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The map label for this gene is ansA [H]

Identifier: 120611228

GI number: 120611228

Start: 2811533

End: 2812510

Strand: Direct

Name: ansA [H]

Synonym: Aave_2559

Alternate gene names: 120611228

Gene position: 2811533-2812510 (Clockwise)

Preceding gene: 120611225

Following gene: 120611236

Centisome position: 52.52

GC content: 73.31

Gene sequence:

>978_bases
ATGCAAGTGGTTGCAGGAAAAATCCTGGTGCTGGGCACCGGCGGAACCATCGCCGGTACGGCCGGAAACGCGAAAGACAA
CATCGGCTACACCGCCGCGCAGCTCGGCATCGACCAGTTGCTGGCAGCGGTGCCCGGCCTGGAGCGCGCCGCCGGCGGCG
CGCGGCTGGAAGCCGAACAGGTCGCGCAGATCGACAGCAAGGACATGGACGGGGCCGTATGGCGTGTGCTGGCCCTGCGG
TGCGCCGAAGCGCTGGCCGATCCCGAAATGCGGGCCCTCGTGGTCACGCATGGCACCGACACGCTCGAGGAAACCGCATG
GTTCCTCCACCGTGTGCTGGCGGGCGCGCGGGACAAGCCCGTGGTGCTCGTCTCGGCCATGCGTCCGGCCACCGCGCTGG
CGCCCGACGGCCCGCAGAACCTGCTCGACGCCGTCTCCGTGGCCGCCACGCCGGGCGCGCGCGGGGTCGTGGCCGTGGCC
GCCGGGGAAGTGCACGGGGCGCAGCGGGTACAGAAGGTGCATCCTTACCGGCTGAATGCCTTCGCGTCCGGCGAGGCGGG
GCCTCTGGGCTGGATGGAGGAGGGCCGCGTGCGGCTCGCCGCCGGCTGGCCCGCGCCGGACTGTTCGGCCGAGGCTGACG
CCGCGCTGAAGGCGCTGCGCAGCGGTGCGGAGTGGCCGCGCGTCGAGGTCGTGATGTCGCACGCGGGCGCGACCGGACGC
TTGGTGGATCTGCTGGTGGCCGACGGGGTGCACGGCCTGGCCGTGGCCGCCACCGGCAACGGCACGCTGCACCACCATCT
GCAGGCCGCGCTGGAGCGGGCACAGGCCGCTGGCGTGGCCGTGCGGGTGGCCACGCGGTGTTCCCTGGGCCAGCTGCTGC
CCCGTCCTGGCGACACGCTGCCCGTCGCGCAGGGGCTGAGCGTGGTGAAGACGCGGGTGGAACTGCTGCTGGAGCGGCTG
GCGCAGCCCCAGGCCTAG

Upstream 100 bases:

>100_bases
GCACTCCAGTGAAGGAAGATCGCGCCGGACGGGCGCTGGGCGGATCGGTTCACCTGTGGATCGATCCAGTCACTGTATTT
TTAAACAGTTTGCAAAGGCA

Downstream 100 bases:

>100_bases
GTGTTGAGTCTCACGAGGTTGTTCACACCCGGAATGCGTGTGATGGGAGGCTGATGGCCGAGCGCGGAGTGTGGCCGGCA
CCAGTTGTAGCCGTCAATGA

Product: asparaginase/glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQVVAGKILVLGTGGTIAGTAGNAKDNIGYTAAQLGIDQLLAAVPGLERAAGGARLEAEQVAQIDSKDMDGAVWRVLALR
CAEALADPEMRALVVTHGTDTLEETAWFLHRVLAGARDKPVVLVSAMRPATALAPDGPQNLLDAVSVAATPGARGVVAVA
AGEVHGAQRVQKVHPYRLNAFASGEAGPLGWMEEGRVRLAAGWPAPDCSAEADAALKALRSGAEWPRVEVVMSHAGATGR
LVDLLVADGVHGLAVAATGNGTLHHHLQAALERAQAAGVAVRVATRCSLGQLLPRPGDTLPVAQGLSVVKTRVELLLERL
AQPQA

Sequences:

>Translated_325_residues
MQVVAGKILVLGTGGTIAGTAGNAKDNIGYTAAQLGIDQLLAAVPGLERAAGGARLEAEQVAQIDSKDMDGAVWRVLALR
CAEALADPEMRALVVTHGTDTLEETAWFLHRVLAGARDKPVVLVSAMRPATALAPDGPQNLLDAVSVAATPGARGVVAVA
AGEVHGAQRVQKVHPYRLNAFASGEAGPLGWMEEGRVRLAAGWPAPDCSAEADAALKALRSGAEWPRVEVVMSHAGATGR
LVDLLVADGVHGLAVAATGNGTLHHHLQAALERAQAAGVAVRVATRCSLGQLLPRPGDTLPVAQGLSVVKTRVELLLERL
AQPQA
>Mature_325_residues
MQVVAGKILVLGTGGTIAGTAGNAKDNIGYTAAQLGIDQLLAAVPGLERAAGGARLEAEQVAQIDSKDMDGAVWRVLALR
CAEALADPEMRALVVTHGTDTLEETAWFLHRVLAGARDKPVVLVSAMRPATALAPDGPQNLLDAVSVAATPGARGVVAVA
AGEVHGAQRVQKVHPYRLNAFASGEAGPLGWMEEGRVRLAAGWPAPDCSAEADAALKALRSGAEWPRVEVVMSHAGATGR
LVDLLVADGVHGLAVAATGNGTLHHHLQAALERAQAAGVAVRVATRCSLGQLLPRPGDTLPVAQGLSVVKTRVELLLERL
AQPQA

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=264, Percent_Identity=35.2272727272727, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788065, Length=288, Percent_Identity=27.4305555555556, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320527, Length=322, Percent_Identity=29.1925465838509, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323189, Length=265, Percent_Identity=33.5849056603774, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323187, Length=265, Percent_Identity=33.5849056603774, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323186, Length=265, Percent_Identity=33.5849056603774, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323184, Length=265, Percent_Identity=33.5849056603774, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33427; Mature: 33427

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVVAGKILVLGTGGTIAGTAGNAKDNIGYTAAQLGIDQLLAAVPGLERAAGGARLEAEQ
CCEEECEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHH
VAQIDSKDMDGAVWRVLALRCAEALADPEMRALVVTHGTDTLEETAWFLHRVLAGARDKP
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCC
VVLVSAMRPATALAPDGPQNLLDAVSVAATPGARGVVAVAAGEVHGAQRVQKVHPYRLNA
EEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEE
FASGEAGPLGWMEEGRVRLAAGWPAPDCSAEADAALKALRSGAEWPRVEVVMSHAGATGR
EECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
LVDLLVADGVHGLAVAATGNGTLHHHLQAALERAQAAGVAVRVATRCSLGQLLPRPGDTL
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEHHCCHHHHCCCCCCCC
PVAQGLSVVKTRVELLLERLAQPQA
CHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQVVAGKILVLGTGGTIAGTAGNAKDNIGYTAAQLGIDQLLAAVPGLERAAGGARLEAEQ
CCEEECEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHH
VAQIDSKDMDGAVWRVLALRCAEALADPEMRALVVTHGTDTLEETAWFLHRVLAGARDKP
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCC
VVLVSAMRPATALAPDGPQNLLDAVSVAATPGARGVVAVAAGEVHGAQRVQKVHPYRLNA
EEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEE
FASGEAGPLGWMEEGRVRLAAGWPAPDCSAEADAALKALRSGAEWPRVEVVMSHAGATGR
EECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
LVDLLVADGVHGLAVAATGNGTLHHHLQAALERAQAAGVAVRVATRCSLGQLLPRPGDTL
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEHHCCHHHHCCCCCCCC
PVAQGLSVVKTRVELLLERLAQPQA
CHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]