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The map label for this gene is gcvA [H]

Identifier: 120611095

GI number: 120611095

Start: 2646146

End: 2647114

Strand: Reverse

Name: gcvA [H]

Synonym: Aave_2424

Alternate gene names: 120611095

Gene position: 2647114-2646146 (Counterclockwise)

Preceding gene: 120611103

Following gene: 120611093

Centisome position: 49.45

GC content: 69.56

Gene sequence:

>969_bases
ATGGATTCACGCCTGTCCAATTCGGTTCTGGCCTGGCTGCGCTGTTTCGATGCGGCCGCACGGCAGGGCAGCTTCACACG
CGCCGCCGCGGAGCTGTGCATCACCCAGGGCGCGGTGAGCCAGAAGGTGAAGCAACTGGAACGCTGGCTGGGACGTCCCC
TGTTCCTGCGCACCCCGCGGTTACTGGTGCCCACCCCGGAGGGCAAATGGCTGGCCGTGGTGCTGCGCGAAAGCTTCGAG
GCCATCGAGGGCACGCTCGCGCAGATGCGGCGCTCCACCCCGGCCCACGCTGCAGCCACGCTGAGCTGCTCACCTTCGTT
CGCCATGCAGTGGCTTACGCCACGGCTGGGCGAATTCTTCCGCCGGCACCCCGACACCGGGCTGCGCGTGTTCGGAGAGT
TCCACCGCATCGACCGCACCCGCATGGTGCGCGACGGGGTCGAGGCGGCCGTCCGCTTCGACCCGGAGGAGTACACCGAC
CTGGACGCCACCGGGTTCCTCGATGAATGGCTGGTCCCCGTGGCCAGCCCGGCTTTCGTAGCGGCCCACCCGGACCTGCG
GGATGCGCCGGCCAGGCTGCGGCCGGAATGGCTGCTGCACGACGGCAGCGCCTGGGAAGGAGCCGACACCTTCGAGGAGT
GGCAGCACTGGTTCGCCGCGCAGGGTGCGCCATGCCCGGACTGGGGAGGCGGCCCCCAGTTCAACCTGTCCCAGCTGGCG
GTGGGAGCAGCCATCACCGGCCAGGGCATCGCCATGGGTCGTGCCGCGCTCGTGCTCGAAGACGTGGCGGCCGGCCGGCT
CGTACCGCTGTGTTCCTGGAGCACGCTCTCCCGGGCGCGCTACGCCTTCGTGAGTTCGCCCCAGGCGGGGCCTGCCATGC
TGCGCGTGCGGGACTGGCTGGTGGAGGAAGGGCAGCGCTTCAAAGAGGCGCGTGCGCAGGTGCTGCCACCATTGTTAATC
TGCATCTAG

Upstream 100 bases:

>100_bases
AGTTCGGTCATGGAACGGTAAAGAAAGCGTTGCACGCATTCTTGTGCCGGCCCATGGGCCGTGCAATGCATTTATATTGA
CGACAGCATTAGTTTCATTG

Downstream 100 bases:

>100_bases
AGCGTGTTATGAACTTCCCGCCGCAGCCAGCACCCGTGCGGCTGCAGGCAACATGAGCACGGCAAAGACCAGGAAATGCA
GCCCGCCGAGCACTTCGGGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MDSRLSNSVLAWLRCFDAAARQGSFTRAAAELCITQGAVSQKVKQLERWLGRPLFLRTPRLLVPTPEGKWLAVVLRESFE
AIEGTLAQMRRSTPAHAAATLSCSPSFAMQWLTPRLGEFFRRHPDTGLRVFGEFHRIDRTRMVRDGVEAAVRFDPEEYTD
LDATGFLDEWLVPVASPAFVAAHPDLRDAPARLRPEWLLHDGSAWEGADTFEEWQHWFAAQGAPCPDWGGGPQFNLSQLA
VGAAITGQGIAMGRAALVLEDVAAGRLVPLCSWSTLSRARYAFVSSPQAGPAMLRVRDWLVEEGQRFKEARAQVLPPLLI
CI

