Click here to switch to the map view.

The map label for this gene is rluE [H]

Identifier: 120610961

GI number: 120610961

Start: 2518640

End: 2519299

Strand: Reverse

Name: rluE [H]

Synonym: Aave_2287

Alternate gene names: 120610961

Gene position: 2519299-2518640 (Counterclockwise)

Preceding gene: 120610962

Following gene: 120610960

Centisome position: 47.07

GC content: 74.7

Gene sequence:

>660_bases
ATGCGGCCTGGCGACGGCCGGCGCAGACCCAGCCCGCGGCCCGGGGTGCGGCTCGAACCGGTGGAACGGGCCGGGCCGTC
CCGCCTGCTGCGCTTCCACAAGCCCTACGGCGTGCTGAGCCAGTTCACGCCCGAGGGCCGCTGGCGCGGGCTGAAGGATT
TCATCGACCTGCCGGGCGTGTACGTGGCGGGCCGGCTGGATGCCGACAGCGAGGGGCTGCTGCTGCTGACCGACGACGGC
GGGCTGCAGGCGCGCATCGCCGATCCGCGCTTCAAGATGGAAAAGACCTACTGGGTGCAGGTGGAGGGCGTGCCGGATGC
GGCGGCCCTGCAGGCGCTCGAGCGCGGGGTGCAGCTGAACGACGGCCCGACGCTGCCGGCGCGCGCCCGGCGCCTGGATG
TGCCGCCGCCCGTGGGCGAGCGCGAGCCGCCGATCCGCGTGCGGCAGCACATTCCGGACACCTGGATCGAGCTGGTGATC
CGCGAAGGCCGCAACCGGCAGGTGCGGCGCATGACGGCGGCGGTGGGGCATCCCACGCTGCGGCTGGTGCGCGCGGCGAT
CGGCCCCTTCGCGCTCGACGGGCTGGCACCCGGCACCTGGGCAGAGGCGGAAATTCCCGCGGCCCTCGCGGTCCGGCGGC
CCGGTACCCACCGGATGTGA

Upstream 100 bases:

>100_bases
GGCCGCACCTGCACTGGGGCGTGATGCTGAACCGGACCATGGTGGATCCGGCGTTGTTCCTGCCGGCATGACGCGCCGCG
AACATGGCGCTCCGCCGCCG

Downstream 100 bases:

>100_bases
CGCCGGGCGGCAGCGGGTCGCGGCGAACCGGGCAATGACCGCGCAGGCGGCTGCGCAGGAGAGACAATCGCGGCCATTCC
ACCGCGCACGGCCGCTGCAG

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MRPGDGRRRPSPRPGVRLEPVERAGPSRLLRFHKPYGVLSQFTPEGRWRGLKDFIDLPGVYVAGRLDADSEGLLLLTDDG
GLQARIADPRFKMEKTYWVQVEGVPDAAALQALERGVQLNDGPTLPARARRLDVPPPVGEREPPIRVRQHIPDTWIELVI
REGRNRQVRRMTAAVGHPTLRLVRAAIGPFALDGLAPGTWAEAEIPAALAVRRPGTHRM

Sequences:

>Translated_219_residues
MRPGDGRRRPSPRPGVRLEPVERAGPSRLLRFHKPYGVLSQFTPEGRWRGLKDFIDLPGVYVAGRLDADSEGLLLLTDDG
GLQARIADPRFKMEKTYWVQVEGVPDAAALQALERGVQLNDGPTLPARARRLDVPPPVGEREPPIRVRQHIPDTWIELVI
REGRNRQVRRMTAAVGHPTLRLVRAAIGPFALDGLAPGTWAEAEIPAALAVRRPGTHRM
>Mature_219_residues
MRPGDGRRRPSPRPGVRLEPVERAGPSRLLRFHKPYGVLSQFTPEGRWRGLKDFIDLPGVYVAGRLDADSEGLLLLTDDG
GLQARIADPRFKMEKTYWVQVEGVPDAAALQALERGVQLNDGPTLPARARRLDVPPPVGEREPPIRVRQHIPDTWIELVI
REGRNRQVRRMTAAVGHPTLRLVRAAIGPFALDGLAPGTWAEAEIPAALAVRRPGTHRM

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=196, Percent_Identity=54.0816326530612, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1788510, Length=196, Percent_Identity=37.7551020408163, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787524, Length=190, Percent_Identity=35.7894736842105, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790453, Length=173, Percent_Identity=28.3236994219653, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24317; Mature: 24317

Theoretical pI: Translated: 11.35; Mature: 11.35

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGDGRRRPSPRPGVRLEPVERAGPSRLLRFHKPYGVLSQFTPEGRWRGLKDFIDLPGV
CCCCCCCCCCCCCCCCEECCHHHCCHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHCCCCE
YVAGRLDADSEGLLLLTDDGGLQARIADPRFKMEKTYWVQVEGVPDAAALQALERGVQLN
EEEEECCCCCCCEEEEECCCCCEEEECCCCEEECEEEEEEEECCCCHHHHHHHHCCCCCC
DGPTLPARARRLDVPPPVGEREPPIRVRQHIPDTWIELVIREGRNRQVRRMTAAVGHPTL
CCCCCCHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
RLVRAAIGPFALDGLAPGTWAEAEIPAALAVRRPGTHRM
HHHHHHHCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MRPGDGRRRPSPRPGVRLEPVERAGPSRLLRFHKPYGVLSQFTPEGRWRGLKDFIDLPGV
CCCCCCCCCCCCCCCCEECCHHHCCHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHCCCCE
YVAGRLDADSEGLLLLTDDGGLQARIADPRFKMEKTYWVQVEGVPDAAALQALERGVQLN
EEEEECCCCCCCEEEEECCCCCEEEECCCCEEECEEEEEEEECCCCHHHHHHHHCCCCCC
DGPTLPARARRLDVPPPVGEREPPIRVRQHIPDTWIELVIREGRNRQVRRMTAAVGHPTL
CCCCCCHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
RLVRAAIGPFALDGLAPGTWAEAEIPAALAVRRPGTHRM
HHHHHHHCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]