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The map label for this gene is pleD [H]

Identifier: 120612291

GI number: 120612291

Start: 4019294

End: 4020367

Strand: Direct

Name: pleD [H]

Synonym: Aave_3646

Alternate gene names: 120612291

Gene position: 4019294-4020367 (Clockwise)

Preceding gene: 120612280

Following gene: 120612295

Centisome position: 75.09

GC content: 69.74

Gene sequence:

>1074_bases
ATGCCCTCCACCGACGCTTCCGCCATCACGGCCCCCCTCTCCCACCGGGAGGGGCACATGGCCCGCAAGTCGTTCTGGGT
GATGACCCAGCGGGTGACCGTGATGGCCGCGGCCGTGGACGCGGCCTTCATTCCCCTTTTCCTGATGCTCGGATCGCCGC
TGCTCACCTGGCTGAACCTGGTGAGCATCGGCATGTACGCCGCGGCCTACGCACTGCTGCAGCGGCACCGCAACGGCGCT
GCGCTGGCGCTGATCTGGCTCGAGGTGGTGGGCCACTCGGCCATCGGCACCTGGCTGATCGGCTGGAACAGCGGCTTCCA
CTATTACCTGCTGATGTTCATTCCCGCCATCGTGGTGGGCCGGGGCCGCCGCGCCGCCACGCCCGTGATGCTGTTTGCGC
TCTTCGTTTTCTACGTCGGCATGTACGAGTTTTCGCGCCGCCATGGCGCGCAGGCGCCGCTCGATCCGCTGGGACAGTCG
ATCGCGCATGCGTTCAACGTGGCGGTGGTATTCGCCATGGCGGCCTACACCGCCCGCTACTACTACGGCTCGGTGCTGCG
GGCCGAGCGCCGCCTGGAAGAGCAGGCGTGCACCGACCCGCTCACGGGGTTGTCCAACCGGCGGCGCCTCGCCGGCCTGG
CGCAGGAGCGCATCGCGCAGGCACGGGCCGCGGGCCAGCCGACCTCGGTGGTGATCGCCGACATCGACCACTTCAAACGC
ATCAACGACACCCATGGCCACGAAGCCGGTGACCAGGTCCTCGTGCACGTGGCGGAACTCATGGAGCGCAGCAGCCGCGC
GCACGACCTGCTGGCGCGCTGGGGCGGCGAGGAATTCCTCCTGGTGATGCCCTCGTGCGGCCTGGGCGACGCGCAGGCCC
TGGCCGAGCGCATGCGCGCCATCGTGGCCGGACATCCGGCCCGCCGCGACACGCAAGCCATCCCGGCGAGCCTATCGCTG
GGCGTGGCGCAGCTGCGCGGAGGCGAATCGCTGGAGGCCGCGATTGCCCGGGCCGACCAGGCGCTGTATGCCAGCAAGGA
AGCAGGCCGCAACCGCGTGACGACGGCGGACTGA

Upstream 100 bases:

>100_bases
TGGGAGATCCTCTCGTCCGCCACGCCCGGAGGCGGCGGTAGCAACTCTTACATTGGTGGACGAGAATTGTCCCACCAGCC
GCAGCCCGCCCCAGGCCACC

Downstream 100 bases:

>100_bases
ACGCGCCGAGAACACGGCCGGCCGGTGCTCCGGCGCGTATGCGCGGATTCAGTCCAGGCCGGCCTGCGCCAGGCGCGCGC
GCAGCTGCTGCGCGATGGAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPSTDASAITAPLSHREGHMARKSFWVMTQRVTVMAAAVDAAFIPLFLMLGSPLLTWLNLVSIGMYAAAYALLQRHRNGA
ALALIWLEVVGHSAIGTWLIGWNSGFHYYLLMFIPAIVVGRGRRAATPVMLFALFVFYVGMYEFSRRHGAQAPLDPLGQS
IAHAFNVAVVFAMAAYTARYYYGSVLRAERRLEEQACTDPLTGLSNRRRLAGLAQERIAQARAAGQPTSVVIADIDHFKR
INDTHGHEAGDQVLVHVAELMERSSRAHDLLARWGGEEFLLVMPSCGLGDAQALAERMRAIVAGHPARRDTQAIPASLSL
GVAQLRGGESLEAAIARADQALYASKEAGRNRVTTAD

Sequences:

>Translated_357_residues
MPSTDASAITAPLSHREGHMARKSFWVMTQRVTVMAAAVDAAFIPLFLMLGSPLLTWLNLVSIGMYAAAYALLQRHRNGA
ALALIWLEVVGHSAIGTWLIGWNSGFHYYLLMFIPAIVVGRGRRAATPVMLFALFVFYVGMYEFSRRHGAQAPLDPLGQS
IAHAFNVAVVFAMAAYTARYYYGSVLRAERRLEEQACTDPLTGLSNRRRLAGLAQERIAQARAAGQPTSVVIADIDHFKR
INDTHGHEAGDQVLVHVAELMERSSRAHDLLARWGGEEFLLVMPSCGLGDAQALAERMRAIVAGHPARRDTQAIPASLSL
GVAQLRGGESLEAAIARADQALYASKEAGRNRVTTAD
>Mature_356_residues
PSTDASAITAPLSHREGHMARKSFWVMTQRVTVMAAAVDAAFIPLFLMLGSPLLTWLNLVSIGMYAAAYALLQRHRNGAA
LALIWLEVVGHSAIGTWLIGWNSGFHYYLLMFIPAIVVGRGRRAATPVMLFALFVFYVGMYEFSRRHGAQAPLDPLGQSI
AHAFNVAVVFAMAAYTARYYYGSVLRAERRLEEQACTDPLTGLSNRRRLAGLAQERIAQARAAGQPTSVVIADIDHFKRI
NDTHGHEAGDQVLVHVAELMERSSRAHDLLARWGGEEFLLVMPSCGLGDAQALAERMRAIVAGHPARRDTQAIPASLSLG
VAQLRGGESLEAAIARADQALYASKEAGRNRVTTAD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=41.5204678362573, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787262, Length=176, Percent_Identity=38.6363636363636, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=40.3846153846154, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=38.3783783783784, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=33.5294117647059, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=33.9506172839506, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=33.7278106508876, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=31.0975609756098, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=32.9192546583851, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=37.3493975903614, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=25.748502994012, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=154, Percent_Identity=31.1688311688312, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38876; Mature: 38745

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTDASAITAPLSHREGHMARKSFWVMTQRVTVMAAAVDAAFIPLFLMLGSPLLTWLNL
CCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VSIGMYAAAYALLQRHRNGAALALIWLEVVGHSAIGTWLIGWNSGFHYYLLMFIPAIVVG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHEEEEEECCCHHHHHHHHHHHHHHC
RGRRAATPVMLFALFVFYVGMYEFSRRHGAQAPLDPLGQSIAHAFNVAVVFAMAAYTARY
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYGSVLRAERRLEEQACTDPLTGLSNRRRLAGLAQERIAQARAAGQPTSVVIADIDHFKR
HHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHH
INDTHGHEAGDQVLVHVAELMERSSRAHDLLARWGGEEFLLVMPSCGLGDAQALAERMRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
IVAGHPARRDTQAIPASLSLGVAQLRGGESLEAAIARADQALYASKEAGRNRVTTAD
HHHCCCCCCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PSTDASAITAPLSHREGHMARKSFWVMTQRVTVMAAAVDAAFIPLFLMLGSPLLTWLNL
CCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VSIGMYAAAYALLQRHRNGAALALIWLEVVGHSAIGTWLIGWNSGFHYYLLMFIPAIVVG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHEEEEEECCCHHHHHHHHHHHHHHC
RGRRAATPVMLFALFVFYVGMYEFSRRHGAQAPLDPLGQSIAHAFNVAVVFAMAAYTARY
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYGSVLRAERRLEEQACTDPLTGLSNRRRLAGLAQERIAQARAAGQPTSVVIADIDHFKR
HHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHH
INDTHGHEAGDQVLVHVAELMERSSRAHDLLARWGGEEFLLVMPSCGLGDAQALAERMRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
IVAGHPARRDTQAIPASLSLGVAQLRGGESLEAAIARADQALYASKEAGRNRVTTAD
HHHCCCCCCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA