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The map label for this gene is aspS

Identifier: 120612283

GI number: 120612283

Start: 4010063

End: 4011877

Strand: Reverse

Name: aspS

Synonym: Aave_3637

Alternate gene names: 120612283

Gene position: 4011877-4010063 (Counterclockwise)

Preceding gene: 120612284

Following gene: 120612282

Centisome position: 74.95

GC content: 66.56

Gene sequence:

>1815_bases
ATGGCCATGCGTTCCCACTATTGCGGTCTCGTCACCGAAGCCCTGATGGGCCAAACCGTCACCCTCGCGGGCTGGGTGAA
CCGCCGCCGCGACCATGGCGGCGTGATCTTCATCGACCTGCGCGACCGCGAAGGCAACGTGCAGGTGGTGTGCGACCCGG
ACCGCGCCGACATGTTCAAGACGGCCGAGGGCGTGCGCAACGAGTTCTGCGTGCAGGTCAAGGGCCTGGTGCGCGCGCGC
CCCGAGGGCACGACCAACGACAGCCTCAAGAGCGGCAGGATCGAGGTGCTGTGCCACGAGCTGAACGTGCTGAACCCCTC
GGTGACGCCCCCGTTCCAGATGGACGACGAGAACCTGTCGGAAACCACGCGCCTCACGCACCGCGTGCTCGACCTGCGCC
GCCCGTACATGCAGCGCAACCTGATGCTGCGCTACAAGACGGCGATCCAGGTGCGCAACTTCCTGGACAAGGAAGGCTTC
ATCGACATCGAGACGCCCATGCTGGGCAAGAGCACGCCCGAGGGCGCGCGCGACTACCTCGTGCCCAGCCGCGTGCACGA
CGGCCAGTTCTTCGCGCTGCCGCAGTCGCCCCAGCTCTACAAGCAGATGCTGATGGTGGCCGGCTACGACCGCTACTACC
AGATCACCAAGTGCTTCCGCGACGAGGACCTGCGCGCCGACCGCCAGCCCGAGTTCACGCAGATCGACTGCGAAACCTCG
TTCCTGAACGAGGAAGAAATCCGCGCGATCTTCCAGCGCATGGTGACCGAGGTGTTCAAGACCCAGCTGGACGTGGACCT
GGGCGAGTTCCCGATCATGACGTACCAGGACGCGGCGTTCCGCTTCGGCTCCGACAAGCCCGACCTGCGCGTGAAGCTCG
AGTTCACCGAACTGACCGAGGTCATGAAGGACGTGGACTTCAAGGTGTTCTCCGGCGCGGCCAACATGCAGGGCGGCCGG
GTGGTGGCCCTGCGCGTGCCTGGCGGCGCGCGCGAGAACGGCGGCCTGTCGCGTGGCGAGATCGATGCGTACACCGAGTT
CGTGAAGATCTACGGCGCCAAGGGGCTGGCCTACATCAAGGTCAACGAACTGGCGAAGGGCCGCGACGGCCTGCAGTCGC
CCATCGTGAAGAACATCCACGACGCGGCCATCGCCGAGATCCTGAAGCGCACCGGCGCGCAGGACGGTGACCTGCTGTTC
TTCGGCGCCGACAAGACCAAGGTGGTCAACGACGCGATCGGCGCGCTGCGCCTGAAGATCGGTCACAGCGAGTTCGGCAG
GAAGAACGGCCTGTTCGAGAACGTCTGGGCGCCCCTCTGGGTGGTGGACTTCCCGATGTTCGAGTACGACGAGGACGACG
CGCGCTGGGTGGCCGTGCACCATCCCTTCACGAGCCCCAAGGATGGCCATGAAGACCTGATGGATACCGACCCGGGCAAG
TGCCTCGCCAAGGCCTACGACATGGTGCTGAACGGCTGGGAGCTGGGCGGCGGCTCGGTGCGGATCCACCGTGCCGAGGT
GCAGAGCAAGGTGTTCGCGGCCCTCAAGCTCACGCCCGAGGACGCCCGCGCCAAGTTCGGCTACCTGCTGGACGCGCTGC
AGTACGGCGCGCCTCCGCATGGCGGCCTCGCCTTCGGCCTGGACCGCCTCATCACGCTGATGACGGGCGCCGAGTCGATC
CGCGACGTGATCGCCTTTCCCAAGACCCAGCGCGCGCAGGACCTGCTCACGCAGGCCCCGAGCCCGGTGGACGAAAAGCA
GCTGCGCGAACTGCACATCCGCCTGCGCAACCCGGACGCCGCCAAGGCCGCCTGA

Upstream 100 bases:

>100_bases
ACCGATTCCAAGTAACCCTTTCCCTTCGGCGCGCCCCGCGCGGGCGCCTGATACCTCCCTCCAGTTTGGGCGCCCCCCGC
GGGCGCAGGAAGAAGCACCC

Downstream 100 bases:

>100_bases
CCGGAGGCCGGCAACGGTTGACCCGGCATGGCAGGGGCCTCGCGGCTTCTGCCATTTTTTTAGCGAGGAACCTGTCACAT
GCGGACTTTCGCGAAGGGCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MAMRSHYCGLVTEALMGQTVTLAGWVNRRRDHGGVIFIDLRDREGNVQVVCDPDRADMFKTAEGVRNEFCVQVKGLVRAR
PEGTTNDSLKSGRIEVLCHELNVLNPSVTPPFQMDDENLSETTRLTHRVLDLRRPYMQRNLMLRYKTAIQVRNFLDKEGF
IDIETPMLGKSTPEGARDYLVPSRVHDGQFFALPQSPQLYKQMLMVAGYDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLNEEEIRAIFQRMVTEVFKTQLDVDLGEFPIMTYQDAAFRFGSDKPDLRVKLEFTELTEVMKDVDFKVFSGAANMQGGR
VVALRVPGGARENGGLSRGEIDAYTEFVKIYGAKGLAYIKVNELAKGRDGLQSPIVKNIHDAAIAEILKRTGAQDGDLLF
FGADKTKVVNDAIGALRLKIGHSEFGRKNGLFENVWAPLWVVDFPMFEYDEDDARWVAVHHPFTSPKDGHEDLMDTDPGK
CLAKAYDMVLNGWELGGGSVRIHRAEVQSKVFAALKLTPEDARAKFGYLLDALQYGAPPHGGLAFGLDRLITLMTGAESI
RDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRNPDAAKAA

Sequences:

>Translated_604_residues
MAMRSHYCGLVTEALMGQTVTLAGWVNRRRDHGGVIFIDLRDREGNVQVVCDPDRADMFKTAEGVRNEFCVQVKGLVRAR
PEGTTNDSLKSGRIEVLCHELNVLNPSVTPPFQMDDENLSETTRLTHRVLDLRRPYMQRNLMLRYKTAIQVRNFLDKEGF
IDIETPMLGKSTPEGARDYLVPSRVHDGQFFALPQSPQLYKQMLMVAGYDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLNEEEIRAIFQRMVTEVFKTQLDVDLGEFPIMTYQDAAFRFGSDKPDLRVKLEFTELTEVMKDVDFKVFSGAANMQGGR
VVALRVPGGARENGGLSRGEIDAYTEFVKIYGAKGLAYIKVNELAKGRDGLQSPIVKNIHDAAIAEILKRTGAQDGDLLF
FGADKTKVVNDAIGALRLKIGHSEFGRKNGLFENVWAPLWVVDFPMFEYDEDDARWVAVHHPFTSPKDGHEDLMDTDPGK
CLAKAYDMVLNGWELGGGSVRIHRAEVQSKVFAALKLTPEDARAKFGYLLDALQYGAPPHGGLAFGLDRLITLMTGAESI
RDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRNPDAAKAA
>Mature_603_residues
AMRSHYCGLVTEALMGQTVTLAGWVNRRRDHGGVIFIDLRDREGNVQVVCDPDRADMFKTAEGVRNEFCVQVKGLVRARP
EGTTNDSLKSGRIEVLCHELNVLNPSVTPPFQMDDENLSETTRLTHRVLDLRRPYMQRNLMLRYKTAIQVRNFLDKEGFI
DIETPMLGKSTPEGARDYLVPSRVHDGQFFALPQSPQLYKQMLMVAGYDRYYQITKCFRDEDLRADRQPEFTQIDCETSF
LNEEEIRAIFQRMVTEVFKTQLDVDLGEFPIMTYQDAAFRFGSDKPDLRVKLEFTELTEVMKDVDFKVFSGAANMQGGRV
VALRVPGGARENGGLSRGEIDAYTEFVKIYGAKGLAYIKVNELAKGRDGLQSPIVKNIHDAAIAEILKRTGAQDGDLLFF
GADKTKVVNDAIGALRLKIGHSEFGRKNGLFENVWAPLWVVDFPMFEYDEDDARWVAVHHPFTSPKDGHEDLMDTDPGKC
LAKAYDMVLNGWELGGGSVRIHRAEVQSKVFAALKLTPEDARAKFGYLLDALQYGAPPHGGLAFGLDRLITLMTGAESIR
DVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRNPDAAKAA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=614, Percent_Identity=37.9478827361564, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=248, Percent_Identity=28.2258064516129, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI194272210, Length=248, Percent_Identity=28.2258064516129, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI45439306, Length=310, Percent_Identity=28.0645161290323, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=57.3355817875211, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=273, Percent_Identity=26.007326007326, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789256, Length=269, Percent_Identity=27.1375464684015, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=617, Percent_Identity=32.7390599675851, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71994340, Length=263, Percent_Identity=24.3346007604563, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=263, Percent_Identity=24.3346007604563, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=263, Percent_Identity=24.3346007604563, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=266, Percent_Identity=27.4436090225564, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=643, Percent_Identity=34.6811819595645, Blast_Score=299, Evalue=8e-82,
Organism=Saccharomyces cerevisiae, GI6323011, Length=242, Percent_Identity=28.099173553719, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=268, Percent_Identity=26.4925373134328, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=28, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324256, Length=174, Percent_Identity=27.0114942528736, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24584738, Length=607, Percent_Identity=35.2553542009885, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI17136276, Length=288, Percent_Identity=28.8194444444444, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640849, Length=252, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640851, Length=252, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_ACIAC (A1TT99)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971961.1
- ProteinModelPortal:   A1TT99
- SMR:   A1TT99
- STRING:   A1TT99
- GeneID:   4665942
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3637
- NMPDR:   fig|397945.5.peg.3103
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   A1TT99
- ProtClustDB:   PRK00476
- BioCyc:   AAVE397945:AAVE_3637-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67975; Mature: 67844

