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The map label for this gene is maiA [H]

Identifier: 120611145

GI number: 120611145

Start: 2705958

End: 2706626

Strand: Direct

Name: maiA [H]

Synonym: Aave_2474

Alternate gene names: 120611145

Gene position: 2705958-2706626 (Clockwise)

Preceding gene: 120611141

Following gene: 120611146

Centisome position: 50.55

GC content: 71.9

Gene sequence:

>669_bases
ATGAAGCTGTTCAACTATTTCCGCTCTTCCGCGTCCTACCGGGTGCGCATCGCCCTGGCGCTGAAGGGGCTGCCCTACGA
GTACGTGCCGGTGCACCTGGTGCGCGCGGAGCACCGCGCGCCCGGCTACGCGGACCGCGTCGGGGATGCGCTCGTGCCGG
CGCTGGAGCTGGACGACGGGAGCACGCTCGTGCAGTCGATGGCGATCATCGAATACCTGGACGAAACGCATCCGCAGCCG
CCGCTGCTGCCCGGCGACGCGCTCGGCCGGGCCCACGTGCGGGCACTGGCCCAGATGGTGGCCTGCGAGATCCACCCGGT
CAACAACCTGCGGGTGCTCAAGTACCTCACCCAGACGCTGGGGCACGACGAAGCGGAGAAGACCGCCTGGTACCGCCACT
GGGCGCGCGGTGGCCTGGAGGCGTTCGAGCGGCAGCTCGGCCTGCTGGCCCGTGCCCGGGAGGCAGCGGGGCTGCCGCCG
TCGCGGCTGTGCTGGGGCGATGCCCCGACGCTCGCCGACTGCTGCCTGGTGCCGCAAATCTTCAACGCGCAGCGCTTCGA
GGTCGATCTGGCCGGGCTGCCGCGCACCATGGCGGCCTGGGAGGCCGCGTCCGCGCTGCCGGCATTCCAGGCGGCCCATC
CCTCCGCATGCCCCGATGCCGGGGGCTGA

Upstream 100 bases:

>100_bases
GGCCTTGTCTCGGTGGGGTGCGGTGGCCCGTGCGCGCCGTGCAGGCGCCGGCAGGCCCCGGCCAGAGTGTGCCATGCACG
GACGTTATGCTTGGCGGACC

Downstream 100 bases:

>100_bases
CGCCTTGCCGCCCGAGGTTGCTTCCCTGCCGGAAGGCTGGCTGCGGCCCGACTGGCCCGCGCCCGCCCACGTGCACGCGG
TGTGCACGGCACGGCCGGGC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKLFNYFRSSASYRVRIALALKGLPYEYVPVHLVRAEHRAPGYADRVGDALVPALELDDGSTLVQSMAIIEYLDETHPQP
PLLPGDALGRAHVRALAQMVACEIHPVNNLRVLKYLTQTLGHDEAEKTAWYRHWARGGLEAFERQLGLLARAREAAGLPP
SRLCWGDAPTLADCCLVPQIFNAQRFEVDLAGLPRTMAAWEAASALPAFQAAHPSACPDAGG

Sequences:

>Translated_222_residues
MKLFNYFRSSASYRVRIALALKGLPYEYVPVHLVRAEHRAPGYADRVGDALVPALELDDGSTLVQSMAIIEYLDETHPQP
PLLPGDALGRAHVRALAQMVACEIHPVNNLRVLKYLTQTLGHDEAEKTAWYRHWARGGLEAFERQLGLLARAREAAGLPP
SRLCWGDAPTLADCCLVPQIFNAQRFEVDLAGLPRTMAAWEAASALPAFQAAHPSACPDAGG
>Mature_222_residues
MKLFNYFRSSASYRVRIALALKGLPYEYVPVHLVRAEHRAPGYADRVGDALVPALELDDGSTLVQSMAIIEYLDETHPQP
PLLPGDALGRAHVRALAQMVACEIHPVNNLRVLKYLTQTLGHDEAEKTAWYRHWARGGLEAFERQLGLLARAREAAGLPP
SRLCWGDAPTLADCCLVPQIFNAQRFEVDLAGLPRTMAAWEAASALPAFQAAHPSACPDAGG

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=220, Percent_Identity=40.9090909090909, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI22202622, Length=169, Percent_Identity=40.2366863905325, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17551302, Length=220, Percent_Identity=40.4545454545455, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17510461, Length=219, Percent_Identity=34.2465753424658, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17556142, Length=208, Percent_Identity=34.6153846153846, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21355859, Length=220, Percent_Identity=41.3636363636364, Blast_Score=163, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24645375, Length=220, Percent_Identity=41.3636363636364, Blast_Score=163, Evalue=8e-41,
Organism=Drosophila melanogaster, GI45553325, Length=220, Percent_Identity=41.3636363636364, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21355857, Length=221, Percent_Identity=36.6515837104072, Blast_Score=140, Evalue=7e-34,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24289; Mature: 24289

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFNYFRSSASYRVRIALALKGLPYEYVPVHLVRAEHRAPGYADRVGDALVPALELDDG
CCHHHHHHCCCCEEEEEEEEECCCCCCHHHHEEEECCCCCCCHHHHHHHHHCCEEECCCC
STLVQSMAIIEYLDETHPQPPLLPGDALGRAHVRALAQMVACEIHPVNNLRVLKYLTQTL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GHDEAEKTAWYRHWARGGLEAFERQLGLLARAREAAGLPPSRLCWGDAPTLADCCLVPQI
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FNAQRFEVDLAGLPRTMAAWEAASALPAFQAAHPSACPDAGG
HCCCCEEEECCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLFNYFRSSASYRVRIALALKGLPYEYVPVHLVRAEHRAPGYADRVGDALVPALELDDG
CCHHHHHHCCCCEEEEEEEEECCCCCCHHHHEEEECCCCCCCHHHHHHHHHCCEEECCCC
STLVQSMAIIEYLDETHPQPPLLPGDALGRAHVRALAQMVACEIHPVNNLRVLKYLTQTL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GHDEAEKTAWYRHWARGGLEAFERQLGLLARAREAAGLPPSRLCWGDAPTLADCCLVPQI
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FNAQRFEVDLAGLPRTMAAWEAASALPAFQAAHPSACPDAGG
HCCCCEEEECCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]