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The map label for this gene is dusA [H]

Identifier: 120611162

GI number: 120611162

Start: 2727883

End: 2728926

Strand: Reverse

Name: dusA [H]

Synonym: Aave_2491

Alternate gene names: 120611162

Gene position: 2728926-2727883 (Counterclockwise)

Preceding gene: 120611163

Following gene: 120611160

Centisome position: 50.98

GC content: 68.97

Gene sequence:

>1044_bases
ATGTTACAAAATCCCTCGCAAGCTATTGATTCTAAACAAACCAGCCATGCAGCTGGCCGTTGGCGGATTTCTGTAGCCCC
CATGATGGACTGGACCGACCGCCACTGCCGCTACTTCCACCGGTTGCTGAGCCGCCATGCCCTGCTCTACACCGAGATGG
TGACAACCGGCGCGCTGGTGCATGGCGACGTGGAGCGGCACCTGCGCTTCAACGCCGAGGAGCACCCGGTCGCCCTGCAG
CTGGGCGGCAGCGAGCCGGGCGACCTCGCGCATTCAGCGCGTCTGGGCGAGCGCTGGGGCTATGGCGAGATCAATCTCAA
CTGCGGATGCCCCAGCGAGCGCGTGCAGCGCGGGGCCTTCGGCGCCTGCCTGATGAACGAGCCGCGCCTGGTGGCCGACT
GCGTGAAGGCGATGGTGGATGCGGTGAGCGTGCCCGTCACCGTCAAGCACCGCATCGGCATCGGCCGCAGCGAGGAGTAC
GCCTTCGTGCGGGATTTCGTGGGCACCGTGGCCGAGGCCGGCTGCCGCGTGTTCATCGTGCATGCGCGCAATGCCTGGCT
CGAAGGCCTGTCTCCCAAGGAGAACCGTGAAATCCCGCCGCTGCGCTACGACGTGGTGCACCGGCTCAAGGCCGAGTTCC
CGGATCTCGTGATCGCGATCAACGGTGGACTCGCGACCGATGCCGCAGTGCAGGAAGCCCTGGGCGGCGGGCTGGACGGC
GCGATGATCGGCCGCGAGGCCTACCACAACCCCTGGTGGCTCGCCCGCTGGGATGCGCTGTACTTCGGCGATGCCGCGTC
CGGACTCACCCGGGAGGCCGCCGAAGAGGCCATGGTGGACTACATGGCGCGCGAGGCGGCGCAGCACGGCACCTCCTGGT
ACTCCGTCGCGCGCCACATGCTGGGCCTGCGCAACGGGTTGCCCGGCGCGCGGCGGTGGCGGCAGGTCTGGAGCGACCAC
CGCCTCAAGCACCTGCCCGCGCACGAGGTGATGGCCATCGCGCGCACGCCGCCCCGGGTGGCGGCGGACGCCCTGGCCGC
CTGA

Upstream 100 bases:

>100_bases
TTGTAGTGCTACATGGCCGTCTGTGTAGCACTCAATGTAGCACCGCGGCCGTAAAAATGGGGCGGAATGGTGCGGGAAAG
CGGAAAATAGGAAGCCAACA

Downstream 100 bases:

>100_bases
ATACCGCAGGCCGCGGTTCAGACCGCCGCTGCACCCACCTGCGTGTCGATCCACTCCTTGAGCGGCAGGGGGTGGGCGTA
CAGATAGCCCTGCATCGCGT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLQNPSQAIDSKQTSHAAGRWRISVAPMMDWTDRHCRYFHRLLSRHALLYTEMVTTGALVHGDVERHLRFNAEEHPVALQ
LGGSEPGDLAHSARLGERWGYGEINLNCGCPSERVQRGAFGACLMNEPRLVADCVKAMVDAVSVPVTVKHRIGIGRSEEY
AFVRDFVGTVAEAGCRVFIVHARNAWLEGLSPKENREIPPLRYDVVHRLKAEFPDLVIAINGGLATDAAVQEALGGGLDG
AMIGREAYHNPWWLARWDALYFGDAASGLTREAAEEAMVDYMAREAAQHGTSWYSVARHMLGLRNGLPGARRWRQVWSDH
RLKHLPAHEVMAIARTPPRVAADALAA

Sequences:

>Translated_347_residues
MLQNPSQAIDSKQTSHAAGRWRISVAPMMDWTDRHCRYFHRLLSRHALLYTEMVTTGALVHGDVERHLRFNAEEHPVALQ
LGGSEPGDLAHSARLGERWGYGEINLNCGCPSERVQRGAFGACLMNEPRLVADCVKAMVDAVSVPVTVKHRIGIGRSEEY
AFVRDFVGTVAEAGCRVFIVHARNAWLEGLSPKENREIPPLRYDVVHRLKAEFPDLVIAINGGLATDAAVQEALGGGLDG
AMIGREAYHNPWWLARWDALYFGDAASGLTREAAEEAMVDYMAREAAQHGTSWYSVARHMLGLRNGLPGARRWRQVWSDH
RLKHLPAHEVMAIARTPPRVAADALAA
>Mature_347_residues
MLQNPSQAIDSKQTSHAAGRWRISVAPMMDWTDRHCRYFHRLLSRHALLYTEMVTTGALVHGDVERHLRFNAEEHPVALQ
LGGSEPGDLAHSARLGERWGYGEINLNCGCPSERVQRGAFGACLMNEPRLVADCVKAMVDAVSVPVTVKHRIGIGRSEEY
AFVRDFVGTVAEAGCRVFIVHARNAWLEGLSPKENREIPPLRYDVVHRLKAEFPDLVIAINGGLATDAAVQEALGGGLDG
AMIGREAYHNPWWLARWDALYFGDAASGLTREAAEEAMVDYMAREAAQHGTSWYSVARHMLGLRNGLPGARRWRQVWSDH
RLKHLPAHEVMAIARTPPRVAADALAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=250, Percent_Identity=28.4, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=57.0967741935484, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1789660, Length=238, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=29.045643153527, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38460; Mature: 38460

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNPSQAIDSKQTSHAAGRWRISVAPMMDWTDRHCRYFHRLLSRHALLYTEMVTTGALV
CCCCCHHHHHCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
HGDVERHLRFNAEEHPVALQLGGSEPGDLAHSARLGERWGYGEINLNCGCPSERVQRGAF
CCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCC
GACLMNEPRLVADCVKAMVDAVSVPVTVKHRIGIGRSEEYAFVRDFVGTVAEAGCRVFIV
CHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
HARNAWLEGLSPKENREIPPLRYDVVHRLKAEFPDLVIAINGGLATDAAVQEALGGGLDG
ECCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCH
AMIGREAYHNPWWLARWDALYFGDAASGLTREAAEEAMVDYMAREAAQHGTSWYSVARHM
HHHCHHHCCCCCEEEEECEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGLRNGLPGARRWRQVWSDHRLKHLPAHEVMAIARTPPRVAADALAA
HHHHCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLQNPSQAIDSKQTSHAAGRWRISVAPMMDWTDRHCRYFHRLLSRHALLYTEMVTTGALV
CCCCCHHHHHCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
HGDVERHLRFNAEEHPVALQLGGSEPGDLAHSARLGERWGYGEINLNCGCPSERVQRGAF
CCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCC
GACLMNEPRLVADCVKAMVDAVSVPVTVKHRIGIGRSEEYAFVRDFVGTVAEAGCRVFIV
CHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
HARNAWLEGLSPKENREIPPLRYDVVHRLKAEFPDLVIAINGGLATDAAVQEALGGGLDG
ECCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCH
AMIGREAYHNPWWLARWDALYFGDAASGLTREAAEEAMVDYMAREAAQHGTSWYSVARHM
HHHCHHHCCCCCEEEEECEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGLRNGLPGARRWRQVWSDHRLKHLPAHEVMAIARTPPRVAADALAA
HHHHCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]