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The map label for this gene is ppnK

Identifier: 120612248

GI number: 120612248

Start: 3972822

End: 3973718

Strand: Direct

Name: ppnK

Synonym: Aave_3601

Alternate gene names: 120612248

Gene position: 3972822-3973718 (Clockwise)

Preceding gene: 120612239

Following gene: 120612249

Centisome position: 74.22

GC content: 67.56

Gene sequence:

>897_bases
ATGAAGCTCAGGTTCCAACGCGTGGCGCTCGTCGGCAAGTACCAGGCGCCCACTTCTGCAGGCATGTCCGACAGCTCGCG
CGACGCGCTGGACGGCATCGCCAGATTCCTGGCCAACGAGGGGTGCGAGGTCGCCCTCGAGGCGGATACCGCCGCGAACA
CGGGGTTCACCGACTACCCGGCGCTTTCCGTGGAGCGCATCGGGCTGGACTGCGATCTCTGCCTCGTGGTGGGCGGCGAC
GGCACGATGCTGGGCGTCGGCCGGCAGCTCGCCCAGTACCGCACGCCGCTGATCGGCATCAACCAGGGCCGGCTCGGCTT
CATCACCGACATTCCCCTGGGCGAGTACCCGACGGTCCTCAAACCCATGCTGCGCGGCGAGTACGAGGAAGACCTGCGCC
CCCTCATGCGGGCCCGGGTGATGCGCCAGGGCCAGTGCGTGTTCGAGGCACTGGCGATGAACGACGTGGTGGTCAACCGC
GGGTCCACCTCCGGGATGGTGGAACTGCGCGTGGAAGTGGGCGGCCATTTCGTCTCCAACCAGCGCGCAGACGGCCTCAT
CATCGCTTCGCCCACGGGCTCCACCGCCTATGCCCTGTCCGCAGGCGGCCCGATGCTGCACCCGACGATTCCCGGCTGGG
TGCTGGCGCCGATCGCGCCGCATACGCTGTCGAACCGGCCCATCGTACTGTCGGACAGCATGGAAGTGGCCGTGGAAGTC
GTCAGCGGGCGCGATGTCAGCGCGAATTTCGACATGCAGTCCCTCGCGTCCCTGCAGCACGGCGACCGCATCCTGGTACA
GCGCTCGGACTACCGGGCGCGCTTTCTCCATCCGCGCGGCTGGAACTACTTCGCCACGCTGCGCAAGAAGCTCCGCTGGA
ACGAAGGAGGCTACTGA

Upstream 100 bases:

>100_bases
CTGCTAGAAGCGCAACGCGAGGCAGAGAAAAAGCCGGCCGTACAACGCATGCTGGACCGAATGCGGAGCCTCAGATTACT
TTAATGATCTAATTTCCGCG

Downstream 100 bases:

>100_bases
GCATGGCGTTGAAGCGGATTGCGCTGCGTGATTTCGTGATCGTCGAGTCCCTCGAGCTGGACCTCCATTCCGGATTCACC
GTCCTGACCGGCGAAACCGG

Product: NAD(+)/NADH kinase family protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKLRFQRVALVGKYQAPTSAGMSDSSRDALDGIARFLANEGCEVALEADTAANTGFTDYPALSVERIGLDCDLCLVVGGD
GTMLGVGRQLAQYRTPLIGINQGRLGFITDIPLGEYPTVLKPMLRGEYEEDLRPLMRARVMRQGQCVFEALAMNDVVVNR
GSTSGMVELRVEVGGHFVSNQRADGLIIASPTGSTAYALSAGGPMLHPTIPGWVLAPIAPHTLSNRPIVLSDSMEVAVEV
VSGRDVSANFDMQSLASLQHGDRILVQRSDYRARFLHPRGWNYFATLRKKLRWNEGGY

Sequences:

>Translated_298_residues
MKLRFQRVALVGKYQAPTSAGMSDSSRDALDGIARFLANEGCEVALEADTAANTGFTDYPALSVERIGLDCDLCLVVGGD
GTMLGVGRQLAQYRTPLIGINQGRLGFITDIPLGEYPTVLKPMLRGEYEEDLRPLMRARVMRQGQCVFEALAMNDVVVNR
GSTSGMVELRVEVGGHFVSNQRADGLIIASPTGSTAYALSAGGPMLHPTIPGWVLAPIAPHTLSNRPIVLSDSMEVAVEV
VSGRDVSANFDMQSLASLQHGDRILVQRSDYRARFLHPRGWNYFATLRKKLRWNEGGY
>Mature_298_residues
MKLRFQRVALVGKYQAPTSAGMSDSSRDALDGIARFLANEGCEVALEADTAANTGFTDYPALSVERIGLDCDLCLVVGGD
GTMLGVGRQLAQYRTPLIGINQGRLGFITDIPLGEYPTVLKPMLRGEYEEDLRPLMRARVMRQGQCVFEALAMNDVVVNR
GSTSGMVELRVEVGGHFVSNQRADGLIIASPTGSTAYALSAGGPMLHPTIPGWVLAPIAPHTLSNRPIVLSDSMEVAVEV
VSGRDVSANFDMQSLASLQHGDRILVQRSDYRARFLHPRGWNYFATLRKKLRWNEGGY

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=253, Percent_Identity=28.0632411067194, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788968, Length=296, Percent_Identity=37.1621621621622, Blast_Score=200, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6320794, Length=241, Percent_Identity=31.9502074688797, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6322509, Length=282, Percent_Identity=29.7872340425532, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325068, Length=172, Percent_Identity=31.3953488372093, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28573828, Length=245, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161077047, Length=245, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28573826, Length=245, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28573830, Length=245, Percent_Identity=31.4285714285714, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28573832, Length=245, Percent_Identity=31.4285714285714, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI20129957, Length=270, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653422, Length=270, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653424, Length=270, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281363321, Length=270, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281363323, Length=270, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_ACIAC (A1TT64)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971926.1
- ProteinModelPortal:   A1TT64
- STRING:   A1TT64
- GeneID:   4666930
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3601
- NMPDR:   fig|397945.5.peg.3070
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   LNEMVLH
- PhylomeDB:   A1TT64
- ProtClustDB:   PRK02155
- BioCyc:   AAVE397945:AAVE_3601-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32444; Mature: 32444

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRFQRVALVGKYQAPTSAGMSDSSRDALDGIARFLANEGCEVALEADTAANTGFTDYP
CCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
ALSVERIGLDCDLCLVVGGDGTMLGVGRQLAQYRTPLIGINQGRLGFITDIPLGEYPTVL
CCEEEEECCCCEEEEEECCCCCEEHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCHHH
KPMLRGEYEEDLRPLMRARVMRQGQCVFEALAMNDVVVNRGSTSGMVELRVEVGGHFVSN
HHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCEEEEEEEECCEEECC
QRADGLIIASPTGSTAYALSAGGPMLHPTIPGWVLAPIAPHTLSNRPIVLSDSMEVAVEV
CCCCEEEEECCCCCCEEEEECCCCEECCCCCCEEECCCCCCCCCCCCEEEECCCEEEEEE
VSGRDVSANFDMQSLASLQHGDRILVQRSDYRARFLHPRGWNYFATLRKKLRWNEGGY
ECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLRFQRVALVGKYQAPTSAGMSDSSRDALDGIARFLANEGCEVALEADTAANTGFTDYP
CCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
ALSVERIGLDCDLCLVVGGDGTMLGVGRQLAQYRTPLIGINQGRLGFITDIPLGEYPTVL
CCEEEEECCCCEEEEEECCCCCEEHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCHHH
KPMLRGEYEEDLRPLMRARVMRQGQCVFEALAMNDVVVNRGSTSGMVELRVEVGGHFVSN
HHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCEEEEEEEECCEEECC
QRADGLIIASPTGSTAYALSAGGPMLHPTIPGWVLAPIAPHTLSNRPIVLSDSMEVAVEV
CCCCEEEEECCCCCCEEEEECCCCEECCCCCCEEECCCCCCCCCCCCEEEECCCEEEEEE
VSGRDVSANFDMQSLASLQHGDRILVQRSDYRARFLHPRGWNYFATLRKKLRWNEGGY
ECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA