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The map label for this gene is truB [H]

Identifier: 120612035

GI number: 120612035

Start: 3736001

End: 3736999

Strand: Reverse

Name: truB [H]

Synonym: Aave_3381

Alternate gene names: 120612035

Gene position: 3736999-3736001 (Counterclockwise)

Preceding gene: 120612036

Following gene: 120612034

Centisome position: 69.81

GC content: 71.07

Gene sequence:

>999_bases
ATGACCGCACGCGCTCCCCGCATCCGGGTGCAGCGGCGCCCGGTGCACGGAGTGCTTCTGCTCGACAAGCCCCTTGGCCT
TTCCAGCAACGATGCGTTGCAGAAGGCCAAGTGGCTGCTGCGCGCCGAAAAGGCCGGCCACACCGGCACGCTCGATCCTC
TGGCCTCTGGCGTGCTGCCGCTGTGCTTCGGCGCGGCCACCAAGTTCAGCCAGCTGCAGCTCGATGCGCCCAAGACCTAC
GAGGCCGTCGCCCGGCTCGGCGTGACCACGACCACGGGCGACGCGGAAGGCGACGTGGTGGAGCGCCGGGAGGTGGATGC
CTCGCAGCTCGCCGCCGCGCGCCTGGCCGAGGTGCAGCGGCAGTTCACCGGCCCCATCCGGCAGTTGCCGCCGATGCACA
GCGCCCTCAAGAAGGATGGCAAGGCGCTGTACGAATACGCCCGCGCGGGCATCGCCGTCGAGCGCGAGGCGCGCGACGTG
GTGATCCATGCGCTGGAACTGTCGCTGGCAGCGGACCAGGGCGGCGAGCCTGCCGTGCGCATGGTCGTGACCTGCAGCAA
GGGCACCTACATCCGCACGCTGGGCGAGGACGTGGGCGCCGCACTGGGTTGCGGCGCGCACCTCGTCTTCCTGCGCCGTA
TCGACACCGGCGGGCTGGGCGTGGAGCGCTGCATCACGCTCGCCGCGCTCGAAGCGCTGGACGAGGCCGGGCGCATGGCC
TGCGTGCAGCCGGTGGAAACGCTACTCGCGGGGCATGCATCTGTCACGCTGGACCATGACAATACGGCGCGATTCCTCTC
CGGCATGCGCCGCCGCGGCCCCTGGCCCGACGCGCCCGCCGTGGCGGTGTTCGGCCAGCATCCCCGCGCCCTGCTGGGCG
TCGGCCGGGTGGCGGCCGGCGAACTCATCCCCGAGCGCCTCCTGAGCCCCCAGGAGATCGCACAGATTCTGGAAAGCACG
CCGCGGGCAGAGCGCGGCACATTGGAACCCACTCTATGA

Upstream 100 bases:

>100_bases
CGCTGCACTTCATGTTCGACCGCACCACCGAACGTGCTTCGGACATGAATGCACTGATCGCGCGCGCCGTGGCTTCCCGT
TCCAAAGACGACGACGAAGC

Downstream 100 bases:

>100_bases
GCAAACAAATCCGCAACATCGCCATCATCGCGCACGTTGACCATGGCAAGACCACCATGGTGGACCAGCTGCTGCGCCAG
TCCGGCACCTTCGCCGACCA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTARAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKAKWLLRAEKAGHTGTLDPLASGVLPLCFGAATKFSQLQLDAPKTY
EAVARLGVTTTTGDAEGDVVERREVDASQLAAARLAEVQRQFTGPIRQLPPMHSALKKDGKALYEYARAGIAVEREARDV
VIHALELSLAADQGGEPAVRMVVTCSKGTYIRTLGEDVGAALGCGAHLVFLRRIDTGGLGVERCITLAALEALDEAGRMA
CVQPVETLLAGHASVTLDHDNTARFLSGMRRRGPWPDAPAVAVFGQHPRALLGVGRVAAGELIPERLLSPQEIAQILEST
PRAERGTLEPTL

Sequences:

>Translated_332_residues
MTARAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKAKWLLRAEKAGHTGTLDPLASGVLPLCFGAATKFSQLQLDAPKTY
EAVARLGVTTTTGDAEGDVVERREVDASQLAAARLAEVQRQFTGPIRQLPPMHSALKKDGKALYEYARAGIAVEREARDV
VIHALELSLAADQGGEPAVRMVVTCSKGTYIRTLGEDVGAALGCGAHLVFLRRIDTGGLGVERCITLAALEALDEAGRMA
CVQPVETLLAGHASVTLDHDNTARFLSGMRRRGPWPDAPAVAVFGQHPRALLGVGRVAAGELIPERLLSPQEIAQILEST
PRAERGTLEPTL
>Mature_331_residues
TARAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKAKWLLRAEKAGHTGTLDPLASGVLPLCFGAATKFSQLQLDAPKTYE
AVARLGVTTTTGDAEGDVVERREVDASQLAAARLAEVQRQFTGPIRQLPPMHSALKKDGKALYEYARAGIAVEREARDVV
IHALELSLAADQGGEPAVRMVVTCSKGTYIRTLGEDVGAALGCGAHLVFLRRIDTGGLGVERCITLAALEALDEAGRMAC
VQPVETLLAGHASVTLDHDNTARFLSGMRRRGPWPDAPAVAVFGQHPRALLGVGRVAAGELIPERLLSPQEIAQILESTP
RAERGTLEPTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=226, Percent_Identity=33.6283185840708, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI215599015, Length=250, Percent_Identity=28, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4503337, Length=250, Percent_Identity=28, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI2367200, Length=242, Percent_Identity=50.4132231404959, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17553978, Length=253, Percent_Identity=28.4584980237154, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=44.8818897637795, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=250, Percent_Identity=30.8, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281364189, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364187, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364185, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364183, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62471759, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17975520, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35436; Mature: 35305

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKAKWLLRAEKAGHTGTLDPLASGVLP
CCCCCCCCEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LCFGAATKFSQLQLDAPKTYEAVARLGVTTTTGDAEGDVVERREVDASQLAAARLAEVQR
HHHHHHCCHHHEECCCCHHHHHHHHHCCEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHH
QFTGPIRQLPPMHSALKKDGKALYEYARAGIAVEREARDVVIHALELSLAADQGGEPAVR
HHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEE
MVVTCSKGTYIRTLGEDVGAALGCGAHLVFLRRIDTGGLGVERCITLAALEALDEAGRMA
EEEEECCCCEEEHHHHHHHHHHCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHH
CVQPVETLLAGHASVTLDHDNTARFLSGMRRRGPWPDAPAVAVFGQHPRALLGVGRVAAG
HHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCHHHHH
ELIPERLLSPQEIAQILESTPRAERGTLEPTL
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TARAPRIRVQRRPVHGVLLLDKPLGLSSNDALQKAKWLLRAEKAGHTGTLDPLASGVLP
CCCCCCCEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LCFGAATKFSQLQLDAPKTYEAVARLGVTTTTGDAEGDVVERREVDASQLAAARLAEVQR
HHHHHHCCHHHEECCCCHHHHHHHHHCCEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHH
QFTGPIRQLPPMHSALKKDGKALYEYARAGIAVEREARDVVIHALELSLAADQGGEPAVR
HHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEE
MVVTCSKGTYIRTLGEDVGAALGCGAHLVFLRRIDTGGLGVERCITLAALEALDEAGRMA
EEEEECCCCEEEHHHHHHHHHHCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHH
CVQPVETLLAGHASVTLDHDNTARFLSGMRRRGPWPDAPAVAVFGQHPRALLGVGRVAAG
HHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCHHHHH
ELIPERLLSPQEIAQILESTPRAERGTLEPTL
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA