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The map label for this gene is pilB [H]

Identifier: 120612324

GI number: 120612324

Start: 4057202

End: 4058938

Strand: Direct

Name: pilB [H]

Synonym: Aave_3680

Alternate gene names: 120612324

Gene position: 4057202-4058938 (Clockwise)

Preceding gene: 120612323

Following gene: 120612325

Centisome position: 75.8

GC content: 65.4

Gene sequence:

>1737_bases
ATGGCTGACGCCGATTCCGCCCTCAAGGACGCTTCCAGTATCGCTTTGCCCGGCCTGGGGCGGGCGCTGATTTCGGCCGG
GCAGCTCACCCAGAAGGTGGCGGAGGACATCTACAGGAAGTCCCAGGCCAACCGCAGCAGTTTCATCGCCGAGCTGACCG
GCTCAGGCTCGGTGTCCCCGATCAATCTGGCGCATACCGTGTCGTCGGTGTTCGGTGCCCCCCTGCTCGACATCGAGGCC
ATCGATCCGCTGCGCCTGCCGCGCGACGTGCTGGATCCGAAGATCTGCCAGGCGTACCGCGTCATCGTGCTGAGCAAGCG
CAACAACCGGCTCATCGTCGCGACGGCGGACCCGACCGACCAGGAGGCGGCGGAGAAGATCAAGTTCACCACCCAGATGG
GCGTGGACTGGATCATCGCCGAGTACGACAAGCTGGTGCGGCTGGTGGATGCGACCACCAAGTCCACGACCGAGACCATG
GAAAGCATGGTCTCCGGGGGCGATTTCGAGTTCGACGACCTGCCGGCGCAGGAAAGTACCGAAGCCCAGGACACGGCCGC
GACGGAGGTCGAAGACGCCCCCATCGTCAAGTTCCTGCACAAGATGCTGCTGGACGCGTTCAACATGCGCGCGTCCGATC
TGCACTTCGAGCCGTACGAGCACCAGTACCGGGTGCGCTTCCGGATCGACGGCGAGCTGCGCGAGATCGCGTCGCCGCCC
ATCGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGACATCTCGGAAAAGCGCGTTCCCCAGGA
CGGGCGCATGAAGCTCAAGGTGGGGCCGGACCGGGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGA
AGATCGTGATCCGTATCCTCGACCCCAGCAGCGCCAAGCTGGGCATCGACGCGCTCGGCTACGAGCCCGAGGAGAAGGAA
CGCCTGCTCCATGCCATCGGCCGGCCCTACGGCATGATCCTGGTGACGGGACCGACGGGCTCGGGCAAGACGGTGTCGCT
CTACACCTGCCTGAACCTGCTGAACAAGCCGGGGGTGAACATCGCGACGGCGGAAGACCCGTCCGAAATCAACCTGCCCG
GCGTCAACCAGGTCAACGTGAACGAGAAAGCCGGCCTGACCTTCGCCACGGCGCTGAAGTCGTTCCTGCGCCAGGATCCC
GACATCATCATGGTGGGGGAAATCCGCGACCTGGAAACCGCGGACATCTCGATCAAGGCGGCCCAGACGGGCCACCTCGT
GCTCTCCACGCTGCATACCAACGACGCGCCCACCACGCTGACGCGGATGCGGAACATGGGCATCGCGCCCTTCAACATCG
CGTCGAGCGTGATCCTGATCACGGCGCAGCGCCTGGCCCGCCGGCTGTGCCCCGCCTGCAAGGCGCCTGCGGACATCCCC
CACGAGACGCTGCTCGAGGCGGGCTACAGGGAAGAGGACATCGACGGCTCCTGGGTGACCTACCGCCCTGTGGGCTGCTC
TGCATGCAACAACGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGGTCTCGGAAGAGATCCAGCGCATCATCC
TGCGCGACGGCAGCGCGCTGGAGATCGCGGAGCAGGCGAAGGCCGAAGGCGTGCGGTCGCTGCGCGAATCCGGACTCCAC
AAGGCACGGATGGGCCTGACCTCGCTCGAAGAGGTGCTCGCCGTGACCAACGAATAA

Upstream 100 bases:

>100_bases
TGCCGCAAGCGGCGTCAATTGCCGGGCATGCCATGCACCAAGCGTTGCAACTGCAATGATAGGATGGGTTACTTTCCAAC
CTTTCGACTCTTCTGGTTAC

Downstream 100 bases:

>100_bases
CAACGCTGCAATCCATCTTCAAGGGGCCCGCATGGCAACCGCTGCATCGAGGGACATCAAGGATTTCGTTTTCGAGTGGG
AAGGCAAGGACCGCCACGGC

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MADADSALKDASSIALPGLGRALISAGQLTQKVAEDIYRKSQANRSSFIAELTGSGSVSPINLAHTVSSVFGAPLLDIEA
IDPLRLPRDVLDPKICQAYRVIVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLVRLVDATTKSTTETM
ESMVSGGDFEFDDLPAQESTEAQDTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKE
RLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADIP
HETLLEAGYREEDIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQAKAEGVRSLRESGLH
KARMGLTSLEEVLAVTNE

Sequences:

>Translated_578_residues
MADADSALKDASSIALPGLGRALISAGQLTQKVAEDIYRKSQANRSSFIAELTGSGSVSPINLAHTVSSVFGAPLLDIEA
IDPLRLPRDVLDPKICQAYRVIVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLVRLVDATTKSTTETM
ESMVSGGDFEFDDLPAQESTEAQDTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKE
RLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADIP
HETLLEAGYREEDIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQAKAEGVRSLRESGLH
KARMGLTSLEEVLAVTNE
>Mature_577_residues
ADADSALKDASSIALPGLGRALISAGQLTQKVAEDIYRKSQANRSSFIAELTGSGSVSPINLAHTVSSVFGAPLLDIEAI
DPLRLPRDVLDPKICQAYRVIVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLVRLVDATTKSTTETME
SMVSGGDFEFDDLPAQESTEAQDTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPI
AIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADIPH
ETLLEAGYREEDIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQAKAEGVRSLRESGLHK
ARMGLTSLEEVLAVTNE

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=411, Percent_Identity=45.0121654501217, Blast_Score=342, Evalue=5e-95,
Organism=Escherichia coli, GI1786296, Length=491, Percent_Identity=36.6598778004073, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI87082188, Length=117, Percent_Identity=44.4444444444444, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 63211; Mature: 63080

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADADSALKDASSIALPGLGRALISAGQLTQKVAEDIYRKSQANRSSFIAELTGSGSVSP
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCC
INLAHTVSSVFGAPLLDIEAIDPLRLPRDVLDPKICQAYRVIVLSKRNNRLIVATADPTD
CHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCHHHHHHEEEEEEECCCCEEEEEECCCCC
QEAAEKIKFTTQMGVDWIIAEYDKLVRLVDATTKSTTETMESMVSGGDFEFDDLPAQEST
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
EAQDTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHCCCC
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHCCCEEEEEEE
DPSSAKLGIDALGYEPEEKERLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVN
CCCCCCCCCHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCCE
IATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDPDIIMVGEIRDLETADISIKA
EEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
AQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADIP
ECCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCC
HETLLEAGYREEDIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSAL
HHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCHH
EIAEQAKAEGVRSLRESGLHKARMGLTSLEEVLAVTNE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADADSALKDASSIALPGLGRALISAGQLTQKVAEDIYRKSQANRSSFIAELTGSGSVSP
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCC
INLAHTVSSVFGAPLLDIEAIDPLRLPRDVLDPKICQAYRVIVLSKRNNRLIVATADPTD
CHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCHHHHHHEEEEEEECCCCEEEEEECCCCC
QEAAEKIKFTTQMGVDWIIAEYDKLVRLVDATTKSTTETMESMVSGGDFEFDDLPAQEST
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
EAQDTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHCCCC
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHCCCEEEEEEE
DPSSAKLGIDALGYEPEEKERLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVN
CCCCCCCCCHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCCE
IATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDPDIIMVGEIRDLETADISIKA
EEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
AQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADIP
ECCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCC
HETLLEAGYREEDIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSAL
HHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCHH
EIAEQAKAEGVRSLRESGLHKARMGLTSLEEVLAVTNE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]