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The map label for this gene is flhA [H]

Identifier: 120610869

GI number: 120610869

Start: 2416080

End: 2417186

Strand: Reverse

Name: flhA [H]

Synonym: Aave_2195

Alternate gene names: 120610869

Gene position: 2417186-2416080 (Counterclockwise)

Preceding gene: 120610873

Following gene: 120610868

Centisome position: 45.16

GC content: 65.76

Gene sequence:

>1107_bases
ATGAAGACCAAAGCAGCCGTCGCCTGGAAATCCGGCGAACCCCTGACCATCGAAACCGTGGACCTCGAAGGCCCGAAGTT
CGGCGAGGTGCTGGTGGAGATCAAGGCCACGGGCATCTGCCACACCGATTACTACACCCTCTCGGGCGCCGATCCGGAAG
GCATCTTCCCGGCCATACTGGGCCACGAAGGCGCGGGCGTGGTGGTGGACGTGGGCCCGGGCGTCACCTCGCTCAAGAAG
GGCGACCACGTGATCCCGCTCTACACGCCCGAGTGCCGGCAGTGCAAGTTCTGCCTCTCGCGCAAGACCAACCTGTGCCA
GCTGATCCGCGGCACGCAGGGCAAGGGCCTGATGCCCGACGCCACGAGCCGCTTCAGCCTGGACGGCCAGCCGATCTTCC
ACTACATGGGCACCAGCACGTTCAGCAACTACACGGTGGCGCCGGAGATCTCGCTGGCCAGGATCCGCGAGGACGCGCCC
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTGACCACCGGCATCGGCGCCGTGCTGTTCACGGCCAAGGTGGAGGCCGG
CGCGAACGTGGTGGTGTTCGGCCTGGGCGGCATCGGCCTGAACGTGATCCAGGGCGCGAAGATGGTGGGCGCGGACAAGA
TCATCGGCGTGGACATCAACCCCGCCCGCCAGGAGATGGCCCGCCAGTTCGGGATGACGCACTTCATCAACCCGAACGAG
GTGGAGAACGTGGTGGACGCCATCGTGCAGCTGACCGACGGCGGCGCGGACTATTCGTTCGAGTGCATCGGCAACACCAA
GGTCATGCGCCAGGCGCTGGAGTGCACGCACAAGGGCTGGGGGCAGAGCATCATCATCGGCGTGGCCGAGGCCGGCGCGG
AGATCAGCACCCGCCCCTTCCAGCTCGTCACGGGCCGGCAGTGGAAGGGTTCGGCCTTCGGCGGCGCGCGCGGCCGCACC
GACGTGCCGAAGATCGTGGACTGGTACATGGAAGGCAAGATCGACATCGACAGCCTCATCACCCACACGATGCCCCTGGA
GGACATCAACAAGGGCTTCGATCTGATGAAACGCGGCGAGTCGATCCGCGGCGTCGTCGTTTACTGA

Upstream 100 bases:

>100_bases
GCCCGGCCGGCCGGGGCATGCGCGGATTGTCCCCGATCGACGCGCCGCCCACGGGGCCGGCCGGCCGCCCTCTTCTCCTT
CCCTTTCGAAAGCATCCTCC

Downstream 100 bases:

>100_bases
TCCAATAGCGACTGCCGGGCCGGTGCCGCCCGGTATGCGATTCCGATCCACAGCCATGACCTACCAACCCATTGAACCTC
CCGACGACGTGAAGGCCGCC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAVAWKSGEPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVTSLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPDATSRFSLDGQPIFHYMGTSTFSNYTVAPEISLARIREDAP
FDKVCYIGCGVTTGIGAVLFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARQFGMTHFINPNE
VENVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSIIIGVAEAGAEISTRPFQLVTGRQWKGSAFGGARGRT
DVPKIVDWYMEGKIDIDSLITHTMPLEDINKGFDLMKRGESIRGVVVY

Sequences:

>Translated_368_residues
MKTKAAVAWKSGEPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVTSLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPDATSRFSLDGQPIFHYMGTSTFSNYTVAPEISLARIREDAP
FDKVCYIGCGVTTGIGAVLFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARQFGMTHFINPNE
VENVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSIIIGVAEAGAEISTRPFQLVTGRQWKGSAFGGARGRT
DVPKIVDWYMEGKIDIDSLITHTMPLEDINKGFDLMKRGESIRGVVVY
>Mature_368_residues
MKTKAAVAWKSGEPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVTSLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPDATSRFSLDGQPIFHYMGTSTFSNYTVAPEISLARIREDAP
FDKVCYIGCGVTTGIGAVLFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARQFGMTHFINPNE
VENVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSIIIGVAEAGAEISTRPFQLVTGRQWKGSAFGGARGRT
DVPKIVDWYMEGKIDIDSLITHTMPLEDINKGFDLMKRGESIRGVVVY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=60.4904632152589, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=45.4545454545455, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=44.7154471544715, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=44.1734417344173, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=44.0540540540541, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=44.0540540540541, Blast_Score=331, Evalue=6e-91,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=43.9024390243902, Blast_Score=327, Evalue=9e-90,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=42.1195652173913, Blast_Score=319, Evalue=2e-87,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=42.382271468144, Blast_Score=313, Evalue=1e-85,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=60.7046070460705, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI87082125, Length=381, Percent_Identity=26.7716535433071, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788073, Length=373, Percent_Identity=27.6139410187668, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790045, Length=359, Percent_Identity=28.4122562674095, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081918, Length=367, Percent_Identity=28.6103542234332, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786825, Length=379, Percent_Identity=24.802110817942, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI226510992, Length=374, Percent_Identity=24.8663101604278, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788075, Length=372, Percent_Identity=27.4193548387097, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=24.4956772334294, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.7956403269755, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.9597855227882, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.6666666666667, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=54.8104956268222, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17562584, Length=366, Percent_Identity=27.5956284153005, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17562876, Length=356, Percent_Identity=25.2808988764045, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=359, Percent_Identity=25.3481894150418, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=374, Percent_Identity=27.0053475935829, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562878, Length=358, Percent_Identity=25.6983240223464, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.177897574124, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319621, Length=373, Percent_Identity=29.7587131367292, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324486, Length=372, Percent_Identity=27.1505376344086, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323729, Length=373, Percent_Identity=27.6139410187668, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323961, Length=365, Percent_Identity=27.3972602739726, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=28.686327077748, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322619, Length=362, Percent_Identity=28.4530386740331, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319955, Length=362, Percent_Identity=28.4530386740331, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319257, Length=355, Percent_Identity=24.2253521126761, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=281, Percent_Identity=27.0462633451957, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=59.7826086956522, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17137530, Length=337, Percent_Identity=27.5964391691395, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39554; Mature: 39554

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAVAWKSGEPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVTSLKKGDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPD
CCCCCEEEEECCCCCCHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
ATSRFSLDGQPIFHYMGTSTFSNYTVAPEISLARIREDAPFDKVCYIGCGVTTGIGAVLF
CCCEEECCCCEEEEEECCCCCCCEEECCCCHHHHHHCCCCCCCEEEEECCHHHHHHHEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARQFGMTHFINPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCCHH
VENVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGRQWKGSAFGGARGRTDVPKIVDWYMEGKIDIDSLITHTMPLEDINKGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHCCHHHHHCCC
SIRGVVVY
CCCEEEEC
>Mature Secondary Structure
MKTKAAVAWKSGEPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVTSLKKGDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPD
CCCCCEEEEECCCCCCHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
ATSRFSLDGQPIFHYMGTSTFSNYTVAPEISLARIREDAPFDKVCYIGCGVTTGIGAVLF
CCCEEECCCCEEEEEECCCCCCCEEECCCCHHHHHHCCCCCCCEEEEECCHHHHHHHEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARQFGMTHFINPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCCHH
VENVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGRQWKGSAFGGARGRTDVPKIVDWYMEGKIDIDSLITHTMPLEDINKGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHCCHHHHHCCC
SIRGVVVY
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]