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The map label for this gene is dgoD [H]

Identifier: 120610918

GI number: 120610918

Start: 2467448

End: 2468596

Strand: Reverse

Name: dgoD [H]

Synonym: Aave_2244

Alternate gene names: 120610918

Gene position: 2468596-2467448 (Counterclockwise)

Preceding gene: 120610919

Following gene: 120610916

Centisome position: 46.12

GC content: 68.93

Gene sequence:

>1149_bases
ATGAAGATCACCCGATTGACCACGTTCCTGGTGCCGCCGCGCTGGTGCTTCCTGAAGATCGAAACCGACGAAGGCATCGA
CGGCTGGGGCGAGCCCATCCTCGAAGGCCGTGCCCACACCGTGGCCGCCGCCGTGGAGGAACTGGCCGACTACCTCGTCG
GCAAGGACCCGCGCAACATCGAGGACCACTGGACGGTGCTCTACCGCGGCGGCTTCTACCGCGGCGGTGGCATCCACATG
AGCGCGCTGGCCGGCATCGACCAGGCGCTGTGGGACATCAAGGGCAAGGCCCTGGGCGTGCCGGTGTCCGAGCTGCTGGG
CGGCGGCGTGCGCGACCGCATCCGCGTCTACAGCTGGATCGGTGGCGACCGGCCGGCCGAGACCGCGCGCCAGGCCCGCG
ACGCGGTGGGCCGGGGCTTCACCGCGGTGAAGATGAACGGAACGGAAGAAATGCAGTTCGTGGACACGTTCGACAAGGTG
GAGCGCTGCCTGCAGAACGTGGCCGCCGTGCGCGAGGCCGTGGGCCCGCACGTGGGCATCGGCGTGGACTTCCACGGCCG
CGTCCACCGGCCCATGGCCAAGGTGCTCATCAAGGAACTGGAGCCCTACCGGCTCATGTTCATCGAGGAGCCCGTGCTCA
GCGAGCACGACGAGGCCCTCAAGGAGATCGCGCGCATCTCGTCCACGCCCATCGCGCTGGGCGAGCGGCTCTACTCGCGC
TGGGACTTCAAGCGCGTGCTGCAGGAGGGCTATGCGGACATCGTCCAGCCCGACCCGTCCCATGCCGGCGGCATCACCGA
AACGCGCAAGATCGCGTCCATGGCCGAGGCCTACGACGTGGCCCTGGCGCTGCACTGCCCCCTGGGGCCCATCGCGCTGG
CGGCCAACCTGCAGCTCGACGCGGTCTGCTACAACGCCTTCATCCAGGAGCAGAGCCTCGGCATCCACTACAACCAGGAC
AACGACCTGATGGACTACGTGTCCAACCCGCAGGTCTTCGCCTACCAGGACGGCATGGTGGCCATCCCGCGCGGCCCGGG
CCTGGGCATCGAGGTCAACGAAGACTACGTGCGCGAGCGCGCCGCGCAGGGCCACCGCTGGCGCAACCCGGTGTGGCGCC
ACCGGGACGGCAGCCTCGCGGAATGGTGA

Upstream 100 bases:

>100_bases
GGCATGGCCCGCACCTGCGGACAGGGCGCCGCGCCGGCCGCCGCGGCCACCCGTTCCTCTCCCGACGCAGCCACCGTTTC
CTGAGATTCCCCCCCCTCCC

Downstream 100 bases:

>100_bases
TGCCCGCCACGGGCGGCCTGCGCCCCCGCCGGCGCTAGGATCCGCCGGTCGCCACGGCCTCGCGCGCCGCGGCGCGCACC
TGCCCGGCCCGTTCGGTGGC

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITRLTTFLVPPRWCFLKIETDEGIDGWGEPILEGRAHTVAAAVEELADYLVGKDPRNIEDHWTVLYRGGFYRGGGIHM
SALAGIDQALWDIKGKALGVPVSELLGGGVRDRIRVYSWIGGDRPAETARQARDAVGRGFTAVKMNGTEEMQFVDTFDKV
ERCLQNVAAVREAVGPHVGIGVDFHGRVHRPMAKVLIKELEPYRLMFIEEPVLSEHDEALKEIARISSTPIALGERLYSR
WDFKRVLQEGYADIVQPDPSHAGGITETRKIASMAEAYDVALALHCPLGPIALAANLQLDAVCYNAFIQEQSLGIHYNQD
NDLMDYVSNPQVFAYQDGMVAIPRGPGLGIEVNEDYVRERAAQGHRWRNPVWRHRDGSLAEW

Sequences:

>Translated_382_residues
MKITRLTTFLVPPRWCFLKIETDEGIDGWGEPILEGRAHTVAAAVEELADYLVGKDPRNIEDHWTVLYRGGFYRGGGIHM
SALAGIDQALWDIKGKALGVPVSELLGGGVRDRIRVYSWIGGDRPAETARQARDAVGRGFTAVKMNGTEEMQFVDTFDKV
ERCLQNVAAVREAVGPHVGIGVDFHGRVHRPMAKVLIKELEPYRLMFIEEPVLSEHDEALKEIARISSTPIALGERLYSR
WDFKRVLQEGYADIVQPDPSHAGGITETRKIASMAEAYDVALALHCPLGPIALAANLQLDAVCYNAFIQEQSLGIHYNQD
NDLMDYVSNPQVFAYQDGMVAIPRGPGLGIEVNEDYVRERAAQGHRWRNPVWRHRDGSLAEW
>Mature_382_residues
MKITRLTTFLVPPRWCFLKIETDEGIDGWGEPILEGRAHTVAAAVEELADYLVGKDPRNIEDHWTVLYRGGFYRGGGIHM
SALAGIDQALWDIKGKALGVPVSELLGGGVRDRIRVYSWIGGDRPAETARQARDAVGRGFTAVKMNGTEEMQFVDTFDKV
ERCLQNVAAVREAVGPHVGIGVDFHGRVHRPMAKVLIKELEPYRLMFIEEPVLSEHDEALKEIARISSTPIALGERLYSR
WDFKRVLQEGYADIVQPDPSHAGGITETRKIASMAEAYDVALALHCPLGPIALAANLQLDAVCYNAFIQEQSLGIHYNQD
NDLMDYVSNPQVFAYQDGMVAIPRGPGLGIEVNEDYVRERAAQGHRWRNPVWRHRDGSLAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=58.9005235602094, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1787864, Length=413, Percent_Identity=30.9927360774818, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI226510960, Length=238, Percent_Identity=31.0924369747899, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42639; Mature: 42639

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRLTTFLVPPRWCFLKIETDEGIDGWGEPILEGRAHTVAAAVEELADYLVGKDPRNI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
EDHWTVLYRGGFYRGGGIHMSALAGIDQALWDIKGKALGVPVSELLGGGVRDRIRVYSWI
CCCEEEEECCCEECCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHEEEEEC
GGDRPAETARQARDAVGRGFTAVKMNGTEEMQFVDTFDKVERCLQNVAAVREAVGPHVGI
CCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GVDFHGRVHRPMAKVLIKELEPYRLMFIEEPVLSEHDEALKEIARISSTPIALGERLYSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WDFKRVLQEGYADIVQPDPSHAGGITETRKIASMAEAYDVALALHCPLGPIALAANLQLD
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCHH
AVCYNAFIQEQSLGIHYNQDNDLMDYVSNPQVFAYQDGMVAIPRGPGLGIEVNEDYVRER
HHHHHHHHHHCCCCEEECCCCCHHHHCCCCCEEEEECCEEEECCCCCCCEEECHHHHHHH
AAQGHRWRNPVWRHRDGSLAEW
HHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITRLTTFLVPPRWCFLKIETDEGIDGWGEPILEGRAHTVAAAVEELADYLVGKDPRNI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
EDHWTVLYRGGFYRGGGIHMSALAGIDQALWDIKGKALGVPVSELLGGGVRDRIRVYSWI
CCCEEEEECCCEECCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHEEEEEC
GGDRPAETARQARDAVGRGFTAVKMNGTEEMQFVDTFDKVERCLQNVAAVREAVGPHVGI
CCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GVDFHGRVHRPMAKVLIKELEPYRLMFIEEPVLSEHDEALKEIARISSTPIALGERLYSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WDFKRVLQEGYADIVQPDPSHAGGITETRKIASMAEAYDVALALHCPLGPIALAANLQLD
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCHH
AVCYNAFIQEQSLGIHYNQDNDLMDYVSNPQVFAYQDGMVAIPRGPGLGIEVNEDYVRER
HHHHHHHHHHCCCCEEECCCCCHHHHCCCCCEEEEECCEEEECCCCCCCEEECHHHHHHH
AAQGHRWRNPVWRHRDGSLAEW
HHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA