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The map label for this gene is psdht [H]

Identifier: 120612411

GI number: 120612411

Start: 4188459

End: 4189400

Strand: Reverse

Name: psdht [H]

Synonym: Aave_3768

Alternate gene names: 120612411

Gene position: 4189400-4188459 (Counterclockwise)

Preceding gene: 120612413

Following gene: 120612410

Centisome position: 78.27

GC content: 75.37

Gene sequence:

>942_bases
ATGATCGACCGTTCCGCCATTGCCTCCGCGCGGCAGCGCCTGACCGCCCATCCCGACTTCCTCCGCACCACGCCGCTCAT
GCGCCTTTCCGGACGCTCGGCGGGCCTGGGCTGCGCGGAGGTCTGGCTCAAGCTGGAGCACCTGCAGGTGGGCGGCAGCT
TCAAGGCCCGCGGCATGCTCAACCGCCTGCTGGCCCAGCCGCTGCCCGACAGCGGCGTGATCGTGGCCTCGGGCGGCAAT
GCCGGCATCGCCACCGCCGCCGCCGCGCGCTCGCTGGGCACGCGCTGCGAGGTGTTCGTGCCCGAGGTATCGCCCGAGGC
CAAGCGTGCGCGCCTGCGCGCCCTGGGGGCGCAGGTCGTCGTGGCCGGGCCGACCTACGCCGAGGCGCTGCAGGCCTGCC
TGGAGCGCCAGCGCGAGACCGGCGCGCTGCTCACCCACGCCTACGACCAGCCCGAAGTGGTGGCCGGTGCCGGCACCCTG
GCGCTGGAGATGGAAGAGCAGGCCCGGCGCCTGCCCGACAGCGTGCTCGTGAGCGTGGGCGGCGGCGGCCTGATCGGCGG
CATCGCCGCCTGGTGCGAGGGCCGCGCCCGCGTGGTCGCCCTGGAACCCGAGGGGGCGCCCACGCTGCACGGCGCGCGGG
CCGCGGGCATGCCCGTGGACGTGGCGGTGGGCGGCATCGCGGCCGATTCGCTCGGCGCCAAGCGCATCGGCGACATCGCC
TGGCAGGTGTCGCAGCGCCATGTGCAGGAGTCGCTGCTGGTGTCCGACGACGCCATCCGCGCGGCGCAGCAATGGCTGTG
GCACGAACTCAAGCTGGCGGTGGAGCCGGCGGCGGCGCTGGGCATCGCGGCCCTGCAGAGCGGCGTGTACCGCCCCCGGC
CCGACGAGACCGTGGGCCTGGTGCTCTGCGGCGCCAACTGCGACCCGGCCACCGTGGCCTGA

Upstream 100 bases:

>100_bases
TGTTATCGGCACCCGGCGCCGAAGGTGTGCATACACTGTCAGGTTTCGCCTCCGAAACCCGGGGCGCCGTTCCACCCGCA
TTTCTCCTGGTGTCCGCTTC

Downstream 100 bases:

>100_bases
GAACCCGGGCCCGCCGGGCGGGGGCGGGGCCGTAAAATCGCCGGCTATGCTCGACATCCTCCTCCTCCGCAAAGACCTCG
ATTCCGCCATCGCGCGGCTG

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIDRSAIASARQRLTAHPDFLRTTPLMRLSGRSAGLGCAEVWLKLEHLQVGGSFKARGMLNRLLAQPLPDSGVIVASGGN
AGIATAAAARSLGTRCEVFVPEVSPEAKRARLRALGAQVVVAGPTYAEALQACLERQRETGALLTHAYDQPEVVAGAGTL
ALEMEEQARRLPDSVLVSVGGGGLIGGIAAWCEGRARVVALEPEGAPTLHGARAAGMPVDVAVGGIAADSLGAKRIGDIA
WQVSQRHVQESLLVSDDAIRAAQQWLWHELKLAVEPAAALGIAALQSGVYRPRPDETVGLVLCGANCDPATVA

Sequences:

>Translated_313_residues
MIDRSAIASARQRLTAHPDFLRTTPLMRLSGRSAGLGCAEVWLKLEHLQVGGSFKARGMLNRLLAQPLPDSGVIVASGGN
AGIATAAAARSLGTRCEVFVPEVSPEAKRARLRALGAQVVVAGPTYAEALQACLERQRETGALLTHAYDQPEVVAGAGTL
ALEMEEQARRLPDSVLVSVGGGGLIGGIAAWCEGRARVVALEPEGAPTLHGARAAGMPVDVAVGGIAADSLGAKRIGDIA
WQVSQRHVQESLLVSDDAIRAAQQWLWHELKLAVEPAAALGIAALQSGVYRPRPDETVGLVLCGANCDPATVA
>Mature_313_residues
MIDRSAIASARQRLTAHPDFLRTTPLMRLSGRSAGLGCAEVWLKLEHLQVGGSFKARGMLNRLLAQPLPDSGVIVASGGN
AGIATAAAARSLGTRCEVFVPEVSPEAKRARLRALGAQVVVAGPTYAEALQACLERQRETGALLTHAYDQPEVVAGAGTL
ALEMEEQARRLPDSVLVSVGGGGLIGGIAAWCEGRARVVALEPEGAPTLHGARAAGMPVDVAVGGIAADSLGAKRIGDIA
WQVSQRHVQESLLVSDDAIRAAQQWLWHELKLAVEPAAALGIAALQSGVYRPRPDETVGLVLCGANCDPATVA

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=301, Percent_Identity=31.5614617940199, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI295821202, Length=211, Percent_Identity=31.2796208530806, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI295821200, Length=211, Percent_Identity=31.2796208530806, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4557415, Length=211, Percent_Identity=31.2796208530806, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI33469958, Length=282, Percent_Identity=29.0780141843972, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI19923959, Length=305, Percent_Identity=32.7868852459016, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1790207, Length=294, Percent_Identity=32.312925170068, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1789505, Length=277, Percent_Identity=30.3249097472924, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI2367138, Length=204, Percent_Identity=27.4509803921569, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17508781, Length=300, Percent_Identity=29.6666666666667, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71991565, Length=296, Percent_Identity=30.7432432432432, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537387, Length=280, Percent_Identity=31.7857142857143, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320930, Length=302, Percent_Identity=32.7814569536424, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319788, Length=279, Percent_Identity=28.673835125448, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322631, Length=298, Percent_Identity=28.8590604026846, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355833, Length=281, Percent_Identity=32.7402135231317, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 32527; Mature: 32527

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRSAIASARQRLTAHPDFLRTTPLMRLSGRSAGLGCAEVWLKLEHLQVGGSFKARGML
CCCHHHHHHHHHHHCCCCCHHHCCCHHEECCCCCCCCHHHHHHHHHHEEECCCCHHHHHH
NRLLAQPLPDSGVIVASGGNAGIATAAAARSLGTRCEVFVPEVSPEAKRARLRALGAQVV
HHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEE
VAGPTYAEALQACLERQRETGALLTHAYDQPEVVAGAGTLALEMEEQARRLPDSVLVSVG
EECCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCEEEEEEHHHHHHCCCCEEEECC
GGGLIGGIAAWCEGRARVVALEPEGAPTLHGARAAGMPVDVAVGGIAADSLGAKRIGDIA
CCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCEEHHHCCHHHHHHHH
WQVSQRHVQESLLVSDDAIRAAQQWLWHELKLAVEPAAALGIAALQSGVYRPRPDETVGL
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEE
VLCGANCDPATVA
EEECCCCCCCCCC
>Mature Secondary Structure
MIDRSAIASARQRLTAHPDFLRTTPLMRLSGRSAGLGCAEVWLKLEHLQVGGSFKARGML
CCCHHHHHHHHHHHCCCCCHHHCCCHHEECCCCCCCCHHHHHHHHHHEEECCCCHHHHHH
NRLLAQPLPDSGVIVASGGNAGIATAAAARSLGTRCEVFVPEVSPEAKRARLRALGAQVV
HHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEE
VAGPTYAEALQACLERQRETGALLTHAYDQPEVVAGAGTLALEMEEQARRLPDSVLVSVG
EECCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCEEEEEEHHHHHHCCCCEEEECC
GGGLIGGIAAWCEGRARVVALEPEGAPTLHGARAAGMPVDVAVGGIAADSLGAKRIGDIA
CCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCEEHHHCCHHHHHHHH
WQVSQRHVQESLLVSDDAIRAAQQWLWHELKLAVEPAAALGIAALQSGVYRPRPDETVGL
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEE
VLCGANCDPATVA
EEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]