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The map label for this gene is pinE [H]

Identifier: 120611368

GI number: 120611368

Start: 2968324

End: 2969247

Strand: Reverse

Name: pinE [H]

Synonym: Aave_2702

Alternate gene names: 120611368

Gene position: 2969247-2968324 (Counterclockwise)

Preceding gene: 120611375

Following gene: 120611358

Centisome position: 55.47

GC content: 63.74

Gene sequence:

>924_bases
ATGAAAATCGGCTACGCTCGCGTCTCCACGCGAGAACAGAACCCGGCCTTGCAGGTGGACGCTCTCAAGGCGGCCGGTTG
CGAGCGCATCTATCAGGATGTGGCGAGCGGCGCGAAGACCGCACGTCCGGCGCTCGATGAGCTACTGGGTCAGTTGCGCG
CCGGCGACGTGCTGGTGATCTGGAAGCTCGACCGCATGGGGCGCTCGCTCAAGCACCTGGTCGAGCTGGTCGGCAACCTG
ATGGAGCGCAAGGTTGGACTCCTCAGTCTGAACGATCCCATCGACACCACCAGCGCCCAGGGGCGGTTTGTGTTCAACCT
GTTCGCTACGCTGGCCGAGTTCGAGCGCGAACTGATCCGCGAACGCACCCAGGCCGGGCTGACGGCCGCGCGGGCACGCG
GTCGGGTCGGCGGGCGACCCACAGGACTGTCGCCGCAGGCCGAAGCGACGGCGCTGGCGGCAGAGACCCTGTACCGCGAG
CGGAAACTGTCGGTCGCCGCCATCGCACAGAAGCTGCACCTGTCCAAGAGCACGCTGTACAGCTACCTGCGACATCGCGG
TGTAGAGATCGGTCCGTACAAGAAAACGGAGCAGCAACCATCAAGCATGTCGGCCCTTGAAAGCGGCAATGACGCCCCGC
CGAAAGTCGCCACCATCCTGCTGACGCTGCGCATCGAGAACAACAGCAAGTTCGTGCGCGGCAAGAAGCGCACGATTGAG
CACGTCGAGAGCTTTTACCTCGACCAATACGACGCCACGCGACGACCGAACGGCGAATATGAGCTGAAGGTGCCCTATGA
CACCGACGAAGAACTGGATGAAGCTGTGAACGAATTGCTGACCGACATCGCCAGCGGTGCGGACGACCGGCACTGCTTCT
CGGAGAGCGACGCGCGCATGGAGGGCACCGACCGGCACTGGTGA

Upstream 100 bases:

>100_bases
GGGCATGACATGCCCGCAGGCCAACAAGTAGTCTATAAAAGTGGTTTGCATAATCAAAGTTTGATATTGTCTTAATCAAC
GAGTAAAACGGAATTTTCGG

Downstream 100 bases:

>100_bases
AGGCTTAACTGACTTCCCTGTTCCATTGCTCCCCGAACCCCAATCCGCCATGAGCGCCTGGCAGCCTGCTGTACCGTGTA
GACGTTGAAGCCCGACTGCA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKIGYARVSTREQNPALQVDALKAAGCERIYQDVASGAKTARPALDELLGQLRAGDVLVIWKLDRMGRSLKHLVELVGNL
MERKVGLLSLNDPIDTTSAQGRFVFNLFATLAEFERELIRERTQAGLTAARARGRVGGRPTGLSPQAEATALAAETLYRE
RKLSVAAIAQKLHLSKSTLYSYLRHRGVEIGPYKKTEQQPSSMSALESGNDAPPKVATILLTLRIENNSKFVRGKKRTIE
HVESFYLDQYDATRRPNGEYELKVPYDTDEELDEAVNELLTDIASGADDRHCFSESDARMEGTDRHW

Sequences:

>Translated_307_residues
MKIGYARVSTREQNPALQVDALKAAGCERIYQDVASGAKTARPALDELLGQLRAGDVLVIWKLDRMGRSLKHLVELVGNL
MERKVGLLSLNDPIDTTSAQGRFVFNLFATLAEFERELIRERTQAGLTAARARGRVGGRPTGLSPQAEATALAAETLYRE
RKLSVAAIAQKLHLSKSTLYSYLRHRGVEIGPYKKTEQQPSSMSALESGNDAPPKVATILLTLRIENNSKFVRGKKRTIE
HVESFYLDQYDATRRPNGEYELKVPYDTDEELDEAVNELLTDIASGADDRHCFSESDARMEGTDRHW
>Mature_307_residues
MKIGYARVSTREQNPALQVDALKAAGCERIYQDVASGAKTARPALDELLGQLRAGDVLVIWKLDRMGRSLKHLVELVGNL
MERKVGLLSLNDPIDTTSAQGRFVFNLFATLAEFERELIRERTQAGLTAARARGRVGGRPTGLSPQAEATALAAETLYRE
RKLSVAAIAQKLHLSKSTLYSYLRHRGVEIGPYKKTEQQPSSMSALESGNDAPPKVATILLTLRIENNSKFVRGKKRTIE
HVESFYLDQYDATRRPNGEYELKVPYDTDEELDEAVNELLTDIASGADDRHCFSESDARMEGTDRHW

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=182, Percent_Identity=51.6483516483517, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1787638, Length=185, Percent_Identity=32.4324324324324, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787827, Length=185, Percent_Identity=32.4324324324324, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 34218; Mature: 34218

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGYARVSTREQNPALQVDALKAAGCERIYQDVASGAKTARPALDELLGQLRAGDVLVI
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
WKLDRMGRSLKHLVELVGNLMERKVGLLSLNDPIDTTSAQGRFVFNLFATLAEFERELIR
EEHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERTQAGLTAARARGRVGGRPTGLSPQAEATALAAETLYRERKLSVAAIAQKLHLSKSTLY
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLRHRGVEIGPYKKTEQQPSSMSALESGNDAPPKVATILLTLRIENNSKFVRGKKRTIE
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHH
HVESFYLDQYDATRRPNGEYELKVPYDTDEELDEAVNELLTDIASGADDRHCFSESDARM
HHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
EGTDRHW
CCCCCCC
>Mature Secondary Structure
MKIGYARVSTREQNPALQVDALKAAGCERIYQDVASGAKTARPALDELLGQLRAGDVLVI
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
WKLDRMGRSLKHLVELVGNLMERKVGLLSLNDPIDTTSAQGRFVFNLFATLAEFERELIR
EEHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERTQAGLTAARARGRVGGRPTGLSPQAEATALAAETLYRERKLSVAAIAQKLHLSKSTLY
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLRHRGVEIGPYKKTEQQPSSMSALESGNDAPPKVATILLTLRIENNSKFVRGKKRTIE
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHH
HVESFYLDQYDATRRPNGEYELKVPYDTDEELDEAVNELLTDIASGADDRHCFSESDARM
HHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
EGTDRHW
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]