Sequences:

>Translated_322_residues
MDSRLSNSVLAWLRCFDAAARQGSFTRAAAELCITQGAVSQKVKQLERWLGRPLFLRTPRLLVPTPEGKWLAVVLRESFE
AIEGTLAQMRRSTPAHAAATLSCSPSFAMQWLTPRLGEFFRRHPDTGLRVFGEFHRIDRTRMVRDGVEAAVRFDPEEYTD
LDATGFLDEWLVPVASPAFVAAHPDLRDAPARLRPEWLLHDGSAWEGADTFEEWQHWFAAQGAPCPDWGGGPQFNLSQLA
VGAAITGQGIAMGRAALVLEDVAAGRLVPLCSWSTLSRARYAFVSSPQAGPAMLRVRDWLVEEGQRFKEARAQVLPPLLI
CI
>Mature_322_residues
MDSRLSNSVLAWLRCFDAAARQGSFTRAAAELCITQGAVSQKVKQLERWLGRPLFLRTPRLLVPTPEGKWLAVVLRESFE
AIEGTLAQMRRSTPAHAAATLSCSPSFAMQWLTPRLGEFFRRHPDTGLRVFGEFHRIDRTRMVRDGVEAAVRFDPEEYTD
LDATGFLDEWLVPVASPAFVAAHPDLRDAPARLRPEWLLHDGSAWEGADTFEEWQHWFAAQGAPCPDWGGGPQFNLSQLA
VGAAITGQGIAMGRAALVLEDVAAGRLVPLCSWSTLSRARYAFVSSPQAGPAMLRVRDWLVEEGQRFKEARAQVLPPLLI
CI

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=276, Percent_Identity=35.8695652173913, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1788706, Length=279, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786448, Length=305, Percent_Identity=29.1803278688525, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=27.6595744680851, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787589, Length=165, Percent_Identity=31.5151515151515, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789283, Length=301, Percent_Identity=27.906976744186, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35718; Mature: 35718

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSRLSNSVLAWLRCFDAAARQGSFTRAAAELCITQGAVSQKVKQLERWLGRPLFLRTPR
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCE
LLVPTPEGKWLAVVLRESFEAIEGTLAQMRRSTPAHAAATLSCSPSFAMQWLTPRLGEFF
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCHHHHHHHHHHHHHHHH
RRHPDTGLRVFGEFHRIDRTRMVRDGVEAAVRFDPEEYTDLDATGFLDEWLVPVASPAFV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCCCCHHHHHHHCCCCCEE
AAHPDLRDAPARLRPEWLLHDGSAWEGADTFEEWQHWFAAQGAPCPDWGGGPQFNLSQLA
EECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
VGAAITGQGIAMGRAALVLEDVAAGRLVPLCSWSTLSRARYAFVSSPQAGPAMLRVRDWL
HHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
VEEGQRFKEARAQVLPPLLICI
HHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MDSRLSNSVLAWLRCFDAAARQGSFTRAAAELCITQGAVSQKVKQLERWLGRPLFLRTPR
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCE
LLVPTPEGKWLAVVLRESFEAIEGTLAQMRRSTPAHAAATLSCSPSFAMQWLTPRLGEFF
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCHHHHHHHHHHHHHHHH
RRHPDTGLRVFGEFHRIDRTRMVRDGVEAAVRFDPEEYTDLDATGFLDEWLVPVASPAFV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCCCCHHHHHHHCCCCCEE
AAHPDLRDAPARLRPEWLLHDGSAWEGADTFEEWQHWFAAQGAPCPDWGGGPQFNLSQLA
EECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
VGAAITGQGIAMGRAALVLEDVAAGRLVPLCSWSTLSRARYAFVSSPQAGPAMLRVRDWL
HHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
VEEGQRFKEARAQVLPPLLICI
HHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]