKIVGDCPLTGITRNPWNLDRTAGGSSG
GAGAALAARMGPLAVGSDGGGSIRVPSAWNGVCGLKPSFKRVPSMDTQNVGEVANIGPMARDVRDLATLLGAMARPWPRD
WQASAPAEDFLAGLEAGVEGLRIAFSTDLGIVGVAPETARAAERAAGLLETLGARVTRLGRLAPLDDYMRSGMHTVLWTA
RLEHLVRGTPAHLQDRFDPEILNLVTAGRGMLASELMDAMIARLKLASAMHAFFEDYDVLLCPTFHCPPPAVPGLPPELR
TAPPLTSWCNHTQQPAASIPCGLSSDGLPIGLQVIGPRMHDALVLRVCRAYERARGEFPAPPLDVRRKVAQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=507, Percent_Identity=28.9940828402367, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI195972892, Length=243, Percent_Identity=31.6872427983539, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI166795287, Length=208, Percent_Identity=33.6538461538462, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=500, Percent_Identity=25.6, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=295, Percent_Identity=27.4576271186441, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=515, Percent_Identity=23.3009708737864, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=306, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=306, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=420, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=241, Percent_Identity=30.2904564315353, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=509, Percent_Identity=24.3614931237721, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=520, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319685, Length=397, Percent_Identity=26.1964735516373, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=420, Percent_Identity=23.5714285714286, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45550774, Length=494, Percent_Identity=27.1255060728745, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24648435, Length=494, Percent_Identity=27.1255060728745, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648437, Length=494, Percent_Identity=27.1255060728745, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648441, Length=420, Percent_Identity=28.3333333333333, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648439, Length=420, Percent_Identity=28.3333333333333, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=27.3084479371316, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24652985, Length=512, Percent_Identity=28.125, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19922090, Length=512, Percent_Identity=28.125, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652981, Length=512, Percent_Identity=28.125, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652983, Length=512, Percent_Identity=28.125, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24648113, Length=505, Percent_Identity=28.5148514851485, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=25.1984126984127, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24644968, Length=489, Percent_Identity=24.7443762781186, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50189; Mature: 50189

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSGEIIELTAHGLTSAYRTLALSPVEAMRAVLDRVHEVDGDVNAFCALDEDGAMRSAAE
CCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
SEQRWLRGEPLGPLDGVPVSVKDMVATRGMATRFGSRTLPEDHLVEVDAPCIQELRRAGA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHEEECCCHHHHHHHHCCC
LIFGKTTTSEFGNKIVGDCPLTGITRNPWNLDRTAGGSSGGAGAALAARMGPLAVGSDGG
EEEECCCHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCC
GSIRVPSAWNGVCGLKPSFKRVPSMDTQNVGEVANIGPMARDVRDLATLLGAMARPWPRD
CEEECCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
WQASAPAEDFLAGLEAGVEGLRIAFSTDLGIVGVAPETARAAERAAGLLETLGARVTRLG
CCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RLAPLDDYMRSGMHTVLWTARLEHLVRGTPAHLQDRFDPEILNLVTAGRGMLASELMDAM
CCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
IARLKLASAMHAFFEDYDVLLCPTFHCPPPAVPGLPPELRTAPPLTSWCNHTQQPAASIP
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHCCC
CGLSSDGLPIGLQVIGPRMHDALVLRVCRAYERARGEFPAPPLDVRRKVAQ
CCCCCCCCCEEHEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MMSGEIIELTAHGLTSAYRTLALSPVEAMRAVLDRVHEVDGDVNAFCALDEDGAMRSAAE
CCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
SEQRWLRGEPLGPLDGVPVSVKDMVATRGMATRFGSRTLPEDHLVEVDAPCIQELRRAGA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHEEECCCHHHHHHHHCCC
LIFGKTTTSEFGNKIVGDCPLTGITRNPWNLDRTAGGSSGGAGAALAARMGPLAVGSDGG
EEEECCCHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCC
GSIRVPSAWNGVCGLKPSFKRVPSMDTQNVGEVANIGPMARDVRDLATLLGAMARPWPRD
CEEECCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
WQASAPAEDFLAGLEAGVEGLRIAFSTDLGIVGVAPETARAAERAAGLLETLGARVTRLG
CCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RLAPLDDYMRSGMHTVLWTARLEHLVRGTPAHLQDRFDPEILNLVTAGRGMLASELMDAM
CCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
IARLKLASAMHAFFEDYDVLLCPTFHCPPPAVPGLPPELRTAPPLTSWCNHTQQPAASIP
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHCCC
CGLSSDGLPIGLQVIGPRMHDALVLRVCRAYERARGEFPAPPLDVRRKVAQ
CCCCCCCCCEEHEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]