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The map label for this gene is greB [H]

Identifier: 120612233

GI number: 120612233

Start: 3957678

End: 3958244

Strand: Reverse

Name: greB [H]

Synonym: Aave_3586

Alternate gene names: 120612233

Gene position: 3958244-3957678 (Counterclockwise)

Preceding gene: 120612234

Following gene: 120612228

Centisome position: 73.95

GC content: 66.67

Gene sequence:

>567_bases
ATGAGCAAGGCCTTCACCAAGGAAACGGACAACCCCCCCGACGACGATGCGGAGTTCCCATCCGCGCCCCTTCCGGCGGG
CAGCAAGAACTACATCACGCCGGCAGGGTATGCGCGGCTGCGGTCGGAACTGCTCGACCTCATGGACAACGAGCGGCCCA
AGGTGGTCGAGGTCGTCCATTGGGCCGCGAGCAACGGTGACCGTTCCGAGAATGGCGACTACCTCTATGGCAAGAAGCGC
CTGCGCGAGATCGACCGGCGCATCCGTTTCCTGACCAAGCGCCTGGACATCGCCGAGGTGGTCGATCCTTCCCTGCACCA
CGGCAGCGACCAGGTGTTCTTCGGCGCTACGGTCACCTACGTGGACGACCTGGACGTGGAGCGGACGGTGACCATCCTGG
GAATCGACGAGGTGGACAGCGGCCAGTCCCAGGTGAGCTGGGTGTCGCCGGTGGCCCGCGCCCTGCTGAAAGCGCGCGAG
GGCGACGAGGTCAGGCTGGGTACGCCCGGCGGACTGCGCACGCTGGAGATCCTGCGGGTGGAGTACCCGGCGCGAGCGCC
TTCCTGA

Upstream 100 bases:

>100_bases
GCACCAAATGGTAAGCCTCGTGATGTCAAAGTCACGAGGCTTTTTCCATTTTCTGCCAGCTGCCGCTGGTGGTCGTCATC
ACCTGGAGCCGACCGATCCC

Downstream 100 bases:

>100_bases
CACCCTTCCTGCGCCCATTGGCGGCGCGAAGGGATGTGGGGGAATGCCCTCCTTTGGAAAGGGCGGGCGCAGCCCGCGTG
CGGCTAGGGCGACGGCAGGA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSKAFTKETDNPPDDDAEFPSAPLPAGSKNYITPAGYARLRSELLDLMDNERPKVVEVVHWAASNGDRSENGDYLYGKKR
LREIDRRIRFLTKRLDIAEVVDPSLHHGSDQVFFGATVTYVDDLDVERTVTILGIDEVDSGQSQVSWVSPVARALLKARE
GDEVRLGTPGGLRTLEILRVEYPARAPS

Sequences:

>Translated_188_residues
MSKAFTKETDNPPDDDAEFPSAPLPAGSKNYITPAGYARLRSELLDLMDNERPKVVEVVHWAASNGDRSENGDYLYGKKR
LREIDRRIRFLTKRLDIAEVVDPSLHHGSDQVFFGATVTYVDDLDVERTVTILGIDEVDSGQSQVSWVSPVARALLKARE
GDEVRLGTPGGLRTLEILRVEYPARAPS
>Mature_187_residues
SKAFTKETDNPPDDDAEFPSAPLPAGSKNYITPAGYARLRSELLDLMDNERPKVVEVVHWAASNGDRSENGDYLYGKKRL
REIDRRIRFLTKRLDIAEVVDPSLHHGSDQVFFGATVTYVDDLDVERTVTILGIDEVDSGQSQVSWVSPVARALLKAREG
DEVRLGTPGGLRTLEILRVEYPARAPS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=47.0198675496689, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=36.1842105263158, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20925; Mature: 20793

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAFTKETDNPPDDDAEFPSAPLPAGSKNYITPAGYARLRSELLDLMDNERPKVVEVVH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
WAASNGDRSENGDYLYGKKRLREIDRRIRFLTKRLDIAEVVDPSLHHGSDQVFFGATVTY
HHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCEEEEEEEEE
VDDLDVERTVTILGIDEVDSGQSQVSWVSPVARALLKAREGDEVRLGTPGGLRTLEILRV
ECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEE
EYPARAPS
ECCCCCCC
>Mature Secondary Structure 
SKAFTKETDNPPDDDAEFPSAPLPAGSKNYITPAGYARLRSELLDLMDNERPKVVEVVH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
WAASNGDRSENGDYLYGKKRLREIDRRIRFLTKRLDIAEVVDPSLHHGSDQVFFGATVTY
HHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCEEEEEEEEE
VDDLDVERTVTILGIDEVDSGQSQVSWVSPVARALLKAREGDEVRLGTPGGLRTLEILRV
ECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEE
EYPARAPS
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]