Definition Acidovorax citrulli AAC00-1 chromosome, complete genome.
Accession NC_008752
Length 5,352,772

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The map label for this gene is sigK [H]

Identifier: 120612217

GI number: 120612217

Start: 3941359

End: 3942024

Strand: Reverse

Name: sigK [H]

Synonym: Aave_3570

Alternate gene names: 120612217

Gene position: 3942024-3941359 (Counterclockwise)

Preceding gene: 120612221

Following gene: 120612216

Centisome position: 73.64

GC content: 68.32

Gene sequence:

>666_bases
ATGACCATCGTCCGCAAGCCCCTGGCAGAGCAGCCTCCCGCGAGGACCGCTTTCGCTCTGCCTCCCTACCTGTCGATTGT
GTCGAAGTATCACACCGAAGGACTGCCTTCATCGCCTTCGTCGGAAGCGTGGATCGCCCTGGTGCGCGCCGTGGCCGACC
ACGGCGACCGGCAGGCCTTCGCGGCGCTGTTCAAGCATTTCGCCCCGCGGGTGAAGGCCTACCTGATGCGCCTCGGCCTG
GCGGAGGGCGTGGCGGAAGACCTCACGCAGGAAGCGATGGTCAATCTCTGGCGCAGGGCGGGCACCTTCGACCCGGCGCA
TGCGGGTGTCTCCACGTGGGTGTTCACCATCGCGCGGAACCTGCGCATCGACCATCTGCGCCGCAAGTCCAACCAGTTGT
CCGAAAGCGACGACGGGCTCGACATGGCCCTGGACGACGATGGCCCCGAGGAGCTGGCCTACGCCGCCCAGTGCGAAAAC
GACGTGCGGCGGGCGATCGAGTCGCTCGCCCCCGAACAGGCACAGGTGCTGCGCCGCTCCTTCTTCGAGGACCAGCCCCA
CGCCCGGATCGCCGAGGATCTCGGCATCCCGCTGGGGACCGTGAAATCCCGCGTGCGCCTGGCCGTACGCCACCTGCGCC
GCCTGCTCGGCGCCCATGCGCCATGA

Upstream 100 bases:

>100_bases
GTCCTCGGATGCTGCCGTGCTGTACGCATCTGCCAGGCTCTTGGATTGCGATGCCGCGGGGCGTGATCTAATCCACCCAT
CGCTGCGTAACGGCAGCACC

Downstream 100 bases:

>100_bases
CGATCCATCATCATCCCGGAGATGACCTGCTCCTCGACCTGGCCGCGGGGCGGCTGCCGCCGGGGCCGGCGCTGCTGGCT
TCGGTGCATGCCGAGATGTG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTIVRKPLAEQPPARTAFALPPYLSIVSKYHTEGLPSSPSSEAWIALVRAVADHGDRQAFAALFKHFAPRVKAYLMRLGL
AEGVAEDLTQEAMVNLWRRAGTFDPAHAGVSTWVFTIARNLRIDHLRRKSNQLSESDDGLDMALDDDGPEELAYAAQCEN
DVRRAIESLAPEQAQVLRRSFFEDQPHARIAEDLGIPLGTVKSRVRLAVRHLRRLLGAHAP

Sequences:

>Translated_221_residues
MTIVRKPLAEQPPARTAFALPPYLSIVSKYHTEGLPSSPSSEAWIALVRAVADHGDRQAFAALFKHFAPRVKAYLMRLGL
AEGVAEDLTQEAMVNLWRRAGTFDPAHAGVSTWVFTIARNLRIDHLRRKSNQLSESDDGLDMALDDDGPEELAYAAQCEN
DVRRAIESLAPEQAQVLRRSFFEDQPHARIAEDLGIPLGTVKSRVRLAVRHLRRLLGAHAP
>Mature_220_residues
TIVRKPLAEQPPARTAFALPPYLSIVSKYHTEGLPSSPSSEAWIALVRAVADHGDRQAFAALFKHFAPRVKAYLMRLGLA
EGVAEDLTQEAMVNLWRRAGTFDPAHAGVSTWVFTIARNLRIDHLRRKSNQLSESDDGLDMALDDDGPEELAYAAQCEND
VRRAIESLAPEQAQVLRRSFFEDQPHARIAEDLGIPLGTVKSRVRLAVRHLRRLLGAHAP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 24562; Mature: 24431

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVRKPLAEQPPARTAFALPPYLSIVSKYHTEGLPSSPSSEAWIALVRAVADHGDRQAF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHH
AALFKHFAPRVKAYLMRLGLAEGVAEDLTQEAMVNLWRRAGTFDPAHAGVSTWVFTIARN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LRIDHLRRKSNQLSESDDGLDMALDDDGPEELAYAAQCENDVRRAIESLAPEQAQVLRRS
HHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FFEDQPHARIAEDLGIPLGTVKSRVRLAVRHLRRLLGAHAP
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIVRKPLAEQPPARTAFALPPYLSIVSKYHTEGLPSSPSSEAWIALVRAVADHGDRQAF
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHH
AALFKHFAPRVKAYLMRLGLAEGVAEDLTQEAMVNLWRRAGTFDPAHAGVSTWVFTIARN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LRIDHLRRKSNQLSESDDGLDMALDDDGPEELAYAAQCENDVRRAIESLAPEQAQVLRRS
HHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FFEDQPHARIAEDLGIPLGTVKSRVRLAVRHLRRLLGAHAP
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]