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMRSHYCGLVTEALMGQTVTLAGWVNRRRDHGGVIFIDLRDREGNVQVVCDPDRADMFK
CCCCHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHH
TAEGVRNEFCVQVKGLVRARPEGTTNDSLKSGRIEVLCHELNVLNPSVTPPFQMDDENLS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCHH
ETTRLTHRVLDLRRPYMQRNLMLRYKTAIQVRNFLDKEGFIDIETPMLGKSTPEGARDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVHDGQFFALPQSPQLYKQMLMVAGYDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLNEEEIRAIFQRMVTEVFKTQLDVDLGEFPIMTYQDAAFRFGSDKPDLRVKLEFTELTE
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCEEEEEEHHHHHH
VMKDVDFKVFSGAANMQGGRVVALRVPGGARENGGLSRGEIDAYTEFVKIYGAKGLAYIK
HHHHCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
VNELAKGRDGLQSPIVKNIHDAAIAEILKRTGAQDGDLLFFGADKTKVVNDAIGALRLKI
EHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCEEEEEE
GHSEFGRKNGLFENVWAPLWVVDFPMFEYDEDDARWVAVHHPFTSPKDGHEDLMDTDPGK
CCHHHCCCCCCHHHHCCCEEEEECCCEECCCCCCEEEEEECCCCCCCCCCHHHCCCCCHH
CLAKAYDMVLNGWELGGGSVRIHRAEVQSKVFAALKLTPEDARAKFGYLLDALQYGAPPH
HHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCC
GGLAFGLDRLITLMTGAESIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRNPDA
CCHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHEECCCCC
AKAA
CCCC
>Mature Secondary Structure 
AMRSHYCGLVTEALMGQTVTLAGWVNRRRDHGGVIFIDLRDREGNVQVVCDPDRADMFK
CCCHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHH
TAEGVRNEFCVQVKGLVRARPEGTTNDSLKSGRIEVLCHELNVLNPSVTPPFQMDDENLS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCHH
ETTRLTHRVLDLRRPYMQRNLMLRYKTAIQVRNFLDKEGFIDIETPMLGKSTPEGARDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVHDGQFFALPQSPQLYKQMLMVAGYDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLNEEEIRAIFQRMVTEVFKTQLDVDLGEFPIMTYQDAAFRFGSDKPDLRVKLEFTELTE
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCEEEEEEHHHHHH
VMKDVDFKVFSGAANMQGGRVVALRVPGGARENGGLSRGEIDAYTEFVKIYGAKGLAYIK
HHHHCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
VNELAKGRDGLQSPIVKNIHDAAIAEILKRTGAQDGDLLFFGADKTKVVNDAIGALRLKI
EHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCEEEEEE
GHSEFGRKNGLFENVWAPLWVVDFPMFEYDEDDARWVAVHHPFTSPKDGHEDLMDTDPGK
CCHHHCCCCCCHHHHCCCEEEEECCCEECCCCCCEEEEEECCCCCCCCCCHHHCCCCCHH
CLAKAYDMVLNGWELGGGSVRIHRAEVQSKVFAALKLTPEDARAKFGYLLDALQYGAPPH
HHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCC
GGLAFGLDRLITLMTGAESIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRNPDA
CCHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHEECCCCC
AKAA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA